Use of Bacteriophages to Control<i>Escherichia coli</i>O157:H7 in Domestic Ruminants, Meat Products, and Fruits and Vegetables

Wang, Lili; Qu, Kunli; Li, Xiaoyu; Cao, Zhenhui; Wang, Xitao; Li, Zhen; Song, Yaxiong

doi:10.1089/fpd.2016.2266

Cited by 38 publications

(32 citation statements)

References 75 publications

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“…Previous studies have examined the effectiveness of phage-therapy candidates—or of phage approved for emergency treatment—by relying on the spontaneous generation of highly virulent phage mutants or on isolates of virulent phage from natural sources [ 9 , 40 , 41 , 42 ]. In contrast, the current study suggests that experimental evolution may be an effective method to increase the killing potential of naturally isolated viruses targeted for phage therapy use, where parallel evolution among phage populations [ 43 , 44 ] can illuminate how phage-therapy candidates mechanistically improve killing ability on target bacterial pathogens.…”

Section: Discussionmentioning

confidence: 99%

Parallel Evolution of Host-Attachment Proteins in Phage PP01 Populations Adapting to Escherichia coli O157:H7

et al. 2018

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The emergence of antibiotic resistance has sparked interest in phage therapy, which uses virulent phages as antibacterial agents. Bacteriophage PP01 has been studied for potential bio-control of Escherichia coli O157:H7, its natural host, but in the laboratory, PP01 can be inefficient at killing this bacterium. Thus, the goal of this study was to improve the therapeutic potential of PP01 through short-term experimental evolution. Four replicate populations of PP01 were serially passaged 21 times on non-evolving E. coli O157:H7 with the prediction that the evolved phage populations would adsorb faster and more efficiently kill the host bacteria. Dead-cell adsorption assays and in vitro killing assays confirmed that evolved viruses improved their adsorption ability on E. coli O157:H7, and adapted to kill host bacteria faster than the wildtype ancestor. Sequencing of candidate tail-fiber genes revealed that the phage populations evolved in parallel; the lineages shared two point mutations in gp38 that encodes a host recognition protein, and surprisingly shared a ~600 bp deletion in gp37 that encodes the distal tail fibers. In contrast, no mutations were observed in the gp12 gene encoding PP01’s short tail fibers. We discuss the functional role of the observed mutations, including the possible adaptive role of the evolved deletions. This study demonstrates how experimental evolution can be used to select for viral traits that improve phage attack of an important bacterial pathogen, and that the molecular targets of selection include loci contributing to cell attachment and phage virulence.

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Section: Discussionmentioning

confidence: 99%

Parallel Evolution of Host-Attachment Proteins in Phage PP01 Populations Adapting to Escherichia coli O157:H7

et al. 2018

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“…E. coli O157:H7 is an important foodborne pathogen [1]. Cattle is considered to be the major reservoir and transmitting diseases to humans primarily by eating contaminated food.…”

Section: Discussionmentioning

confidence: 99%

First Isolation and Molecular Characterization of bla CTX-M-121 -producing Escherichia coli O157:H7 Strain Y4-A109 from Cattle in China

Tong

Zhang

et al. 2020

Preprint

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Background: To study the antibiotic resistance, the molecular epidemiology of bovine Escherichia coli ( E.coli ) O157:H7, and exploring the intrinsic relationship among different isolates, we have collected 27 bovine E. coli O157:H7 strains in Xinjiang from 2012 to 2017 and evaluated virulence genes, antibiotic resistance, and pulsed-field gel electrophoresis (PFGE) molecular typing. Results: Of all the 27 bovine E. coli O157:H7 strains analyzed, 21 strains contained at least one virulence gene, 19 strains carried eae gene (70.4%) and 8 of them carrying stx1 + stx2 + eae + hly + tccP . Most strains were sensitive to all the antibiotics tested. However, 4 of which were antibiotic-resistant, and 2 of which possessed multi-drug resistance, including one ESBL-producing strain. This is the first report of the bla CTX-M-121 gene in bovine E. coli O157:H7. Moreover, the bla CTX-M-121 gene can be transmitted horizontally through plasmid between strains. The similarity of PFGE spectra of 27 strains was between 65.8% and 100%. Two types of PFGE were obtained through cluster analysis, including clusters Ⅰ and Ⅱ. Conclusions: E. coli O157:H7 may have undergone clonal propagation in cattle farms as well as cross-regional transmission and horizontal transmission in different regions in Xinjiang China.

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“…Several studies describing such uses have now been published and reviewed. These include the application of phage to control Salmonella species in bean sprouts (Ye et al, 2010 ), broiler carcasses (Fiorentin et al, 2005 ; Atterbury et al, 2007 ), frankfurters (Guenther et al, 2012 ), chicken skin (Hungaro et al, 2013 ), cheese (Modi et al, 2001 ), and melon (Leverentz et al, 2001 ); Listeria monocytogenes on melon (Leverentz et al, 2003 , 2004 ; Hong et al, 2015 ) and cheeses (Guenther and Loessner, 2011 ); Campylobacter on chicken skin (Atterbury et al, 2003 ); and Escherichia coli O157 on retail beef (Wang et al, 2017 ). Commercial production of the first phage for use in foods was in the Netherlands, with the Listex™ P100 product (Carlton et al, 2005 ) which was launched to control Listeria in cheese and meat.…”

Section: Discussionmentioning

confidence: 99%

Prospects for Biocontrol of Vibrio parahaemolyticus Contamination in Blue Mussels (Mytilus edulus)—A Year-Long Study

Onarinde

Dixon

2018

Front. Microbiol.

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Vibrio parahaemolyticus is an environmental organism normally found in subtropical estuarine environments which can cause seafood-related human infections. Clinical disease is associated with diagnostic presence of tdh and/or trh virulence genes and identification of these genes in our preliminary isolates from retail shellfish prompted a year-long surveillance of isolates from a temperate estuary in the north of England. The microbial and environmental analysis of 117 samples of mussels, seawater or sediment showed the presence of V. parahaemolyticus from mussels (100%) at all time-points throughout the year including the colder months although they were only recovered from 94.9% of seawater and 92.3% of sediment samples. Throughout the surveillance, 96 isolates were subjected to specific PCR for virulence genes and none tested positive for either. The common understanding that consuming poorly cooked mussels only represents a risk of infection during summer vacations therefore is challenged. Further investigations with V. parahaemolyticus using RAPD-PCR cluster analysis showed a genetically diverse population. There was no distinct clustering for “environmental” or “clinical” reference strains although a wide variability and heterogeneity agreed with other reports. Continued surveillance of isolates to allay public health risks are justified since geographical distribution and composition of V. parahaemolyticus varies with Future Ocean warming and the potential of environmental strains to acquire virulence genes from pathogenic isolates. The prospects for intervention by phage-mediated biocontrol to reduce or eradicate V. parahaemolyticus in mussels was also investigated. Bacteriophages isolated from enriched samples collected from the river Humber were assessed for their ability to inhibit the growth of V. parahaemolyticus strains in-vitro and in-vivo (with live mussels). V. parahaemolyticus were significantly reduced in-vitro, by an average of 1 log−2 log units and in-vivo, significant reduction of the organisms in mussels occurred in three replicate experimental tank set ups with a “phage cocktail” containing 12 different phages. Our perspective biocontrol study suggests that a cocktail of specific phages targeted against strains of V. parahaemolyticus provides good evidence in an experimental setting of the valuable potential of phage as a decontamination agent in natural or industrial mussel processing (343w).

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Use of Bacteriophages to ControlEscherichia coliO157:H7 in Domestic Ruminants, Meat Products, and Fruits and Vegetables

Cited by 38 publications

References 75 publications

Parallel Evolution of Host-Attachment Proteins in Phage PP01 Populations Adapting to Escherichia coli O157:H7

Parallel Evolution of Host-Attachment Proteins in Phage PP01 Populations Adapting to Escherichia coli O157:H7

First Isolation and Molecular Characterization of bla CTX-M-121 -producing Escherichia coli O157:H7 Strain Y4-A109 from Cattle in China

Prospects for Biocontrol of Vibrio parahaemolyticus Contamination in Blue Mussels (Mytilus edulus)—A Year-Long Study

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