Use of bacterial lipopolysaccharide (LPS) as an immunostimulant for the control of<i>Aeromonas hydrophila</i>infections in rainbow trout<i>Oncorhynchus mykiss</i>(Walbaum)

Nya, Elijah; Austin, Brian

doi:10.1111/j.1365-2672.2009.04464.x

Cited by 72 publications

(55 citation statements)

References 51 publications

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“…For example, LPS, which is a component of the outer cell wall membrane of Gram-negative bacteria, possesses immunogenic properties, and a small dose (i.e., mg ml À1 ; Iliev et al [34]) can induce the production/activation of antibody, lysozyme, alternative complement pathway, B and T lymphocytes, cytokines like interleukin (IL)-2 and -6, pro-inflammatory cytokines like IL-1b, tumour necrosis factor (TNF)-a and several other factors from macrophages, including phagocytic activity in fish [35,36]. OMPs of Gram-negative bacteria are also known to be immunodominant antigens, and may provoke strong humoral and cellular immune responses in fish [37,38].…”

Section: Discussionmentioning

confidence: 99%

Subcellular components of probiotics Kocuria SM1 and Rhodococcus SM2 induce protective immunity in rainbow trout (Oncorhynchus mykiss, Walbaum) against Vibrio anguillarum

Sharifuzzaman¹,

Abbass²,

Tinsley³

et al. 2011

Fish & Shellfish Immunology

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The efficacy of cellular components of probiotics Kocuria SM1 and Rhodococcus SM2 to protect rainbow trout (Oncorhynchus mykiss, Walbaum) against vibriosis was assessed. Groups of fish (average weight = 10–15 g) were immunized intraperitoneally (i.p.) with 0.1 ml of subcellular materials, i.e. 0.2 ± 0.05 mg protein per fish, comprising extracellular proteins (ECPs), cell wall proteins (CWPs) and whole cell proteins (WCPs) of SM1 and SM2, respectively, or with 0.1 ml of phosphate-buffered saline (PBS) to serve as the control. Seven days after administration, fish from each group were challenged i.p. with 0.1 ml of a suspension in PBS of 3 × 105 cells ml−1 per fish of Vibrio anguillarum. Use of CWPs and WCPs demonstrated significantly (P < 0.05) better protection against V. anguillarum insofar as mortalities were reduced to 11–17% [relative percent survival (RPS) = 80–87%], although ECPs fared less well (mortalities = 33–38%; RPS = 56–62%; P > 0.05), compared to 86% mortalities of the controls. The mode of action reflected activation of innate immune factors by CWPs and WCPs, demonstrating significantly (P < 0.05) increased expression of respiratory burst (optical density; OD550nm) from 0.039 to 0.043–0.045, peroxidase (OD550nm) from 0.26 to 0.37–0.55, and bacterial killing activities (i.e. percentage of surviving bacteria reduced from 79% to 56–57% for SM2). Moreover, an elevation of leucocyte number (from 1.93% to 1.98–2.93%; P > 0.05) and immunoglubolin level (from 27 mg ml−1 to 28.5–33 mg ml−1; P > 0.05) were observed with the experimental groups. These results indicate that cell components of the probiotics stimulate an immune response

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Section: Discussionmentioning

confidence: 99%

Subcellular components of probiotics Kocuria SM1 and Rhodococcus SM2 induce protective immunity in rainbow trout (Oncorhynchus mykiss, Walbaum) against Vibrio anguillarum

Sharifuzzaman¹,

Abbass²,

Tinsley³

et al. 2011

Fish & Shellfish Immunology

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“…It was reported that low concentration of LPS could be beneficial to fish for enhancing non-specific immune responses such as phagocytic activity and proliferation of B and T lymphocytes [14]. Rock bream is a commercially important fish in marine aquaculture.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and expression analysis of Cathepsin B and L cysteine proteases from rock bream (Oplegnathus fasciatus)

Whang

Zoysa

Nikapitiya

et al. 2011

Fish & Shellfish Immunology

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“…Antibacterial, lysozyme and phenoloxidase activities were significantly increased when crab Scylla paramamosain was challenged with bacterial LPS (Nya andAustin 2010, Gopalakrishnan et al 2011). The results of this study showed that LPS had a similar effect on Malabar grouper and could be a potential effective immunostimulant for it.…”

Section: Discussionmentioning

confidence: 99%

“…Lipopolysaccharide (LPS), an essential component of the cell wall of Gram-negative bacteria, can effectively improve the non-specific immunity of aquatic and terrestrial animals and is widely used as an immunostimulant to control their infectious diseases (Nya and Austin 2010). Jian et al (2004) reported that, after injection of LPS for 28 days, the immunity of many aquatic animals was significantly improved.…”

Section: Introductionmentioning

confidence: 99%

Analysis of differentially expressed gene fragments in the head kidney of lipopolysaccharide-stimulated Malabar grouper (Epinephelus malabaricus)

Xie

Wang

et al. 2014

Arch. med. vet.

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RESUMENSe estudiaron los efectos inmunológicos y el mecanismo molecular preliminar de lipopolisacáridos (LPS) en el mero malabárico (Epinephelus malabaricus). El mero se inyectó intraperitonealmente dos veces (con 7 días de diferencia) con 4 mg/kg -1 peso corporal de LPS provenientes de Escherichia coli (grupo de prueba, probadores) o solución de tampón fosfato pH 7,2 (grupo control, conductores). Veintiocho días después, la actividad media de antibacterianos, lisozima y polifenol oxidasa del suero mostraron valores de probador de 0,228, 0,032 y 21,8 U/ml x min, respectivamente, mientras que los valores de controlador fueron 0,200, 0,015 y 15,5 U/ml x min. Se elaboró una librería subtractiva de cDNA del riñón cefálico estimulado con LPS de mero malabárico utilizando hibridación substractiva por supresión. Se seleccionaron y secuenciaron 376 clones de fragmentos de genes expresados de probadores específicos, y se obtuvieron 326 EST calificados. Después de una búsqueda con los programas BLASTn y BLASTx, 312 ESTs mostraron gran similitud para 13 fragmentos de genes (14 ESTs no se asemejaron a ninguno de los genes en el GenBank). De los 13 fragmentos, dos (15,4%) estaban relacionados con inmunodefensa (factor 2 regulador de interferón-proteína de unión 2-A y complejo T de proteínas 1-theta); cinco (38,5%) estuvieron relacionados con la transcripción o la traducción; uno (7,7%) estuvo involucrado en el metabolismo (proteína CT054); dos (15,4%) codificarían homologoproteína de diferenciación, leucemia mieloide y la proteína 3 que contiene el dominio parche G; y tres (23,1%) eran genes de transporte de oxígeno que codifican la cadena α de hemoglobina, la cadena β de la hemoglobina y de la cadena pesada de ferritina. Los resultados mostraron que LPS podrían mejorar significativamente la inmunidad innata y regular la expresión de genes relacionados con la inmunidad, la producción de energía celular, el crecimiento, metabolismo o la resistencia al estrés en mero malabárico. Además, LPS pueden ser utilizados como un inmunoestimulante eficaz para esta especie, proporcionando una visión de los mecanismos de los efectos de LPS en animales acuáticos a niveles serológicos y moleculares. Palabras clave: Epinephelus malabaricus, lipopolisacárido (LPS), hibridación subtractiva, inmunoestimulate. SUMMARYThe immunological effect and preliminary molecular mechanism of lipopolysaccharide (LPS) on Malabar grouper (Epinephelus malabaricus) were studied. The grouper was injected intraperitoneally twice (7 days apart) with 4 mg kg -1 body weight of LPS from Escherichia coli (test group; testers) or pH 7.2 phosphate buffer solution (control group; drivers). Twenty-eight days later, the mean antibacterial, lysozyme and polyphenol oxidase activities of the serum showed tester values of 0.228, 0.032 and 21.8 U/ml×min respectively, and driver values of 0.200, 0.015 and 15.5 U/ml×min. A subtracted cDNA library from the head kidney of LPS-stimulated Malabar grouper was constructed using suppression subtractive hybridization. A total of 376 clone...

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Use of bacterial lipopolysaccharide (LPS) as an immunostimulant for the control ofAeromonas hydrophilainfections in rainbow troutOncorhynchus mykiss(Walbaum)

Cited by 72 publications

References 51 publications

Subcellular components of probiotics Kocuria SM1 and Rhodococcus SM2 induce protective immunity in rainbow trout (Oncorhynchus mykiss, Walbaum) against Vibrio anguillarum

Subcellular components of probiotics Kocuria SM1 and Rhodococcus SM2 induce protective immunity in rainbow trout (Oncorhynchus mykiss, Walbaum) against Vibrio anguillarum

Molecular characterization and expression analysis of Cathepsin B and L cysteine proteases from rock bream (Oplegnathus fasciatus)

Analysis of differentially expressed gene fragments in the head kidney of lipopolysaccharide-stimulated Malabar grouper (Epinephelus malabaricus)

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