Use of Bacteria and Yeast Strains for Dairy Wastewater Treatment

Keffala, Chéma; Zouhir, Fouad; Abdallah, Ben Hadj; Kammoun, S.

doi:10.15623/ijret.2017.0603019

IJRET

2017

DOI: 10.15623/ijret.2017.0603019

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Use of Bacteria and Yeast Strains for Dairy Wastewater Treatment

Chéma Keffala¹,

Fouad Zouhir

Ben Hadj Abdallah³

et al.

Abstract: The present investigation was carried out to study the biodegradation of dairy effluent collected from a regional dairy industry, in Tunisia, by using selected aerobic microbial and yeast isolates. 75.8 and 70.7%, respectively, at 1% (v/v)

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Cited by 3 publications

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“…retrieved from spoiled vegetable waste including peas, bulb onion, small onion, tomato, carrot, potato, beetroot, and radish had the potential to oxidize ethanol. Candida albicans, C. krusei, Lactobacillus plantarum, and L. casei were identified from the dairy plant's activated sludge pond(Keffala et al, 2017). Trichosporon loubieri, Geotrichum sp., and T.…”

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confidence: 99%

Screening and Characterization of Potential Ethanol Oxidizing Yeast Isolates From Sugar-Rich Organic Waste Resources Using Schiff’s-Base Reaction

Subashri

Mahalingam²

2023

J microb biotech food sci

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Alcohol dehydrogenase is an oxidoreductase enzyme that carries both alcohol oxidation and aldehyde/ketone reduction activity. Alcohol dehydrogenase (ADH) activity of yeast cells were essential for industrial applications. Sugar-rich organic waste resources including spoiled fruits, fruit peel waste, spoiled vegetables, raw and composed sludge from the dairy industry, and cane molasses spill from the sugar industry, provide a favorable habitat for ethanol-oxidizing microorganisms with ADH activity. The total ethanol oxidizing microbial population of organic waste resources differs considerably from the overall microbial community. Yeast from the natural habitats has a great potential for developing novel enzymes for industrial uses. Under this backdrop, the present study was focused on screening and characterization of potential ethanol oxidizing yeast strains from sugar-rich organic waste resources. Totally seventeen ethanol oxidizing yeast strains belongs to Pichia sp. (9 strains), Candida sp. (2 strains) and Debaryomyces sp. (2 strains), Hanseniaspora sp, Lachancea sp, Torulaspora sp, and Magnusiomyces sp. have been identified. The ability of strains to oxidize ethanol into acetaldehyde was verified by the Schiff’s-base reaction through p-rosaniline assay. The selected five potential ethanol oxidizing strains were identified by 18S rRNA gene sequence as Pichia kluyveri FYES5, Pichia kudriavzevii FYES6, Pichia kudriavzevii VYES2, Candida mengyuniae CSYES1, and Magnusiomyces capitatus CSYES2 with the p-r index of 2.64±0.40, 3.79±0.54, 3.73±0.69, 2.28±0.24, and 2.87±0.58 respectively at 28°C. This is the first report to identify ethanol oxidizing yeast strains from sugar-rich organic waste resources and by this study found that the strain Candida mengyuniae has a better ability to oxidize ethanol.

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mentioning

confidence: 99%

Screening and Characterization of Potential Ethanol Oxidizing Yeast Isolates From Sugar-Rich Organic Waste Resources Using Schiff’s-Base Reaction

Subashri

Mahalingam²

2023

J microb biotech food sci

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Role of Candida in the bioremediation of pollutants: a review

Rana,

Handa,

Aggarwal

et al. 2023

Letters in Applied Microbiology

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The population and modernization of society have increased dramatically from past few decades. In order to meet societal expectations, there has been a massive industrialization and resource exploitation. Anthropogenic practices like disposal of hazardous waste, large carbon footprint release variety of xenobiotic substances into the environment, which endanger the health of the natural ecosystem. Therefore, discovering proper long-term treatment approaches is a global concern. Various physical and chemical approaches are employed to remove contaminants. However, these technologies possess limitations like high cost and low efficacy. Consequently, bioremediation is regarded as one of the most promising remedies to these problems. It creates the option of either totally removing pollutants or transforming them into nonhazardous compounds with the use of natural biological agents. Several microorganisms are being utilized for bioremediation among which yeasts possess benefits such as high biodegradability, ease of cultivation etc. The yeast of Candida genus has the capability to effectively eliminate heavy metal ions, as well as to degrade and emulsify hydrocarbons which makes it a promising candidate for this purpose. The review highlights many potential uses of Candida in various remediation strategies and discusses future directions for research in this field.

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Yarrowia lipolytica CMGB32 Biosurfactants Produced Using n-Hexadecane: Developing Strategies for Environmental Remediation

Csutak,

Nicula,

Lungulescu

et al. 2024

Applied Sciences

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The yeast Yarrowia lipolytica degrades petroleum compounds, including alkanes, via the monoterminal oxidation pathway, the hydrophobic carbon substrate assimilation is mediated by biosurfactants, and extracellular amphiphilic molecules are produced by the yeast cell. This study focuses on the ability of the strain Y. lipolytica CMGB32 to degrade n-hexadecane by producing biosurfactants with high potential for bioremediation. The hydrocarbon-degrading potential of the yeast strain was observed via a 2,6-dichlorophenolindophenol (DCPIP) test in Bushnell–Hass medium with 1% n-hexadecane, and cell hydrophobicity was expressed as microbial adhesion to hydrocarbons (MATH). Biosurfactant production on yeast peptone (YP) with 1% n-hexadecane was estimated after 72 h using the emulsification index (E24%) against toluene. Crude biosurfactant (cell-free broth) stability tests were performed at different temperatures (4 °C, 70 °C) and NaCl concentrations (2–10%). The effects of a biosurfactant on synthetic wastewater remediation comprised the growth curves (OD measurements) of natural heavy metal degrader Rhodotorula mucilaginosa, determination of nutrients (spectrophotometrically), physico-chemical parameters, and removal capacity of lead and cadmium ions (via inductively coupled plasma mass spectrometry—ICP-MS). The antimicrobial and anti-adherence activities of 20 mg/mL and 40 mg/mL of the biosurfactant against pathogenic Candida krusei strains involved growth observations and the crystal violet microtiter method. The DCPIP decolorization occurred after six days, corresponding to the maximum growth phase of the Y. lipolytica culture. After 72 h, the cells presented high hydrophobicity (82.61% MATH) and stable biosurfactant production (E24% 47%). The crude biosurfactant (5%) increased the growth of R. mucilaginosa strains cultivated on synthetic wastewater cultures contaminated with Pb2+ and Cd2+, increased the conductivity and COD (86%) of the samples, and determined Pb2+ (66%) and Cd2+ (42%) ions reduction. The concentrated biosurfactant inhibited C. krusei growth (70%) and biofilm adherence. In conclusion, Y. lipolytica CMGB32 shows important potential for development of biosurfactant-based technologies for the remediation of heavy-metal- and emerging pathogen-contaminated wastewaters.

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Use of Bacteria and Yeast Strains for Dairy Wastewater Treatment

Abstract: The present investigation was carried out to study the biodegradation of dairy effluent collected from a regional dairy industry, in Tunisia, by using selected aerobic microbial and yeast isolates. 75.8 and 70.7%, respectively, at 1% (v/v)

Cited by 3 publications

References 10 publications

Screening and Characterization of Potential Ethanol Oxidizing Yeast Isolates From Sugar-Rich Organic Waste Resources Using Schiff’s-Base Reaction

Screening and Characterization of Potential Ethanol Oxidizing Yeast Isolates From Sugar-Rich Organic Waste Resources Using Schiff’s-Base Reaction

Role of Candida in the bioremediation of pollutants: a review

Yarrowia lipolytica CMGB32 Biosurfactants Produced Using n-Hexadecane: Developing Strategies for Environmental Remediation

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