Use of Bacillus Subtilis PB6 as a potential antibiotic growth promoter replacement in improving performance of broiler birds

Jayaraman, Sathishkumar; Das, Partha Pratim; Saini, Prakash Chandra; Roy, Barun; Chatterjee, P. N.

doi:10.3382/ps/pex079

Cited by 60 publications

(49 citation statements)

References 26 publications

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“…Bacillus subtilis (BAS) spores (Gram‐positive spore‐forming bacteria) are among the most used probiotics in poultry production due to their thermal stability during feed processing and their resistance to the physiological conditions of enteric ecosystem (Boroojeni, Svihus, Reichenbach, & Zentek, ; Li et al, ; Suva, Sureja, & Kheni, ). Positive effects of supplementing broiler diets with BAS strains have been reported in previous studies (Bai et al, ; Jayaraman, Das, Saini, Roy, & Chatterjee, ). These effects may be due to the ability of BAS in modulating the intestinal microbiota in favour of useful organisms, boosting the immune system, modification of the gut structure and morphology, and improving nutrients digestion and absorption (Elshaghabee, Rokana, Gulhane, Sharma, & Panwar, ; Jayaraman et al, ; Khochamit, Siripornadulsil, Sukon, & Siripornadulsil, ; Lee, Kim, Lillehoj, Jang, & Lee, ; Li et al, ).…”

Section: Introductionsupporting

confidence: 60%

“…A review of literature reveals that the improvement in growth performance of birds fed diets supplemented with BAS may be associated with their ability in fine‐tuning gut ecosystem. This may happen through competitive adhesion and synthesis of the antimicrobial compound, immunomodulation, improving intestinal integrity and function, and secretion of digestive enzymes (Jayaraman et al, ; Lee et al, ; Li et al, ; Wang & Gu, ). In our study, histological and microbiology data also indicated that BAS led to improve intestinal mucosa morphology and maintained gut microbiota balance.…”

Section: Discussionmentioning

confidence: 99%

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Effect of dietary supplementation of whey powder and Bacillus subtilis on growth performance, gut and hepatic function, and muscle antioxidant capacity of Japanese quail

Jazi

Farahi

Khajali

et al. 2020

Animal Physiology Nutrition

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This experiment was conducted to evaluate the effects of dietary supplementation of whey powder (WP), Bacillus subtilis (BAS), and their combination (MIX) on growth performance, intestinal morphology, caecal microflora, hepatic gene expression, blood metabolites, and skeletal muscle antioxidant capacity in Japanese quails.A total of 400 one-day-old Japanese quails were randomly distributed to 20-floor pens (4 dietary treatments, 5 replications per treatment, 20 birds per pen). The birds were fed a basal diet (control, CON) or the basal diet supplemented with 40 g/kg WP, 1 g/kg BAS probiotic or 40 g/kg WP plus 1 g/kg BAS probiotic for five weeks. Feed intake was not affected by the treatments at any stage of the trial. However, the WP, BAS, and MIX feed had better weight gain and feed conversion ratio compared to the CON during the entire production period (day 1-35; p < .05). Feeding the WP, BAS, and MIX diets caused no significant difference in morphometric measures in the duodenum, jejunum, and ileum other than the villus height to crypt depth ratio in the ileum (p < .05). The expression of insulin-like growth factor-1 (IGF-1) and growth hormone genes was highly upregulated in the liver of the birds fed the MIX diet (p < .05).Feeding birds with the diets containing WP, BAS, and MIX increased the population of caecal lactic acid bacteria and reduced serum cholesterol concentration compared to the CON diet (p < .05). Likewise, the tested feed additives increased superoxide dismutase and glutathione peroxidase enzyme activities in the thigh muscle (p < .05).No synergistic effect was found between WP and BAS in studied parameters other than IGF-1 gene expression. Improved growth performance of Japanese quails by feeding the WP, BAS, and the MIX feed could be linked to improved absorptive capacity of the small intestine as well as over-expression of anabolic growth factors.In conclusion, WP with or without BAS could be considered as a beneficial dietary supplement to enhance productive performance, gut functionality, and antioxidant capacity of Japanese quail. K E Y W O R D S antioxidant capacity, enteric ecosystem, Japanese quail, probiotic, whey powder | 887 JAZI et Al. How to cite this article: Jazi V, Farahi M, Khajali F, Abousaad S, Ferket P, Assadi Soumeh E. Effect of dietary supplementation of whey powder and Bacillus subtilis on growth performance, gut and hepatic function, and muscle antioxidant capacity of Japanese quail.

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Section: Introductionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Effect of dietary supplementation of whey powder and Bacillus subtilis on growth performance, gut and hepatic function, and muscle antioxidant capacity of Japanese quail

Jazi

Farahi

Khajali

et al. 2020

Animal Physiology Nutrition

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“…The use of probiotics in poultry diets has a direct role in modulating the composition of gut microbiota to promote the growth of beneficial species (11,(41)(42)(43). This study was part of a large project (40,44), that evaluated the efficacy of feeding Shaver White chickens with various levels of B. subtilis DSM 29784 (no probiotic [CON], 1.1Eϩ08 [LSSB], 2.2Eϩ08 [MSSB], or 1.1Eϩ09 [HSSB] CFU/kg of diet) in modulating the growth/ production performance and AR of nutrients across different phases of the growth/ production cycle.…”

Section: Discussionmentioning

confidence: 99%

Bacillus subtilis Strain DSM 29784 Modulates the Cecal Microbiome, Concentration of Short-Chain Fatty Acids, and Apparent Retention of Dietary Components in Shaver White Chickens during Grower, Developer, and Laying Phases

Neijat

Habtewold

Shirley³

et al. 2019

Appl Environ Microbiol

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This study investigated the efficacy of a single strain of Bacillus subtilis (SSB) in modulating the composition of cecal microbiota and its link to the concentration of short-chain fatty acids (SCFA) and apparent retention (AR) of components. A total of 720, 4-week-old Shaver White chicks were allotted to control (CON), 1.1Eϩ08 (low, LSSB), 2.2Eϩ08 (medium, MSSB), or 1.1Eϩ09 (high, HSSB) CFU/kg of diet groups. At grower (10-week), developer (16-week), and laying (28-week) phases, excreta and cecal digesta samples were taken for AR, microbial, and SCFA analyses. Microbial analysis involved high-throughput sequencing of the V3-V4 hypervariable regions of 16S rRNA gene. Bacterial diversity decreased (P Ͻ 0.05) at the developer phase as the SSB dose increased; however, a distinct clustering pattern (P Ͻ 0.05) of bacterial community was noted. Bacteroides and Faecalibacterium were differentially enriched in the developer for SSB-fed compared to CON-fed birds. Although no differences in microbial diversity were detected in grower and layer phases, different species of Clostridium (XVIII, XIVa, IV, and XIVb)-major butyrate producers-were identified in all phases, with stronger effect sizes for SSB-fed compared to CON-fed birds. Isobutyric acid was elevated in dose response (P ϭ 0.034) in layer phase. In addition, the relative abundances of Alistipes, Lactobacillus, and Bifidobacterium were positively correlated (P Ͻ 0.05), with AR of most components for SSB-fed birds in the pullet phase. The results suggested that supplementing chickens' diet with B. subtilis DSM 29784 may selectively enrich beneficial bacterial communities, which in turn are critical in promoting the growth and performance of hens. IMPORTANCE In egg-laying chickens, the trend in the move away from the cage to alternative housing systems and restriction in antimicrobial use requires alternative approaches to maintain health and prevent diseases. There is increased research and commercial interest toward alternative gut health solutions while improving the performance and product safety in poultry production systems. One such approach, in recognition of the importance of the gut microbial community, is the use of microbes as feed supplements (such as probiotics). Unlike meat-type chickens, studies assessing the efficacy of such microbial supplements are limited for egg-laying chickens. Thus, by conducting a comprehensive assessment of the hen microbiota in response to various levels of B. subtilis DSM 29784 during the pullet phase (grower and developer) and the layer phase, the present study demonstrates the importance of direct-fed microbes in modulating gut microbiome, which may relate to improved performance efficiency in the pullet and layer phases.Citation Neijat M, Habtewold J, Shirley RB, Welsher A, Barton J, Thiery P, Kiarie E. 2019. Bacillus subtilis strain DSM 29784 modulates the cecal microbiome, concentration of shortchain fatty acids, and apparent retention of dietary components in Shaver White chickens during grower, developer, and laying ...

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“…The effect of B. subtilis on growth performance improvements by alteration of intestinal microbiota in broilers is well documented (Abudabos, Alyemni, Dafalla, & Khan, ; Aliakbarpour et al., ; Jayaraman et al., ; Jeong & Kim, ; Sen et al., ; Wu et al., ). We also demonstrated that fermented products from B. subtilis could improve growth performance and intestinal morphology in broilers.…”

Section: Discussionmentioning

confidence: 99%

“…Among Bacillus species, Bacillus subtilis is the most commonly used probiotic microorganism in the animal feed industry (Cutting, 2011;Ghadban, 2002). It has been demonstrated that dietary supplementation of B. subtilis can modify intestinal microbiota and enhance growth performance in broilers (Aliakbarpour, Chamani, Rahimi, Sadeghi, & Qujeq, 2012;Jayaraman, Das, Saini, Roy, & Chatterjee, 2017;Jeong & Kim, 2014;Sen et al, 2012;Wu, Zhang, Guo, & Lin, 2011). Furthermore, dietary supplementation of B. subtilis spores in broilers is able to exclude gastrointestinal pathogens, including Escherichia coli and Salmonella spp.…”

mentioning

confidence: 99%

Optimization of surfactin production from Bacillus subtilis in fermentation and its effects on Clostridium perfringens‐induced necrotic enteritis and growth performance in broilers

Cheng

Zhang

Han

et al. 2018

Animal Physiology Nutrition

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Bacillus species are commonly used as probiotics in the poultry feed industry for preventing infectious diseases and improving productivity by altering gastrointestinal microbiota. The growth parameters of Bacillus subtilis for surfactin production in fermentation and the benefits of surfactin on broiler chickens remain unclear. In this study, we examined the growth parameters of B. subtilis in fermentation and evaluated the effects of surfactin from B. subtilis-fermented products on Clostridium perfringens-induced necrotic enteritis and growth performance in broilers. Results showed that the highest viable biomass of B. subtilis was observed at 10% molasses and 2% yeast supplementation during fermentation. The 4- and 6-day fermented B. subtilis products were heat-, acid- and bile-resistant. Furthermore, the 4-day fermented B. subtilis products with the highest surfactin concentration showed the maximal antimicrobial activity against pathogens, including Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and C. perfringens. Dietary B. subtilis-fermented product supplementation in broilers significantly improved intestinal morphology and necrotic lesions under C. perfringens challenge. Bacillus subtilis treatments could enhance broiler productivity, as well as promote bone quality and intestinal morphology. These results together indicate that B. subtilis-fermented products containing surfactin have potential for the development as feed additives and use as possible substitutes for antibiotics to treat C. perfringens in the poultry industry.

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Use of Bacillus Subtilis PB6 as a potential antibiotic growth promoter replacement in improving performance of broiler birds

Cited by 60 publications

References 26 publications

Effect of dietary supplementation of whey powder and Bacillus subtilis on growth performance, gut and hepatic function, and muscle antioxidant capacity of Japanese quail

Effect of dietary supplementation of whey powder and Bacillus subtilis on growth performance, gut and hepatic function, and muscle antioxidant capacity of Japanese quail

Bacillus subtilis Strain DSM 29784 Modulates the Cecal Microbiome, Concentration of Short-Chain Fatty Acids, and Apparent Retention of Dietary Components in Shaver White Chickens during Grower, Developer, and Laying Phases

Optimization of surfactin production from Bacillus subtilis in fermentation and its effects on Clostridium perfringens‐induced necrotic enteritis and growth performance in broilers

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