Use of axenic media to determine antibiotic efficacy against coxiella burnetii

Clay, Katherine; Hartley, M. Gill; Russell, Paul F.; Norville, Isobel H.

doi:10.1016/j.ijantimicag.2017.08.006

Cited by 8 publications

(10 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previously, MICs have been determined against C. burnetii NMII ( 12 ); in this study, the MICs have been extended to include C. burnetii NMI ( Table 1 ). Doxycycline hyclate exhibited the lowest MIC against NMI (0.01 to 0.04 μg/ml) and co-trimoxazole exhibited the highest MIC (8 to 32 μg/ml).…”

Section: Resultsmentioning

confidence: 99%

“…These antibiotics are readily available to the military on deployment and exercise. We and others have explored the efficacy of these alternative antibiotics against C. burnetii in the axenic growth medium ACCM-2 ( 12 – 14 ). However, for an obligate intracellular bacterium, it is important to assess the efficacy of each antibiotic within the intracellular environment.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of the Efficacy of Doxycycline, Ciprofloxacin, Levofloxacin, and Co-trimoxazole Using In Vitro and In Vivo Models of Q Fever

Clay

Hartley

Armstrong

et al. 2021

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Q fever, caused by the intracellular pathogen Coxiella burnetii , is traditionally treated using tetracycline antibiotics, such as doxycycline. Doxycycline is often poorly tolerated and antibiotic resistant strains have been isolated. In this study, we have evaluated a panel of antibiotics (doxycycline, ciprofloxacin, levofloxacin, and, co-trimoxazole) against C. burnetii using in vitro methods (determination of MIC using liquid and solid media; efficacy assessment in a THP cell infection model) and in vivo methods (wax moth larvae and mouse models of infection). In addition, the schedule for antibiotic treatment has been evaluated, with therapy initiated at 24 h pre or post challenge. Both doxycycline and levofloxacin limited overt clinical signs during treatment in the AJ mouse model of aerosol infection, but further studies are required to investigate the possibility of disease relapse or incomplete bacterial clearance after the antibiotics are stopped. Levofloxacin was well tolerated and therefore warrants further investigation as an alternative to the current recommended treatment with doxycycline.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of the Efficacy of Doxycycline, Ciprofloxacin, Levofloxacin, and Co-trimoxazole Using In Vitro and In Vivo Models of Q Fever

Clay

Hartley

Armstrong

et al. 2021

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…Our data may also help develop research priorities within the DMS. Notably military research into Q fever,26 travellers’ diarrhoea,27 bacterial skin infections28 and tuberculosis,18 conditions all seen frequently at Role 4, is currently underway or been recently performed. This research, and our data on the clinical burden, will help inform military specific clinical guidance and policies.…”

Section: Discussionmentioning

confidence: 99%

UK Role 4 military infectious diseases and tropical medicine cases in 2005–2013

Burns

Riley²,

Mason³

et al. 2017

J R Army Med Corps

View full text Add to dashboard Cite

UK service personnel in significant numbers continue to suffer a wide range of infectious diseases, acquired throughout the globe, which often require specialist tertiary infection services to diagnose and manage. Future prospective data collection is recommended to identify trends, which in turn will inform military training needs and future research priorities in the Defence Medical Services (DMS) and allows development of appropriate policies and clinical guidelines for management of DMS personnel with infectious diseases.

show abstract

“…Previous attempts to isolate Wolbachia and examine replication were performed in Schneider’s media at a pH of 6.2–6.5, which may be optimal for insect cells that can buffer the pH level, but not extracellular Wolbachia , which may require pH levels in an axenic media to be similar to intracellular levels of 6.5–7.0. Axenic growth of C. burnetii has fueled important new areas of research to aid in understanding disease transmission, as well as a new set of genetic tools for functional genetic studies of the pathogen [ 28 , 29 , 30 , 31 , 32 , 33 ]. Similar to C. burnetii, a Wolbachia axenic culture and associated toolset have the potential to open similarly broad vistas of research.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic Response of Wolbachia pipientis in a Cell-Free Medium

2020

View full text Add to dashboard Cite

Wolbachia, an obligate intracellular bacterium estimated to infect millions of arthropod species worldwide, is currently being utilized in novel control strategies to limit the transmission of Dengue and Zika viruses. A limitation for Wolbachia-based control approaches is the difficulty of transferring Wolbachia to novel hosts and the lack of tools for the genetic transformation of Wolbachia due to the inability to culture Wolbachia outside the insect host cell in an axenic media. Here, we applied extracellular Wolbachia to phenotypic microarrays to measure the metabolic response of Wolbachia in media formulations with different pH levels and supplementation with Casamino acids. Results suggested a pH of 6.5–6.8 and showed that the supplementation of 1 mg/mL casamino acids increased the survival and longevity of Wolbachia in an axenic medium. In addition, phenotypic microarrays are a useful tool to measure the phenotypic response of Wolbachia under different media conditions, as well as determine specific components that may be required for an axenic medium. This study is an initial step toward the development of a potential Wolbachia axenic culture system.

show abstract

Use of axenic media to determine antibiotic efficacy against coxiella burnetii

Cited by 8 publications

References 16 publications

Evaluation of the Efficacy of Doxycycline, Ciprofloxacin, Levofloxacin, and Co-trimoxazole Using In Vitro and In Vivo Models of Q Fever

Evaluation of the Efficacy of Doxycycline, Ciprofloxacin, Levofloxacin, and Co-trimoxazole Using In Vitro and In Vivo Models of Q Fever

UK Role 4 military infectious diseases and tropical medicine cases in 2005–2013

Phenotypic Response of Wolbachia pipientis in a Cell-Free Medium

Contact Info

Product

Resources

About