Use of autologous adipose‐derived mesenchymal stem cells for creation of laryngeal cartilage

Zhang, Hongji; Voytik‐Harbin, Sherry L.; Brookes, Sarah; Zhang, Lujuan; Wallace, Joseph M.; Parker, Noah P.; Halum, Stacey L.

doi:10.1002/lary.26980

Cited by 15 publications

(15 citation statements)

References 17 publications

(45 reference statements)

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“…We have previously demonstrated that aligned muscle implants and cartilage implants individually integrate with host tissue and differentiate, with no foreign body reaction in a rat model of partial hemilaryngectomy. With the long‐term goal of developing new therapeutic options for restoring or replacing damaged or diseased airway (larynx), we extended those studies with the first application of combined tissue‐engineered cartilage‐muscle implants following partial hemilaryngectomy with and without RLN injury.…”

Section: Discussionmentioning

confidence: 99%

“…Cartilage constructs were fabricated as previously described . Briefly, F344 rat ASCs were suspended in type I oligomeric collagen (4.0 mg/mL) and polymerized.…”

Section: Methodsmentioning

confidence: 99%

“…To begin to address these challenges, our group has previously shown that we can replace thyroid cartilage with a novel oligomeric collagen‐adipose stem cell (ASC) implant that enhances cartilage healing with no inflammatory foreign body reaction. We have also shown that replacement of resected adductor muscle complex with a tissue‐engineered (TE) skeletal muscle implant not only is well tolerated by the animal, similar to the cartilage implant, but that it also improves function (on electromyogram [EMG]) when compared to controls .…”

Section: Introductionmentioning

confidence: 99%

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Motor endplate‐expressing cartilage‐muscle implants for reconstruction of a denervated hemilarynx

Brookes¹,

Voytik‐Harbin

Zhang³

et al. 2018

The Laryngoscope

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Objective Tissue engineering of the larynx requires a complex, multiple tissue layer design. Additionally, spontaneous reinnervation of the larynx after recurrent laryngeal nerve (RLN) injury is often disorganized, resulting in subpar function. This study investigates use of tissue‐engineered cartilage and motor endplate‐expressing (MEE) tissue‐engineered skeletal muscle implants for laryngeal reconstruction and the promotion of organized reinnervation after RLN injury. Methods F344 rat primary muscle progenitor cells (MPCs) were isolated. Three‐dimensional muscle constructs were created by encapsulating MPCs in type I oligomeric collagen under passive tension. Constructs were then cultured in differentiation medium (MPC control constructs) or induced to form motor endplates (MEE constructs) with neurotrophic agents. Three‐dimensional cartilage constructs were created with adipose stem cells differentiated in chondrocyte medium. The muscle and cartilage constructs were implanted into surgically created myochondral defects in the F344 rat larynx with injured or intact (control) RLN. At 1‐, 3‐, and 6‐month timepoints, videolaryngoscopy, electromyography (EMG), histology, and immunohistochemistry were used to assess outcomes. Results At all timepoints, cartilage‐muscle implants were well integrated into host tissue. Functionally, there was increased vocal fold adduction and EMG activity in nerve‐injured rats treated with the MEE constructs when compared to those treated with the MPC control constructs. Motor endplate‐expressing constructs had increased myofiber cross‐sectional area compared to MPC control constructs. Conclusion Although our laboratory previously demonstrated that muscle and cartilage constructs could be used separately for hemilaryngeal reconstruction, this study suggests combining them with the modification of MEEs rather than MPCs, resulting in improved muscle recovery after recurrent laryngeal nerve injury. Level of Evidence NA Laryngoscope, 129:1293–1300, 2019

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Section: Discussionmentioning

confidence: 99%

“…Cartilage constructs were fabricated as previously described . Briefly, F344 rat ASCs were suspended in type I oligomeric collagen (4.0 mg/mL) and polymerized.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Motor endplate‐expressing cartilage‐muscle implants for reconstruction of a denervated hemilarynx

Brookes¹,

Voytik‐Harbin

Zhang³

et al. 2018

The Laryngoscope

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“…Heatmap representing the proportion of publications by year that utilized specific translational research methodologies from construct characterization to human trial to investigate laryngeal tissue engineering 85‐87,91,92,94,96,97,198‐306 …”

Section: Discussionmentioning

confidence: 99%

Tissue engineering applications in otolaryngology—The state of translation

Niermeyer

Rodman

et al. 2020

Laryngoscope Investig Oto

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While tissue engineering holds significant potential to address current limitations in reconstructive surgery of the head and neck, few constructs have made their way into routine clinical use. In this review, we aim to appraise the state of head and neck tissue engineering over the past five years, with a specific focus on otologic, nasal, craniofacial bone, and laryngotracheal applications. A comprehensive scoping search of the PubMed database was performed and over 2000 article hits were returned with 290 articles included in the final review. These publications have addressed the hallmark characteristics of tissue engineering (cellular source, scaffold, and growth signaling) for head and neck anatomical sites. While there have been promising reports of effective tissue engineered interventions in small groups of human patients, the majority of research remains constrained to in vitro and in vivo studies aimed at furthering the understanding of the biological processes involved in tissue engineering. Further, differences in functional and cosmetic properties of the ear, nose, airway, and craniofacial bone affect the emphasis of investigation at each site. While otolaryngologists currently play a role in tissue engineering translational research, continued multidisciplinary efforts will likely be required to push the state of translation towards tissue‐engineered constructs available for routine clinical use. Level of Evidence NA.

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“…Ideally, cell‐based tissue‐engineered laryngeal cartilage would have cells evenly distributed throughout the scaffold, which would fuse with the adjacent tissue (ie, laryngeal muscle) without inducing an inflammatory response. Cell‐based therapies have been shown to repair partial laryngeal defects in vivo 40 . Although autologous chondrocyte implantation is used to repair laryngeal cartilage defect(s) with good results, the main drawbacks are biological and surgical limitations 41 …”

Section: Current Research Activities Relating To Tissue‐engineered Lamentioning

confidence: 99%

Tissue engineering of the larynx: A contemporary review

Chen¹,

Shen²,

Shen³

et al. 2020

Clinical Laboratory Analysis

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Objective Tissue engineering has been a topic of extensive research in recent years and has been applied to the regeneration and restoration of many organs including the larynx. Currently, research investigating tissue engineering of the larynx is either ongoing or in the preclinical trial stage. Methods A literature search was performed on the Advanced search field of PubMed using the keywords: “(laryncheal tissue engineering) AND (cartilage regeneration OR scaffolds OR stem cells OR biomolecules).” After applying the selection criteria, 65 articles were included in the study. Results The present review focuses on the rapidly expanding field of tissue‐engineered larynx, which aims to provide stem cell–based scaffolds combined with biological active factors such as growth factors for larynx reconstruction and regeneration. The trend in recent studies is to use new techniques for scaffold construction, such as 3D printing, are developed. All of these strategies have been instrumental in guiding optimization of the tissue‐engineered larynx, leading to a level of clinical induction beyond the in vivo animal experimental phase. Conclusions This review summarizes the current progress and outlines the necessary basic components of regenerative laryngeal medicine in preclinical fields. Finally, it considers the design of scaffolds, support of growth factors, and cell therapies toward potential clinical application.

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Use of autologous adipose‐derived mesenchymal stem cells for creation of laryngeal cartilage

Abstract: NA. Laryngoscope, 128:E123-E129, 2018.

Cited by 15 publications

References 17 publications

Motor endplate‐expressing cartilage‐muscle implants for reconstruction of a denervated hemilarynx

Motor endplate‐expressing cartilage‐muscle implants for reconstruction of a denervated hemilarynx

Tissue engineering applications in otolaryngology—The state of translation

Tissue engineering of the larynx: A contemporary review

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