Use of antioxidant nanoparticles to reduce oxidative stress in blood storage

Abstract

Oxidative damage by free radicals has a negative effect on blood quality during storage. Antioxidant nanoparticles can prevent oxidative stress. We use SOD-CAT-Alb-PEG-PLGA-nanoparticles to reduce the effects of oxidative stress in blood storage. Electrospray was employed to prepare nanoparticles. Nanoparticles entered the test bags and were kept for 35 days from the time of donation under standard conditions. On target days, experiments were performed on the samples taken. The examination included blood smear, red blood cells count, hemoglobin, hematocrit, K, Fe, glutathione peroxidase, glutathion reductase, glucose-6-phosphate dehydrogenase, prooxidant-antioxidant balance, malondialdehyde, and flow cytometric assay for phosphatidylserine. The repeated measures analysis was performed on samples every week. Morphological changes were less in the test group compared to the control. The quantitative hemolysis profile test showed significant changes in the test and control groups (p < 0.05) in consecutive weeks except for K and Fe. Oxidative stress parameters too showed a significant change during the target days of the examination (p < 0.05

The time-course linkage between hemolysis, redox, and metabolic parameters during red blood cell storage with or without uric acid and ascorbic acid supplementation

“…Optimization of storage conditions and extension of RBC shelf-life is still in the spotlight of blood transfusion research. Antioxidant enhancement to deal with the elevated oxidative stress during storage is a common effort to achieve this goal ( Amen et al, 2017 ; Barzegar et al, 2022 ; Nemkov et al, 2022 ). Uric (UA) and ascorbic acid (AA) are efficient natural antioxidants, the effect of which upon stored RBCs has been studied both distinctly ( Dawson et al, 1980 ; Raval et al, 2013 ; Sanford et al, 2017 ; Tzounakas et al, 2022 ) and in combination ( Bardyn et al, 2020 ; Tzounakas et al, 2022 ).…”

Use Opportunities of Hydrated Fullerene Nanoparticles for Hypothermic Storage of Industrial Oysters

“…Of considerable interest is the possibility of using the antioxidant properties of nanoparticles in the production and preservation technologies of various food products [2][3][4]. Due to their special physicochemical properties, nanoparticles, such as fullerenes, have a unique biological activity: they are able to participate in biological processes as a regulator of reactive oxygen species and as acceptors of free radicals, to protect against oxidative stress [5,6]. It is obvious that long-term hypothermic storage of biological material as a food raw material is accompanied by oxidation processes that contribute to a decrease in the potential of antioxidant protection, biological damage and the destruction of biomolecules.…”

“…The purpose of this study was to evaluate the effect of C60FWS antioxidant properties on the survival of Crassostrea gigas oysters under hypothermia and anoxia according to their physicochemical and biochemical parameters and sensory characteristics (Figure 1). activity: they are able to participate in biological processes as a regulator of reactive oxygen species and as acceptors of free radicals, to protect against oxidative stress [5,6]. It is obvious that long-term hypothermic storage of biological material as a food raw material is accompanied by oxidation processes that contribute to a decrease in the potential of antioxidant protection, biological damage and the destruction of biomolecules.…”

Nanocrystalline Cerium Dioxide Affects Erythrocyte Membrane State Under Blood Hypothermic Storage

“…У отриманих осадах еритроцитів після екстракції у суміші гептану та ізопропанолу (1:1) спектрофотометрично вимірювали концентрацію первинних продуктів ПОЛ, яку розраховували за законом Бугера-Ламберта-Бера у наномолях на 1 мл еритроцитів з використанням коефіцієнта молярної екстинкції ℇо: для дієнових кон'югатів (ДК) ℇ о = 2,2 • 10 5 • М -1 • см -1 , триєнових кон'югатів (ТК) ℇ о = 4,34 • 10 см -1 . Для тетраєнових кон'югатів (ТЕТ) коефіцієнт молярної екстинкції не визначений, тому їх вміст виражали у одиницях молярної екстинкції на 1 мл еритроцитів [2,3,6].…”

“…The use of nanoparticles (NPs) for blood storage is being studied in many laboratories worldwide [2,24]. The superoxide ions are known to be neutralized in erythrocytes during blood storage, thereby resulting in cell destruction.…”