The knowledge of the final‐year dental students on antibiotic use in endodontics in Turkey was assessed. The study was conducted at 20 Dentistry Faculties. A questionnaire was sent to the universities including the concerning several scenarios about endodontic cases. 1113 final‐year dental students participated in the study. Respondents’ significant choice was not to administrate antibiotics in endodontic treatments of symptomatic irreversible pulpitis, pulp necrosis, acute apical periodontitis, chronic apical abscess with or without sinus tract, retreatment, inadequate local anaesthesia and patient requests (P > 0.05). In acute apical abscess (AAA) with diffuse cases, choice of antibiotic administration was statistically higher (P < 0.05), while in AAA‐localised cases, the ratio of antibiotic administration versus non‐administration was similar. Amoxicillin was found to be the first‐choice antibiotic in patients without medical allergies, whereas in allergic patients the first choice was clindamycin. Final‐year dental students have fundamental knowledge on the antibiotic administration in endodontics.