Use of anti-idiotypic antibodies to identify a receptor for the T-cell I-J determinant.

Abstract

In order to identify the molecule(s) interacting with the I-J determinant on suppressor T cells, we have generated two anti-idiotypic sera: one to monoclonal anti-I-Jd antibody and one to monoclonal anti-I-PJ antibody. These antisera specifically block suppressor T-cell function in a genetically restricted manner and have no effect on helper T-cell activation. Both recognize a marker on primary monocytes and B cells but not on T cells. A myeloma cell line bearing this marker has been identified. Therefore, these antisera may recognize a molecule on cells interacting with suppressor T cells that is involved in mediating suppressor T-cell activity. The relationship between the T-cell I-J determinant and the molecule identified by the anti-idiotype may be similar to the relationship between the receptor on helper T cells and Ia molecules.

Regulation of immune responses by I-J gene products. VI. Recognition of I-E molecules by I-J-bearing suppressor factors.

“…Moreover, we would predict that anti-idiotype antibodies directed against anti-I-J (anti-antetope) mAb should react with class II epitopes. Anti-idiotype antisera directed against either anti-I-J k (WF8.C12.8) or anti-I-J d (WF18.2B15) mAb bind B cells and macrophages (42). This same anti-idiotype blocks GT-TsF1mediated suppression, in a manner similar to that of the present report.…”

The double edged sword of the immune response: mutational analysis of a murine anti-pneumococcal, anti-DNA antibody.

“…6-8-wk-old BALB/c female mice were primed intraperitoneally with 100 ng of purified anti-I-J d in complete Freund's adjuvant obtained from Difco (Detroit, MI). The anti-I-J d antibody is an IgG2b antibody produced by the WF18.2b15 cell line previously described (19). The antibody was purified from ascites fluid using a protein A-Sepharose column.…”

“…The antibody was purified from ascites fluid using a protein A-Sepharose column. The anti-I-J d antibody recognizes a determinant present on a subset of T cells, and immunization with this antibody enables sampling of previously suppressed immune responses (19).…”

Anti-idiotypic antibodies to rubella virus

“…In turn, an anti-idiotypic antibody (Ab2) could be anticipated to have a structure analogous to that of the epitope of the antigen. This potential use has prompted several laboratories to produce Ab2 against antiviral antibodies [8,20,23,27,33,38,39,44], and has been used successfully for Ab2 binding to the reovirus receptor and to nonviral receptors [9,29,35,50].…”