Use of an Optical-Fiber-Based Biosensor to Study the Interaction of Blood Proteins with Solid Surfaces

Poirier, Michelle A.; Lopes, Teresa Teixeira; Singh, Bal Ram

doi:10.1366/0003702944030026

Cited by 14 publications

(3 citation statements)

References 18 publications

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“…using cationic latex [135] Study of peptide-prptein interaction by surface plasmon resonance (SPR) [ 146] Det. of mismatch formation effects on DNA hybridization kinetics [186] Fiber-optic biosensor for fluorimetric detection of DNA hybridization [360] Fiber bios, to study the interact, of blood proteins with solid surfaces [361] Food safety applications of nucleic acid-based assays [95] [195] Fluores. analogs: opt.…”

Section: Dna Proteinsmentioning

confidence: 99%

Opto-Chemical and Opto-Immuno Sensors

Gauglitz¹

1996

Sensors Update

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Section: Dna Proteinsmentioning

confidence: 99%

Opto-Chemical and Opto-Immuno Sensors

Gauglitz¹

1996

Sensors Update

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“…Recently, the feasibility of using an ATR-IR microscope as a detection unit has been also demonstrated . Several coating materials like polymers, – biological materials, – and sol–gel films , have been used to increase the detection limits of the ATR-IR technique for quantification. The coating has the advantage to increase the population density of molecules nearby the internal reflection element (IRE) used as waveguide for the infrared radiation.…”

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confidence: 99%

Enantioselective Interactions at the Solid–Liquid Interface of an HPLC Column under Working Conditions

2008

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A technique is presented which allows studying the enantioselective interactions occurring at the solid-liquid interface of a chiral stationary phase (CSP) and a racemate relevant to high performance liquid chromatography (HPLC). A conventional chiral column (Chiralpak AS) was mounted on an attenuated total reflection-infrared (ATR-IR) cell mimicking an HPLC setup equipped with an ATR-IR detector. Racemic pantolactone (PL) was used as the selectand. This setup in combination with modulation excitation spectroscopy (MES) allows for the identification of inter- and intramolecular hydrogen bonds being crucial for enantioseparation under HPLC operation conditions. The method is based on a two step strategy. In a first step, the enantiomers are separated by the chiral column similar to a standard HPLC experiment and upon adsorption on the identical CSP deposited on the internal reflection element (IRE), they are detected by ATR-IR spectroscopy. This experiment provides a retention time for each enantiomer. From the difference in retention, a suitable frequency is calculated which is used in a second experiment where the racemate concentration is varied alternately (modulation) in a way that the pulses of ( R)-PL and ( S)-PL exhibit a phase lag of 90 degrees after elution through the column. This procedure allows one to gain separate information of the enantioselective selectand-CSP interaction after performing a demodulation similar to a phase sensitive detection (PSD). A further benefit of this method is the strong enhancement of the signal-to-noise ratio. The effectiveness of the method is demonstrated by investigating the observed faster decrease in retention time of the later-eluted ( R)-PL, as compared to ( S)-PL, when separating at higher temperatures (from 12 to 36 degrees C). The origin is attributed to a weakening of a specific hydrogen bond between the C=O of ( R)-PL and the N-H of the CSP.

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“…Descriptions of the above-mentioned devices can be found in the literature; several fiber probes have been developed for the detection of compounds in blood, such as ferritin [87], albumin [88,89], and lipoproteins [90].…”

Section: Fluorescence Sensorsmentioning

confidence: 99%

Immunosensors for Diagnostics

2001

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Immunosensors can play an important role in the improvement of public health by providing applications for which rapid detection, high sensitivity, and specificity are important, in areas such as clinical chemistry, food quality, and environmental monitoring. The standard immunoassay techniques are the basis for the development of improved automated devices, exploring the accumulated know how of the implementation of indirect detection methods during the last 40 years. Today's systems achieve high accuracy, specificity, and sample throughput. Disposable immunotests (qualitative and quantitative), based on colored detection of an immunoreaction, are continuously expanding its applications for diagnosis of diseases, drug detection, and food quality control.Associated to the increasing demand of immunoapplications and with the new advances produced in the last decades in fields like biochemistry, biotechnology, electronics, and microfabrication, new transduction devices have been developed and applied to monitor immunoreactions. Optimization of the biochemical characteristics of the binding molecules, improved surface stability of the transducer interfaces, and reliable microsystems and integrated devices are the challenge for the next years of development. An overview of the immunoassay techniques used today in standard diagnosis will be presented, including a brief description of the different immunosensor transducers and some examples of the present developments and commercial devices.

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Use of an Optical-Fiber-Based Biosensor to Study the Interaction of Blood Proteins with Solid Surfaces

Cited by 14 publications

References 18 publications

Opto-Chemical and Opto-Immuno Sensors

Opto-Chemical and Opto-Immuno Sensors

Enantioselective Interactions at the Solid–Liquid Interface of an HPLC Column under Working Conditions

Immunosensors for Diagnostics

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