Use of an Interferon Gamma Release Assay (IGRA) to test T-cell responsiveness to soluble Leishmania infantum antigen in whole blood of dogs from endemic areas

Zribi, Lilia; Goulli, Amel Founa El; Benabid, Meriem; Gharbi, Mohamed; Ben-Sghaier, Ines; I, Boufaden; Karim, Abdul; Bouratbine, A.

doi:10.1016/j.vetpar.2017.08.029

Cited by 5 publications

(4 citation statements)

References 27 publications

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“…Assays measuring IFN-γ allow quantifying the level of stimulation of a specific Th1-polarity immune memory response to Leishmania antigen (Fernández-Bellón et al, 2005 ; Rodríguez-Cortés et al, 2007a ; Moreno et al, 2014 ; Zribi et al, 2017 ). The IFN-γ cytophatic effect inhibition bioassay detects the production of IFN-γ by circulating lymphocytes in cultured supernatants incubated in the presence/absence of SLA followed by incubation with canine kidney cells.…”

Section: Cellular Biomarkers To Leishmania Infantum mentioning

confidence: 99%

“…The IFN-γ release assay (IGRA) allows rapid screening of IFN-γ-secretion in whole blood challenged with SLA. It seems to be a useful tool to assess exposure to Leishmania as positive IGRA responses were seen in infected dogs without or with mild clinical signs and in dogs without or with low parasite load, whereas negative IGRAs were identified in dogs with the highest parasitism (Zribi et al, 2017 ). The lack of IFN-γ production in dogs with severe clinical disease and high number of parasites on blood has also been reported (Solano-Gallego et al, 2016 ; Martínez-Orellana et al, 2017 ).…”

Section: Cellular Biomarkers To Leishmania Infantum mentioning

confidence: 99%

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Biomarkers Associated With Leishmania infantum Exposure, Infection, and Disease in Dogs

Maia

Campino

2018

Front. Cell. Infect. Microbiol.

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Canine leishmaniosis (CanL) is a vector-borne disease caused by the protozoan Leishmania (Leishmania) infantum species [syn. L. (L.) infantum chagasi species in the Americas] which is transmitted by the bite of a female phlebotomine sand fly. This parasitosis is endemic and affect millions of dogs in Asia, the Americas and the Mediterranean basin. Domestic dogs are the main hosts and the main reservoir hosts for human zoonotic leishmaniosis. The outcome of infection is a consequence of intricate interactions between the protozoan and the immunological and genetic background of the host. Clinical manifestations can range from subclinical infection to very severe disease. Early detection of infected dogs, their close surveillance and treatment are essential to control the dissemination of the parasite among other dogs, being also a pivotal element for the control of human zoonotic leishmaniosis. Hence, the identification of biomarkers for the confirmation of Leishmania infection, disease and determination of an appropriate treatment would represent an important tool to assist clinicians in diagnosis, monitoring and in giving a realistic prognosis to subclinical infected and sick dogs. Here, we review the recent advances in the identification of Leishmania infantum biomarkers, focusing on those related to parasite exposure, susceptibility to infection and disease development. Markers related to the pathogenesis of the disease and to monitoring the evolution of leishmaniosis and treatment outcome are also summarized. Data emphasizes the complexity of parasite-host interactions and that a single biomarker cannot be used alone for CanL diagnosis or prognosis. Nevertheless, results are encouraging and future research to explore the potential clinical application of biomarkers is warranted.

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Section: Cellular Biomarkers To Leishmania Infantum mentioning

confidence: 99%

Section: Cellular Biomarkers To Leishmania Infantum mentioning

confidence: 99%

Biomarkers Associated With Leishmania infantum Exposure, Infection, and Disease in Dogs

Maia

Campino

2018

Front. Cell. Infect. Microbiol.

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“…This process allows the host to combat the parasite and manage the advancement of the disease [107]. In general, dogs that experience clinical disease and poor clinical outcomes typically exhibit a reduction or absence of the cell-mediated immune response [114][115][116]. In ʺresistantʺ dogs, the Th1 immune response is dominant and is marked by Interferon-γ.…”

Section: Immunological Response In Visceral Leishmaniasis In the Dogmentioning

confidence: 99%

The Immune Response in Canine and Human Leishmaniasis and How This Influences the Diagnosis- a Review and Assessment of Recent Research

Martinescu¹

2023

Preprint

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Laboratory diagnosis of leishmaniasis remains a major challenge, demonstrating variable efficacy in detecting infected mammalian hosts. Thus, there is a great need for the accuracy of the identification of appropriate antigens to also improve diagnostic tests. This review brings to the fore the sensitivity of the balance in canine and human leishmaniasis, which greatly influences the progression of the disease and especially the diagnostic methods. The problem of diagnosis arises in asymptomatic leishmaniasis, since in these conditions the methods considered as reference in the diagnosis of leishmaniasis no longer show certainty, being influenced for the most part by the immune response of the host, which is different according to the presence of other associated diseases or even according to the breed, when we talk about dogs. Consequently, the diagnosis and surveillance of leishmaniasis cases remains an open topic, with the need for new methods adapted to the immunological status of the host.

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“…This process allows the host to combat the parasite and manage the advancement of the disease ( Toepp and Petersen, 2020 ). In general, dogs that experience clinical disease and poor clinical outcomes typically exhibit a reduction or absence of the cell-mediated immune response ( Solano-Gallego et al., 2016 ; Martínez-Orellana et al., 2017 ; Zribi et al., 2017 ). In “resistant” dogs, the Th1 immune response is dominant and is marked by IFN-γ.…”

Section: Immunological Response Of Visceral Leishmaniasis In Dogsmentioning

confidence: 99%

The immune response in canine and human leishmaniasis and how this influences the diagnosis- a review and assessment of recent research

Ivănescu,

Andronic,

Grigore-Hristodorescu

et al. 2023

Front. Cell. Infect. Microbiol.

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Leishmaniasis is a widespread but still underdiagnosed parasitic disease that affects both humans and animals. There are at least 20 pathogenic species of Leishmania, most of them being zoonotic. The diagnosis of leishmaniasis remains a major challenge, with an important role being played by the species of parasites involved, the genetic background, the immunocompetence of the host. This paper brings to the fore the sensitivity of the balance in canine and human leishmaniasis and addresses the importance of the host’s immune response in establishing a correct diagnosis, especially in certain cases of asymptomatic leishmaniasis, or in the situation the host is immunosuppressed or acquired leishmaniasis through vertical transmission. The methods considered as a reference in the diagnosis of leishmaniasis no longer present certainty, the diagnosis being influenced mostly by the immune response of the host, which differs according to the presence of other associated diseases or even according to the breed in dogs. Consequently, the diagnosis and surveillance of leishmaniasis cases remains an open topic, requiring new diagnostic methods adapted to the immunological state of the host.

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Use of an Interferon Gamma Release Assay (IGRA) to test T-cell responsiveness to soluble Leishmania infantum antigen in whole blood of dogs from endemic areas

Cited by 5 publications

References 27 publications

Biomarkers Associated With Leishmania infantum Exposure, Infection, and Disease in Dogs

Biomarkers Associated With Leishmania infantum Exposure, Infection, and Disease in Dogs

The Immune Response in Canine and Human Leishmaniasis and How This Influences the Diagnosis- a Review and Assessment of Recent Research

The immune response in canine and human leishmaniasis and how this influences the diagnosis- a review and assessment of recent research

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