Use of an integrated biomarker-based strategy to evaluate physiological stress responses induced by environmental concentrations of caffeine in the Mediterranean mussel Mytilus galloprovincialis

Capolupo, Marco; Valbonesi, Paola; Kiwan, Alisar; Buratti, Sara; Franzellitti, Silvia; Fabbri, Elena

doi:10.1016/j.scitotenv.2016.04.125

Cited by 57 publications

(26 citation statements)

References 72 publications

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“…The MDA content was quantified in digestive glands according to Franzellitti et al [ 32 ] by interpolating the data of absorbance at 570 nm on a standard curve obtained using the MDA precursor 1,1,3,3-Tetramethoxypropan (TMOP, Sigma-Aldrich ® , Milan, Italy). GST and CAT activities were measured in both digestive glands and gills according to Capolupo et al [ 33 ]. GST activity was evaluated at 340 nm by following the reaction kinetics between the enzyme and the substrate 1-chloro-2,4-dinitrobenzene (CDNB, Sigma-Aldrich ® , Milan, Italy) for 10 min (at 1 min intervals).…”

Section: Methodsmentioning

confidence: 99%

A Comparative Assessment of the Chronic Effects of Micro- and Nano-Plastics on the Physiology of the Mediterranean Mussel Mytilus galloprovincialis

2021

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The ocean contamination caused by micro- and nano-sized plastics is a matter of increasing concern regarding their potential effects on marine organisms. This study compared the effects of a 21-day exposure to 1.5, 15, and 150 ng/L of polystyrene microplastics (PS-MP, 3-µm) and nanoplastics (PS-NP, 50-nm) on a suite of biomarkers measured in the Mediterranean mussel Mytilus galloprovincialis. Endpoints encompassed immunological/lysosomal responses, oxidative stress/detoxification parameters, and neurotoxicological markers. Compared to PS-MP, PS-NP induced higher effects on lysosomal parameters of general stress. Exposures to both particle sizes increased lipid peroxidation and catalase activity in gills; PS-NP elicited greater effects on the phase-II metabolism enzyme glutathione S-transferase and on lysozyme activity, while only PS-MP inhibited the hemocyte phagocytosis, suggesting a major role of PS particle size in modulating immunological/detoxification pathways. A decreased acetylcholinesterase activity was induced by PS-NP, indicating their potential to impair neurological functions in mussels. Biomarker data integration in the Mussel Expert System identified an overall greater health status alteration in mussels exposed to PS-NP compared to PS-MP. This study shows that increasing concentrations of nanoplastics may induce higher effects than microplastics on the mussel’s lysosomal, metabolic, and neurological functions, eventually resulting in a greater impact on their overall fitness.

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Section: Methodsmentioning

confidence: 99%

A Comparative Assessment of the Chronic Effects of Micro- and Nano-Plastics on the Physiology of the Mediterranean Mussel Mytilus galloprovincialis

2021

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“…Although AChE activity was not assessed, very similar responses were obtained in the freshwater clam Corbicula fluminea exposed for 21 days to the same caffeine concentrations tested in R. philippinarum [54]. Impairment in lysosomal membrane stability of hemocytes after exposure to caffeine at doses as low as 0.05-0.1 µg/L suggest that immune cells can be a target for this substance in invertebrates [52][53][54][55], showing immune modulatory effects like those observed in rodents and humans [56].…”

Section: Discussionmentioning

confidence: 68%

“…In M. galloprovincialis, exposure to 0.005, 0.05 and 0.5 µg/L of caffeine for 7 days triggered a significant decrease of lysosomal membrane stability in both hemocytes and digestive glands (at 0.05 and 0.5 µg/L) and a significant increase of lysosomal content of neutral lipids (at 0.5 µg/L). A significant increase in the activity of the biotrasformation enzyme glutathione-S-transferase was also observed in digestive glands (at 0.005 and 0.5 µg/L) [52].…”

Section: Discussionmentioning

confidence: 72%

“…Additionally, Bibby et al [61] found increased levels of phagocytosis in M. edulis exposed to reduced pH values. In a recent study [52], a 7-day exposure of M. galloprovincialis to caffeine caused a significant decrease in lysosomal membrane stability evaluated by neutral red retention time (NRRT) at the concentration of 0.5 µg/L. Similarly, a significant NRRT decrease was found in R. philippinarum exposed for 14 days to 0.1 µg/L [54].…”

Section: Discussionmentioning

confidence: 93%

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Exposure to Decreased pH and Caffeine Affects Hemocyte Parameters in the Mussel Mytilus galloprovincialis

Munari

Matozzo

Benetello

et al. 2020

JMSE

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Combined effects of reduced pH, as predicted under climate change scenarios, and the most popular and widely used stimulant caffeine were assessed in hemocyte parameters of the mussel Mytilus galloprovincialis, being hemocytes involved in immune defense. Bivalves were exposed for one week to natural pH (8.1) and two reduced pH values (pH −0.4 units and pH −0.7 units). Exposure continued for additional two weeks, both in the absence and in the presence of environmentally relevant concentrations of caffeine (0.05 and 0.5 µg/L). Hemocyte parameters (total hemocyte count, hemocyte volume and diameter, neutral red uptake and hemocyte proliferation) were measured after 7 days of exposure to pH only, and after 14 (T1) and 21 (T2) days of exposure to the various pH*caffeine combinations. At all sampling times, pH significantly affected all the biological variables considered, whereas caffeine exhibited a significant influence at T2 only. Among the various hemocyte parameters, caffeine caused a significant increase in total hemocyte count at T2, and in hemocyte volume and diameter at both T1 and T2, when a significant interaction between pH and caffeine was also found. Overall, results demonstrated that hemocyte functionality was strongly influenced by the experimental conditions tested. Further studies are needed to assess combined effects of climate changes and emerging contaminants on bivalve immune system when challenged with environmental pathogens.

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“…(Farombi et al, 2007). Durante o verão de 2009, houve inibição de ChE, a qual está geralmente associada à presença de pesticidas (Capolupo et al, 2016;Valbonesi et al, 2003). (Berbel et al, 2015;Cesar et al, 2007;Hortellani et al, 2008;Yogui et al, 2003).…”

Section: Discussionunclassified

Efeitos biológicos da contaminação sobre bivalves filtradores de regiões do litoral de São Paulo, com ênfase na Baixada Santista

Camargo¹

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Use of an integrated biomarker-based strategy to evaluate physiological stress responses induced by environmental concentrations of caffeine in the Mediterranean mussel Mytilus galloprovincialis

Cited by 57 publications

References 72 publications

A Comparative Assessment of the Chronic Effects of Micro- and Nano-Plastics on the Physiology of the Mediterranean Mussel Mytilus galloprovincialis

A Comparative Assessment of the Chronic Effects of Micro- and Nano-Plastics on the Physiology of the Mediterranean Mussel Mytilus galloprovincialis

Exposure to Decreased pH and Caffeine Affects Hemocyte Parameters in the Mussel Mytilus galloprovincialis

Efeitos biológicos da contaminação sobre bivalves filtradores de regiões do litoral de São Paulo, com ênfase na Baixada Santista

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