Use of an in vitro gas production method to assess differences in microbial fermentation characteristics of feed ingredients and betaine additives for pig nutrition

Rink, F.; Bauer, Eva; Eklund, M.; Hartung, Karin; Mosenthin, R.

doi:10.1016/j.anifeedsci.2011.07.008

Cited by 14 publications

(25 citation statements)

References 11 publications

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“…Accordingly, Rink et al. (, ), using the same in vitro procedure as reported herein, with soybean meal and casein as protein sources to obtain diets with varying CP content (150 g/kg DM CP for the soybean meal‐based diet and 280 g/kg DM CP for the casein‐based diet), found increased ammonia production at the higher protein level. Interestingly, in the present study, no increase in ammonia production due to higher CP level could be observed.…”

Section: Discussionmentioning

confidence: 78%

“…For comparison, inulin was added at the same concentration as the micronized fibres to the basal substrates, because inulin is known for its prebiotic properties such as stimulation of microbial fermentation as measured, for example, by increased SCFA production (e.g. Rink et al., ,b). The air‐dried assay substrates were ground to pass a 1‐mm sieve.…”

Section: Methodsmentioning

confidence: 99%

“…Equal amounts (wet weight basis) of faeces of each animal were pooled, thoroughly mixed thereafter for 90 s using a hand blender to achieve a homogenous mix, and diluted at a ratio of 1 : 60 with an anaerobic sterile buffer, as described in detail by Rink et al. (). The diluted mixture was then filtered through a piece of clean cheesecloth, and the resultant filtrates were used as inocula.…”

Section: Methodsmentioning

confidence: 99%

“…Gas production was measured according to the in vitro method described by Rink et al. (), using faeces from pigs as microbial inoculum. The fermentation glass syringes, containing either the inoculum and normal buffered solution, or inoculum and buffer solution containing 0.4 m NaCl to simulate osmotic stress conditions (Rink et al., ), were incubated, together with the assay substrates, at 39 °C for 24 h. For blanks, only inoculum without any added assay substrate was incubated.…”

Section: Methodsmentioning

confidence: 99%

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Micronized fibres affect in vitro fermentation under normal buffered and osmotic stress conditions using porcine inocula

Aumiller

Mosenthin

Rink

et al. 2015

Animal Physiology Nutrition

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In this in vitro study, the modified Hohenheim gas test was used to determine fermentation activity and bacterial composition of pig's faecal microbial inoculum, when fermenting a standard pig diet with varying levels of crude protein (CP; 20, 24 and 28% CP), and supplemented with one of three fibre sources manufactured by micronization treatment. These were wheat envelopes (MWE), pea fibre (MPF) and lupine fibre (MLF). For comparison, inulin was used. As intestinal bacteria have to cope with varying osmotic conditions in their ecosystem, fermentation was performed under normal buffered and osmotic stress conditions. After 24 h of fermentation, total gas production and ammonia production were measured. In addition, the effect of MWE and inulin on short-chain fatty acid (SCFA) production and numbers of total eubacteria, Lactobacillus spp., Bifidobacterium spp., Enterobacteriaceae, Enterococcus spp., Clostridium cluster XIVa and Clostridium cluster IV, were determined using quantitative real-time PCR. Under normal buffered conditions, supplementation of MWE resulted in increased (p < 0.05) SCFA, acetic, propionic and valerianic acid production at CP levels of 20 and 28%. There was an increase (p < 0.05) in ammonia production for the micronized supplements, and for MWE an increased (p < 0.05) branched-chain proportion was observed, possibly due to higher availability of protein for fermentation which was released during the micronization process. Osmotic stress conditions reduced (p < 0.05) total gas as well as total SCFA, acetic and propionic acid production for all treatments, while cell counts were increased (p < 0.05) for Bifidobacterium spp., Enterococcus spp. and Lactobacillus spp. Under normal buffered conditions in combination with 24 and 28% CP levels, lactobacilli were increased for MWE, compared to inulin (p < 0.05). In conclusion, micronized supplements such as MWE may beneficially modulate pigs' intestinal microbiota by increasing SCFA production in addition to a selective proliferation of lactobacilli.

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Section: Discussionmentioning

confidence: 78%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Micronized fibres affect in vitro fermentation under normal buffered and osmotic stress conditions using porcine inocula

Aumiller

Mosenthin

Rink

et al. 2015

Animal Physiology Nutrition

Self Cite

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show abstract

“…42,45,46 Like the rumen, the large intestine of simple-stomached animals is essentially a fermentation chamber where material is degraded by bacteria. 47 A cumulative gas production technique was used to test a range of different products to assess the end-products of in vitro fermentation in pigs.…”

Section: ■ In Situ and In Vitro Methodsmentioning

confidence: 99%

State of the Art in Feedstuff Analysis: A Technique-Oriented Perspective

Cheli

Battaglia

Pinotti

et al. 2012

J. Agric. Food Chem.

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The need for global feed supply traceability, the high-throughput testing demands of feed industry, and regulatory enforcement drive the need for feed analysis and make extremely complex the issue of the control and evaluation of feed quality, safety, and functional properties, all of which contribute to the very high number of analyses that must be performed. Feed analysis, with respect to animal nutritional requirements, health, reproduction, and production, should be multianalytically approached. In addition to standard methods of chemical analysis, new methods for evaluation of feed composition and functional properties, authenticity, and safety have been developed. Requirements for new analytical methods emphasize performance, sensitivity, reliability, speed, simplified use, low cost for high volume, and routine assays. This review provides an overview of the most used and promising methods for feed analysis. The review is intentionally focused on the following techniques: classical chemical analysis; in situ and in vitro methods; analytical techniques coupled with chemometric tools (NIR and sensors); and cell-based bioassays. This review describes both the potential and limitations of each technique and discusses the challenges that need to be overcome to obtain validated and standardized methods of analysis for a complete and global feed evaluation and characterization.

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The effect of betaine on in vitro fermentation of carbohydrate and protein combinations under osmotic stress in pigs

Rink¹,

Bauer²,

Eklund³

et al. 2012

J Sci Food Agric

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These results suggest that only supplementation of a native betaine source but not of pure betaine products affects microbial fermentation under osmotic stress. Furthermore, both the carbohydrate and protein source play an important role in modifying microbial activity under osmotic stress conditions. It can also be concluded that the modified Hohenheim gas test is suitable to scrutinise fermentation activity of different assay substrates under osmotic stress.

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Use of an in vitro gas production method to assess differences in microbial fermentation characteristics of feed ingredients and betaine additives for pig nutrition

Cited by 14 publications

References 11 publications

Micronized fibres affect in vitro fermentation under normal buffered and osmotic stress conditions using porcine inocula

Micronized fibres affect in vitro fermentation under normal buffered and osmotic stress conditions using porcine inocula

State of the Art in Feedstuff Analysis: A Technique-Oriented Perspective

The effect of betaine on in vitro fermentation of carbohydrate and protein combinations under osmotic stress in pigs

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