Use of an Exotic Carbon Source To Selectively Increase Metabolic Activity and Growth of
            <i>Pseudomonas putida</i>
            in Soil

Colbert, Stephen F.; Isakeit, Thomas; Ferri, Mario; Weinhold, A. R.; Hendson, M.; Schroth, M. N.

doi:10.1128/aem.59.7.2056-2063.1993

Cited by 34 publications

(16 citation statements)

References 19 publications

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“…Our results suggest that the ability of fluorescent pseudomonads to utilize specific organic substrates may be one important bacterial trait involved in the selection of soilborne fluorescent pseudomonads achieved by the plant. When introduced into the rhizosphere, a bacterial inoculant having the ability to utilize a compound present in the root exudate should have a competitive advantage over soil bacteria without this ability (6,22). Other bacterial traits including cell surface properties, mobility, osmotolerance, and growth rate have also been proposed to play a role in root colonization (13).…”

Section: Discussionmentioning

confidence: 99%

Effect of Two Plant Species, Flax (Linum usitatissinum L.) and Tomato (Lycopersicon esculentum Mill.), on the Diversity of Soilborne Populations of Fluorescent Pseudomonads

Lemanceau¹,

Corberand²,

Gardan³

et al. 1995

Appl Environ Microbiol

273

126

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Suppression of soilborne disease by fluorescent pseudomonads may be inconsistent. Inefficient root colonization by the introduced bacteria is often responsible for this inconsistency. To better understand the bacterial traits involved in root colonization, the effect of two plant species, flax (Linum usitatissinum L.) and tomato (Lycopersicon esculentum Mill.), on the diversity of soilborne populations was assessed. Fluorescent pseudomonads were isolated from an uncultivated soil and from rhizosphere, rhizoplane, and root tissue of flax and tomato cultivated in the same soil. Species and biovars were identified by classical biochemical and physiological tests. The ability of bacterial isolates to assimilate 147 different organic compounds and to show three different enzyme activities was assessed to determine their intraspecific phenotypic diversity. Numerical analysis of these characteristics allowed the clustering of isolates showing a high level (87.8%) of similarity. On the whole, the populations isolated from soil were different from those isolated from plants with respect to their phenotypic characteristics. The difference in bacteria isolated from uncultivated soil and from root tissue of flax was particularly marked. The intensity of plant selection was more strongly expressed with flax than with tomato plants. The selection was, at least partly, plant specific. The use of 10 different substrates allowed us to discriminate between flax and tomato isolates. Pseudomonas fluorescens biovars II, III, and V and Pseudomonas putida biovar A and intermediate type were well distributed among the isolates from soil, rhizosphere, and rhizoplane. Most isolates from root tissue of flax and tomato belonged to P. putida bv. A and to P. fluorescens bv. II, respectively. Phenotypic characterization of bacterial isolates was well correlated with genotypic characterization based on repetitive extragenic palindromic PCR fingerprinting.

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Section: Discussionmentioning

confidence: 99%

Effect of Two Plant Species, Flax (Linum usitatissinum L.) and Tomato (Lycopersicon esculentum Mill.), on the Diversity of Soilborne Populations of Fluorescent Pseudomonads

Lemanceau¹,

Corberand²,

Gardan³

et al. 1995

Appl Environ Microbiol

273

126

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“…Respiration and growth of R20(NAH7-1) in Moss Landing soil amended with salicylate. Microcosms containing 30 g of Moss Landing soil (Oceano loamy sand, pH 7.2) were prepared, incubated, and sampled for CO2 evolution and bacterial population densities as described elsewhere (3). Briefly, air-dried soil was moistened to -20 kPa with sterile distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…Strain PpG7 utilizes salicylate because it contains plasmid NAH7 which encodes the enzymes for naphthalene and salicylate degradation (6). Other research with Pseudomonas putida PpG7 showed that it responded to the incorporation of salicylate in several soil types by increased respiration and growth (3,4). However, examination of the quantitative relationships between population densities of strain PpG7 and its activity over many time periods showed a poor correlation between population density and activity (3).…”

mentioning

confidence: 99%

Enhanced Growth and Activity of a Biocontrol Bacterium Genetically Engineered To Utilize Salicylate

Colbert

Hendson

Ferri

et al. 1993

Appl Environ Microbiol

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Plasmid NAH7 was transferred from Pseudomonas putida PpG7 to P. putida R20 [R20(NAH7)], an antagonist of Pythium ultimum. The plasmid did not affect growth or survival of R20(NAH7) and was stably maintained under nonselective conditions in broth and soil and on sugar beet seeds. Plasmid NAH7 conferred to R20(NAH7) the ability to utilize salicylate in culture, agricultural field soil, and on sugar beet seeds. The metabolic activity of R20(NAH7), but not the wild-type R20, was greatly increased in soil by amendment with salicylate (250 ;ag/g) as measured by induced respiration. Population densities of R20(NAH7) were also enhanced in salicylate-amended soil, increasing from approximately 1 x 10V CFU/g to approximately 3 x 108 CFU/g after 35 h of incubation. In contrast, population densities of R20(NAH7) in nonamended soil were approximately 3 x 106 CFU/g of soil after 35 h of incubation. The concentration of salicylate in soil affected the rate and extent of population increase by R20(NAH7). At 50 to 250 pg of salicylate per g of soil, population densities of R20(NAH7) increased to approximately 108 CFU/g of soil by 48 h of incubation, with the fastest increase at 100 ,ug/g. A lag phase of approximately 24 h occurred before the population density increased in the presence of salicylate at 500 ,ug/g; at 1,000 t.g/g, population densities of R20(NAH7) declined over the time period of the experiment. Population densities of R20(NAH7) on sugar beet seeds in soils amended with 100 ,ug of salicylate per g were not increased while ample carbon was present in the spermosphere. However, after carbon from the seed had been utilized, population densities of R20(NAH7) decreased significantly less (P = 0.005) on sugar beet seeds in soil amended with salicylate than in nonamended soil.

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“…More successful manipulations to favor desirable microbial populations must provide a highly selective advantage for the introduced microorganism; usually, this advantage involves nutritional favoritism or suppression of indigenous populations. Thus, salicylate was used as carbon source to selectively increase activity and growth of Pseudomonas putida PpG7 in a soil,21 and the detergents Igepal CO‐720 and dicotyl sulfosuccinate were used to enrich for plant growth‐promoting, detergent‐adapted populations of P. fluorescence ANP15 and P. aeruginsa 7NSK2 in maize rhizospheres 22…”

mentioning

confidence: 99%

Phytoremediation: the state of rhizosphere ‘engineering’ for accelerated rhizodegradation of xenobiotic contaminants

Dzantor

2007

J of Chemical Tech & Biotech

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Phytoremediation has emerged as the method of choice for cleaning up a broad range of environmental contaminants. One process through which plants render some xenobiotic organic contaminants innocuous in soil involves plant-microbe interactions in which root exudates stimulate entire microbial communities, or induce specific enzymes in competent individuals to cause enhanced rhizodegradation. For some contaminants these inherent processes can be slow; however, potentials exist for their improvement through rhizosphere manipulations. Although this requires a greater understanding than currently exists with respect to plant and microbe components and interactions involved in the biodegradation of xenobiotic contaminants, improved understanding is being achieved by advances in biochemical and molecular characterization, and visualization of rhizosphere phenomena. In combination with earlier knowledge of naturally-occurring plant-microbe interactions such as the opine concept, this new knowledge considerably improves the opportunities for manipulating rhizosphere interactions to greatly accelerate rhizodegradation for routine practical implementation in the field.

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Use of an Exotic Carbon Source To Selectively Increase Metabolic Activity and Growth of Pseudomonas putida in Soil

Abstract: Respiration and growth of Pseudomonas putida PpG7, containing catabolic plasmid NAH7, was determined in three agricultural field soils amended with the carbon source salicylate. The addition of salicylate to soil

Cited by 34 publications

References 19 publications

Effect of Two Plant Species, Flax (Linum usitatissinum L.) and Tomato (Lycopersicon esculentum Mill.), on the Diversity of Soilborne Populations of Fluorescent Pseudomonads

Effect of Two Plant Species, Flax (Linum usitatissinum L.) and Tomato (Lycopersicon esculentum Mill.), on the Diversity of Soilborne Populations of Fluorescent Pseudomonads

Enhanced Growth and Activity of a Biocontrol Bacterium Genetically Engineered To Utilize Salicylate

Phytoremediation: the state of rhizosphere ‘engineering’ for accelerated rhizodegradation of xenobiotic contaminants

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