Use of an Aqueous Micellar Mobile Phase for Separation of Phenols and Polynuclear Aromatic Hydrocarbons via HPLC

Armstrong, Daniel W.; Henry, Susan

doi:10.1080/01483918008060181

Cited by 209 publications

(32 citation statements)

References 4 publications

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“…In 1980, Armstrong and Henry [25] first demonstrated the usefulness of replacing organic modifiers in reversed-phase liquid chromatography with an aqueous micellar solution. Named micellar liquid chromatography (MLC), its popularity is demonstrated by the large number of published papers [26 -31].…”

Section: Introductionmentioning

confidence: 99%

Separation and Determination of Phenolic Antioxidants by HPLC with Surfactant/n-Propanol Mobile Phases

Aparicio

Andrés

Vera

2000

J. High Resol. Chromatogr.

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Section: Introductionmentioning

confidence: 99%

Separation and Determination of Phenolic Antioxidants by HPLC with Surfactant/n-Propanol Mobile Phases

Aparicio

Andrés

Vera

2000

J. High Resol. Chromatogr.

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“…Hydro-organic mobile phases, such as water-acetonitrile (H 2 O-ACN), are traditionally used [1 -3]. Micellar liquid chromatography (MLC) has been studied [4 -13] since it was first introduced by Armstrong in 1980 [14]. Surfactant molecules form micellar aggregates at a critical micelle concentration (cmc).…”

Section: Introductionmentioning

confidence: 99%

“…To date, an organic additive is usually added in micellar mobile phase to speed up the mass transfer [9,20,21,23,25,26], and almost all the MLC studies have been done with polar molecules or small nonpolar molecules [20,21,23,24,27], such as one-or two-ring PAHs. The MLC separation of large multi-ring PAHs is not well-studied [8,25].…”

Section: Introductionmentioning

confidence: 99%

Optimization of micellar liquid chromatographic separation of polycyclic aromatic hydrocarbons with the addition of second organic additive

Mao

McGill

Tucker³

2004

J of Separation Science

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The micellar liquid chromatographic (MLC) separations of polycyclic aromatic hydrocarbons (PAHs) were optimized for three micellar systems, cetyltrimethylammonium chloride (CTAC), dodecyltrimethylammonium chloride (DTAC), and sodium dodecylsulfate (SDS), with 1-pentanol as the only organic additive. A difference in the separation was observed between CTAC and SDS/DTAC. Under each optimized separation conditions, CTAC-modified mobile phase provides the least desirable separation, which is attributed to its longer carbon tail (C16 vs. C12). In addition to 1-pentanol, the main organic additive, a second organic additive (3% 1-propanol) in the micelle-modified mobile phase was found to enhance the resolution of PAH chromatographic peaks. However, the extent of the enhancement varies for the different micellar systems, with the greatest resolution improvement seen for CTAC, and little effect for shorter-tail SDS and DTAC. This study shows the potential use of second organic additive (1-propanol), to the main nonpolar additive (1-pentanol), in facilitating the MLC separation of larger nonpolar compounds.

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“…The use ofmicellar liquid phases has been regarded as a powerful alternative to other aqueous-organic mobile phases for the analysis of biosamples [10][11][12]. Advantages of MLC are the capability of simultaneous separation of ionic and non-ionic compounds, possibility of simultaneous enhancement of solvent strength and separation selectivity, reproducible and predictable retention behavior, possibility of direct on-column injection of hydrophobic samples, enhanced luminescence detectability, safety and cost [13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Determination of synthetic antioxidants in dairy products and dietetic supplements by micellar liquid chromatography with direct sample injection

2000

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Key WordsColumn liquid chromatography Micellar liquid chromatography Milk Dietetic supplements Synthetic antioxidants SummaryA simple and rapid HPLC method for the determination of synthetic antioxidants (propyl gallate, tert-butylhydroquinone, 2,4,5-trihydroxybutyrophenone, nordihydroguaiaretic acid, octyl gallate, 3-tert-butyl-4-hydroxyanisole and dodecyl gallate) in powdered and liquid milk, cream of milk and dietetic supplements is described. The samples are diluted or solved in a micellar solution, filtered and directly injected. The retention behavior of the antioxidants on a C18 column, with micellar mobile phases containing SDS (0.05-0.15 M), n-propanol (1-9 %, v/v) and 10 mM phosphate at pH 3, has been studied by using mathematical models. Retention is predicted with errors below 3 %. To optimize the mobile phase composition, a procedure which takes into account the position and shape of the peaks is applied. The optimized mobile phase, which contains 0.090 M SDS and 6.6 % n-propanol, allows the separation of six antioxidants in less than 13 rain. Calibration curves are linear (r > 0.9998) and the limits of detection range from 0.05 to 0.3 ng antioxidant, which correspond to concentrations well below the levels allowed in foods. Repeatabilities for standards containing 5 #g mL q ranged from 0.2 to 1.6%.

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Use of an Aqueous Micellar Mobile Phase for Separation of Phenols and Polynuclear Aromatic Hydrocarbons via HPLC

Cited by 209 publications

References 4 publications

Separation and Determination of Phenolic Antioxidants by HPLC with Surfactant/n-Propanol Mobile Phases

Separation and Determination of Phenolic Antioxidants by HPLC with Surfactant/n-Propanol Mobile Phases

Optimization of micellar liquid chromatographic separation of polycyclic aromatic hydrocarbons with the addition of second organic additive

Determination of synthetic antioxidants in dairy products and dietetic supplements by micellar liquid chromatography with direct sample injection

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