Use of amides as cryoprotectants in extenders for frozen sperm of tambaqui, Colossoma macropomum

Varela, Antônio Sérgio; Corcini, Carine Dahl; Gheller, Stela Mari Meneghello; Jardim, Rodrigo Desessards; Lucia, Thomaz; Streit, Danilo Pedro; Figueiredo, Mario Roberto Chim

doi:10.1016/j.theriogenology.2012.02.029

Cited by 61 publications

(33 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The thawing of the samples was carried out by immersing the straws in water bath at 45°C for 8 sec (Maria et al, 2011;Varela Junior et al, 2012a). After thawing, the semen samples were analyzed to obtain the qualitative and quantitative parameters, following the methodology described for fresh semen, and after that sperm functionality was analyzed.…”

Section: Evaluation Of Quantitative and Qualitative Parametersmentioning

confidence: 99%

Functional integrity of Colossoma macropomum (Cuvier, 1816) sperm cryopreserved with enriched extender solutions

et al. 2015

View full text Add to dashboard Cite

Cryoprotectant solutions are used to protect the sperm from alterations caused by the low temperature in the cryopreservation process. We evaluated the quality of Colossoma macropomum semen after freezing, using dimethyl sulfoxide (DMSO) as a cryoprotectant, combined with two extender solutions (T1 -Solution 1: Glucose 90.0 g/L, Sodium Citrate 6.0 g/L, EDTA 1.5 g/L, Sodium Bicarbonate 1.5 g/L, Potassium Chloride 0.8 g/L, Gentamycin Sulphate 0.2 g/L, and T2 -Solution 2: Glucose 90.0 g/L, ACP ® -104 10.0 g/L). Motility rate and motility time did not differ between T1 and T2 and were lower than fresh semen. The number of normal sperm was significantly different in treatments T1 (15.1%) and T2 (21.9%), and both showed a reduction in the percentage of normal sperm compared to fresh semen (57.4%). The values found for the rates of fertilization and hatching, mitochondrial functionality and sperm DNA, did not differ between the treatments (T1 and T2). Regarding membrane integrity, there was a higher percentage of spermatozoa with intact membranes in T1 (53.4%) than T2 (43.7%). The extender solutions, combined with 10% DMSO, maintained the sperm DNA intact in almost all the C. macropomum sperm cells, however there was a loss in their functionality.As soluções crioprotetoras são utilizadas para proteger os espermatozoides das alterações causadas por baixas temperaturas durante o processo de criopreservação. Avaliamos a qualidade do sêmen de Colossoma macropomum após o congelamento, utilizando dimetilsulfóxido (DMSO) como crioprotetor, combinado com duas soluções diluidoras (T1 -Solução 1: Glicose 90,0 g/L, Citrato de Sódio 6,0 g/L, EDTA 1,5 g/L, Bicarbonato de Sódio 1,5 g/L, Cloreto de Potássio 0,8 g/L, Sulfato de Gentamicina 0,2 g/L, e T2 -Solução 2: Glicose 90,0 g/L, ACP®-104 10,0 g/L). A taxa de motilidade (%) e o tempo de motilidade (s) não diferiram entre T1 e T2, porém foram mais baixos do que no sêmen fresco. O número de espermatozoides normais foi significativamente diferente nos tratamentos T1 (15,1%) e T2 (21,9%), e ambos mostraram uma redução na porcentagem de espermatozoides normais, comparado ao sêmen fresco (57,4%). Os valores encontrados para as taxas de fertilização e eclosão, funcionalidade mitocondrial e DNA do esperma, não diferiram entre os tratamentos (T1 e T2). Para a integridade da membrana, houve uma porcentagem mais elevada de espermatozóides com a membrana intacta em T1 (53,4%) do que T2 (43,7%). As soluções diluentes combinadas com DMSO a 10% preservaram o DNA espermático intacto em quase todas as células do sêmen de C. macropomum, mas houve perda na funcionalidade dos mesmos.

show abstract

Section: Evaluation Of Quantitative and Qualitative Parametersmentioning

confidence: 99%

Functional integrity of Colossoma macropomum (Cuvier, 1816) sperm cryopreserved with enriched extender solutions

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Fertilization rate was assessed after 8 h (i.e., the number of eggs with cell division per total number of eggs; n = 200). Hatching rate was calculated as the number of hatched embryos from all evaluated embryos (VARELA et al, 2012).…”

Section: Fertilization and Post-thawing Sperm Evaluationmentioning

confidence: 99%

“…Sperm that emitted a green fluorescence was classified as viable since their metabolic activity allowed the CFDA to accumulate in their cytoplasm. Sperm that emitted a red or red/green fluorescence were classified as nonviable (VARELA JUNIOR et al, 2012). The percentage of sperm membrane integrity was determined calculating the proportion of sperm emitting green fluorescence to the total number of sperm (green, red, or red/green fluorescence).…”

Section: Fertilization and Post-thawing Sperm Evaluationmentioning

confidence: 99%

“…And a,fter 5 min, we registered the observations without exceeding slide exposure for >1 min. Sperm emitting green fluorescence were considered to have intact DNA, whereas those emitting red or orange fluorescence were considered to have denatured DNA (VARELA JUNIOR et al, 2012). The rate of DNA integrity was determined by the proportion of sperm emitting green fluorescence to the total number of sperm (green, red, or orange fluorescence).…”

Section: Fertilization and Post-thawing Sperm Evaluationmentioning

confidence: 99%

“…Sperm with positive rhodamine staining (i.e., green fluorescence) were considered to have functional mitochondria. On the other hand, nonfunctional mitochondria were characterized by negative rhodamine staining (i.e., sperm with no fluorescence) (VARELA JUNIOR et al, 2012). Rate of mitochondrial functionality was determined by estimating the proportion of sperm emitting green fluorescence to the total sperm count (green or no fluorescence).…”

Section: Fertilization and Post-thawing Sperm Evaluationmentioning

confidence: 99%

See 2 more Smart Citations

O papel do dimetilsulfóxido na criopreservação de sêmen de Curimba (Prochilodus lineatus)

et al. 2015

View full text Add to dashboard Cite

Cryopreservation of Curimba semen (Prochilodus lineatus) is ecological and commercial importance. The objective of this study was to evaluate the effect of different concentrations (2, 5, 8 and 11%) of dimethyl sulfoxide (DMSO) diluted in Betsville Thawing Solution (BTS) on the quality of post-thaw semen Curimba. We analyzed the rate and period motility, sperm viability, membrane integrity and DNA, mitochondrial functionality, and fertilization and hatching rate. The plasma membrane and DNA integrity of a DMSO concentration of 11% obtained better results than the concentration of 5% (p <0.05). However, treatment of 5% DMSO resulted in a longer latency and a higher fertilization rate and hatching, in other sperm quality equal to that of fresh semen. The results of this study indicate that 5% DMSO is ideal for cryopreservation of semen Curimba. Key words: Freezing, fertilization, hatching, mitochondria, Prochilodus lineatus Resumo A criopreservação de sêmen de Curimba (Prochilodus lineatus) é de importância ecológica e comercial. O objetivo deste estudo foi avaliar o efeito de diferentes concentrações (2, 5, 8 e 11%) de dimetilsulfóxido (DMSO) diluído em Betsville Thawing Solution (BTS) sobre a qualidade do sêmen de Curimba pós-descongelamento. Foram analisadas a taxa e período de motilidade, viabilidade espermática, integridade da membrana plasmática e do DNA, funcionalidade mitocondrial, e taxa de fertilização e eclosão. A membrana plasmática e a integridade do DNA a uma concentração de DMSO de 11% obtiveram melhores resultados que na concentração de 5% (p <0,05). Contudo, o tratamento de 5% de DMSO resultou em um aumento da latência e uma maior taxa de fertilização e de eclosão, com as demais qualidades do esperma sendo semelhante ao de sêmen fresco. Os resultados deste estudo indicam que 5% de DMSO é ideal para a criopreservação do sêmen Curimba.

show abstract

Roundup® Herbicide Decreases Quality Parameters of Spermatozoa of Silversides Odontesthes Humensis

Silveira

Varela

Corcini

et al. 2018

Bull Environ Contam Toxicol

View full text Add to dashboard Cite

Use of amides as cryoprotectants in extenders for frozen sperm of tambaqui, Colossoma macropomum

Cited by 61 publications

References 48 publications

Functional integrity of Colossoma macropomum (Cuvier, 1816) sperm cryopreserved with enriched extender solutions

Functional integrity of Colossoma macropomum (Cuvier, 1816) sperm cryopreserved with enriched extender solutions

O papel do dimetilsulfóxido na criopreservação de sêmen de Curimba (Prochilodus lineatus)

Roundup® Herbicide Decreases Quality Parameters of Spermatozoa of Silversides Odontesthes Humensis

Contact Info

Product

Resources

About