Use of allyl isothiocyanate and carbon nanotubes in an antimicrobial film to package shredded, cooked chicken meat

Dias, Marali Vilela; Soares, Nilda de Fátima Ferreira; Borges, Soraia Vilela; Sousa, Miriane Maria de; Nunes, Cleiton Antônio; Oliveira, Isadora Rebouças Nolasco de; Medeiros, Éber Antônio Alves

doi:10.1016/j.foodchem.2013.05.148

Cited by 89 publications

(39 citation statements)

References 31 publications

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“…Allyl isothiocyanate has been tested as a preservative for a wide variety of foods, including water (36), spinach leaves (37), cherry tomatoes (38), fresh cut onions (39), cantaloupe (40), kimchi (41), bread (42), chicken meat (43,44), catfish (45), and ham (46). The application of allyl isothiocyanate is usually via microencapsulation into different matrices to trap the Comparative genome analysis of pectobacteria for saxA genes.…”

Section: Resultsmentioning

confidence: 99%

SaxA-Mediated Isothiocyanate Metabolism in Phytopathogenic Pectobacteria

Welte

Rosengarten

Graaf

et al. 2016

Appl Environ Microbiol

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Pectobacteria are devastating plant pathogens that infect a large variety of crops, including members of the family Brassicaceae. To infect cabbage crops, these plant pathogens need to overcome the plant's antibacterial defense mechanisms, where isothiocyanates are liberated by hydrolysis of glucosinolates. Here, we found that a Pectobacterium isolate from the gut of cabbage root fly larvae was particularly resistant to isothiocyanate and even seemed to benefit from the abundant Brassica root metabolite 2-phenylethyl isothiocyanate as a nitrogen source in an ecosystem where nitrogen is scarce. The Pectobacterium isolate harbored a naturally occurring mobile plasmid that contained a sax operon. We hypothesized that SaxA was the enzyme responsible for the breakdown of 2-phenylethyl isothiocyanate. Subsequently, we heterologously produced and purified the SaxA protein and characterized the recombinant enzyme. It hydrolyzed 2-phenylethyl isothiocyanate to yield the products carbonyl sulfide and phenylethylamine. It was also active toward another aromatic isothiocyanate but hardly toward aliphatic isothiocyanates. It belongs to the class B metal-dependent beta-lactamase fold protein family but was not, however, able to hydrolyze beta-lactam antibiotics. We discovered that several copies of the saxA gene are widespread in full and draft Pectobacterium genomes and therefore hypothesize that SaxA might be a new pathogenicity factor of the genus Pectobacterium, possibly compromising food preservation strategies using isothiocyanates. P ectobacteria are phytopathogens that cause tuber soft rot and blackleg (stem rot) in many horticulturally and economically important crops during cultivation, transport, or storage. Most Pectobacterium spp., e.g., Pectobacterium carotovorum or P. wasabiae, can be detrimental to many different plants, such as potato, sugar beet, cabbage, wasabi, chicory, and Ornithogalum plants, whereas other Pectobacterium spp., e.g., P. atrosepticum and P. betavasulorum, have a more narrow host range of potato and sugar beet plants, respectively (1).Several pathogenicity factors of soft-rot-causing Pectobacterium spp. have been found during the last decades (2-4). Of these, plant cell wall-degrading enzymes produced by Pectobacterium spp. have a large impact on the progress of the disease, as their production leads to the degradation of invaded plant tissue (2, 3). The production of these enzymes is dependent on cell density and regulated by quorum sensing through N-acetylhomoserine lactone (5) and intracellular regulators (6, 7). Motility and nutrient uptake are also factors influencing phytopathogenicity (4,(8)(9)(10). Besides these general pathogenicity factors, phytopathogens have to overcome the toxicity of plant allelochemicals. Important allelochemicals of members of the family Brassicaceae (cabbages and mustards) are isothiocyanates that are liberated by the glucosinolate-myrosinase defense system (11-13). The antimicrobial effect of isothiocyanates has been mainly attributed to their reactivity w...

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Section: Resultsmentioning

confidence: 99%

SaxA-Mediated Isothiocyanate Metabolism in Phytopathogenic Pectobacteria

Welte

Rosengarten

Graaf

et al. 2016

Appl Environ Microbiol

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“…Especially isothiocyanates are toxic to most herbivorous insects that are one of the prime targets of this chemical defence mechanism of plants (Hopkins et al ., ). Isothiocyanates are antimicrobial compounds and are already tested to be used as food preservatives to prevent bacterial food spoilage (Dias et al ., ; Jideani and Vogt, ). In view of the apparent resistance of D. radicum larvae to the glucosinolate defence system, we studied the possible involvement of their gut‐associated microbes in the degradation of the Brassica root volatile 2‐phenylethyl isothiocyanate.…”

Section: Introductionmentioning

confidence: 99%

Plasmids from the gut microbiome of cabbage root fly larvae encode SaxA that catalyses the conversion of the plant toxin 2‐phenylethyl isothiocyanate

Welte

Graaf

Bosch

et al. 2015

Environmental Microbiology

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Cabbage root fly larvae (Delia radicum) cause severe crop losses (≥ 50%) of rapeseed/ canola and cabbages used in the food and biofuel industries. These losses occur despite the fact that cabbages produce insecticidal toxins such as isothiocyanates. Here we describe the cabbage root fly larval gut microbiome as a source of isothiocyanate degrading enzymes. We sequenced the microbial gut community of the larvae and analysed phylogenetic markers and functional genes. We combined this with the isolation of several microbial strains representing the phylogenetic distribution of the metagenome. Eleven of those isolates were highly resistant towards 2-phenylethyl isothiocyanate, a subset also metabolized 2-phenylethyl isothiocyanate. Several plasmids appeared to be shared between those isolates that metabolized the toxin. One of the plasmids harboured a saxA gene that upon transformation gave resistance and enabled the degradation of 2-phenylethyl isothiocyanate in Escherichia coli. Taken together, the results showed that the cabbage root fly larval gut microbiome is capable of isothiocyanate degradation, a characteristic that has not been observed before, and may help us understand and design new pest control strategies.

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“…Thiobarbituric acid reactive substances (TBARS) were determined according to Dias et al A sample of 5 g was mixed with 25 mL of 7.5% trichloroacetic acid (TCA) in water to extract malondialdehyde (MDA). Samples were homogenized in Ultra Turrax for 1 minute and filtered with funnel and filter paper.…”

Section: Methodsmentioning

confidence: 99%

“…To quantify tocopherol, a standard curve with different known concentrations of the substance was obtained with retention time of 9.2 minutes. The quantification limit of the equipment for α-tocopherol was determined as being 0.6 μg/mL.2.7.2 | Measurement of lipid oxidationThiobarbituric acid reactive substances (TBARS) were determined according to Dias et al25 A sample of 5 g was mixed with 25 mL of 7.5% trichloroacetic acid (TCA) in water to extract malondialdehyde (MDA). Samples were homogenized in Ultra Turrax for 1 minute and filtered with funnel and filter paper.…”

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confidence: 99%

Effect of active films incorporated with montmorillonite clay and α‐tocopherol: Potential of nanoparticle migration and reduction of lipid oxidation in salmon

et al. 2018

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Fat‐rich fish such as salmon are highly susceptible to lipid oxidation, which affects the quality of the product. The compound α‐tocopherol is widely used as an antioxidant added directly to food or through the incorporation in active packaging besides having activity of vitamin E, which is an essential nutrient to the human body absorbed from food. The objective of this paper was to evaluate the montmorillonite (MMT15A) and α‐tocopherol migration potential and antioxidant effect of chitosan/MMT15A/α‐tocopherol active films on reduction of lipid oxidation in fresh salmon. Chitosan films incorporated with MMT15A (0 and 1 g/100 g) and α‐tocopherol (0, 10, and 15 g/100 g) were applied on the fresh salmon for 8 days. Samples of salmon wrapped in films 3 and 6 (10% tocopherol and 1% MMT15A + 15%tocopherol, respectively) showed the lower values of thiobarbituric acid reactive substances (TBARS) at eighth storage day at 4°C. Film 6 (1% MMT15A and 15% tocopherol) showed better result on reduction in water vapour transmission rate (WVTR) and controlled release of tocopherol. All the films containing MMT15A presented migration of minerals (Mg and Si) for the salmon during the 8 days. Thus, the use of this film for release of active substances is a promising alternative for application as food packaging in order to obtain nutritionally fortified foods and inhibit oxidation reactions.

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Use of allyl isothiocyanate and carbon nanotubes in an antimicrobial film to package shredded, cooked chicken meat

Cited by 89 publications

References 31 publications

SaxA-Mediated Isothiocyanate Metabolism in Phytopathogenic Pectobacteria

SaxA-Mediated Isothiocyanate Metabolism in Phytopathogenic Pectobacteria

Plasmids from the gut microbiome of cabbage root fly larvae encode SaxA that catalyses the conversion of the plant toxin 2‐phenylethyl isothiocyanate

Effect of active films incorporated with montmorillonite clay and α‐tocopherol: Potential of nanoparticle migration and reduction of lipid oxidation in salmon

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