Use of adenoviral vectors as veterinary vaccines

Ferreira, Tiago Borges; Alves, Paula M.; Auniņš, John G.; Carrondo, Manuel J.T.

doi:10.1038/sj.gt.3302618

Cited by 69 publications

(34 citation statements)

References 89 publications

(101 reference statements)

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“…27,28 In this study, AdEasy-1 system has been selected for generation of recombinant adenoviruses encoding hNET gene. Compared with traditional adenoviral vectors, the AdEasy-1 system incorporates several unusual features.…”

Section: Discussionmentioning

confidence: 99%

“…Second, the recombination step is performed in Escherichia coli rather than in mammalian cells, taking advantage of the high efficiency of homologous recombination in bacteria. 28 Furthermore, there is no need to carry out laborious plaque purification rounds, which reduce the needed time to generate a recombinant adenovirus, to several weeks.…”

Section: Discussionmentioning

confidence: 99%

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In vitro and in vivo studies of adenovirus-mediated human norepinephrine transporter gene transduction to hepatocellular carcinoma

Huang

et al. 2010

Cancer Gene Ther

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The clinical value of 131 I-MIBG for targeted imaging and targeted radiotherapy is limited to neural crest-derived tumors expressing human norepinephrine transporters (hNET) protein. To extend 131 I-MIBG-targeted therapy to other nonexpressed hNET tumors, this study investigated the hNET expression in vitro and in vivo in HepG2 hepatoma mediated by recombinant adenovirus encoding the hNET gene (Ad-hNET). For this purpose, the HepG2 cells showed a 4.87-fold increase in 125 I-MIBG uptake after infection with Ad-hNET, and the uptake of 125 I-MIBG could be specifically inhibited by maprotiline. Immunohistological analysis, in vivo biological study and 131 I-MIBG scintigraphic imaging also revealed the high expression of hNET protein in hepatoma. This in vitro and in vivo studies demonstrate the feasibility of hNET gene transfer, meditated by adenovirus vector, could extend to tumors other than those derived from the neural crest, which provides a sound foundation for further investigation of hepatocellular carcinomatargeted radiotherapy mediated by adenovirus transfection with hNET gene.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo studies of adenovirus-mediated human norepinephrine transporter gene transduction to hepatocellular carcinoma

Huang

et al. 2010

Cancer Gene Ther

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“…The genome of non integrating viral vectors, such as adenoviruses, will get lost after several cell divisions, but these viruses have been shown to be useful in gene therapy approaches in cancer research (Gallo et al, 2005), or new developments of vaccines in veterinary medicine (Ferreira et al, 2005). …”

Section: Vector Designmentioning

confidence: 99%

Evaluation of laser-assisted lentiviral transgenesis in bovine

et al. 2006

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“…Gene therapy is increasingly viewed as a novel form of drug therapy and a source of new therapeutic products for pharmaceutical companies [1,2]. Adenoviral vectors are currently the most utilized viral vectors in gene therapy industry [3].…”

Section: Introductionmentioning

confidence: 99%

Effect of Pluronic F-68, 5% CO, Atmosphere, HEPES, and Antibiotic-Antimycotic on Suspension Adapted 293 Cells

Negrete¹,

Ling²,

Lyddiatt³

2008

TOBIOTJ

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Abstract:The influence of different parameters upon cell culture of serum-free adapted 293 cells including the surfactant Pluronic F-68, carbon dioxide (5% CO 2 atmosphere), buffer HEPES and antibiotic-antimycotic has been explored. A defined serum-free medium (SFM) formulated without human or animal origin components from Invitrogen was used to grow the suspension adapted 293 cells. For all cell culture parameters cell density and viability of the suspension adapted 293 cells were monitored. The results indicated that the PF68 concentrations ranging from 0.05% to 0.2% can be used in the culture of the suspension adapted 293 cells since no negative effect upon either cell density or viability was detected. This will minimize the formation of aggregates during cell culture. It was demonstrated that neither the cell density nor the viability of the suspension adapted 293 cells were affected by the 5% CO 2 atmosphere at the inoculation cell densities evaluated. The use of the buffer HEPES in the cultivation of suspension adapted 293 cells did not cause negative effects upon cell density and viability. The addition of HEPES makes more robust the culture to pH fluctuations. The antibioticantimycotic can be used when needed at concentrations of up to 50 IU/ml for the culture of this particular cell line, with no apparent effect upon cell growth. The results obtained will contribute to a basic understanding of the 293 cell culture in the 293 SFM II and to the process development of their culture in bioreactors for the expression of different products of biotechnology interest.

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Use of adenoviral vectors as veterinary vaccines

Cited by 69 publications

References 89 publications

In vitro and in vivo studies of adenovirus-mediated human norepinephrine transporter gene transduction to hepatocellular carcinoma

In vitro and in vivo studies of adenovirus-mediated human norepinephrine transporter gene transduction to hepatocellular carcinoma

Evaluation of laser-assisted lentiviral transgenesis in bovine

Effect of Pluronic F-68, 5% CO, Atmosphere, HEPES, and Antibiotic-Antimycotic on Suspension Adapted 293 Cells

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