Use of Acadian marine plant extract powder from Ascophyllum nodosum in tissue culture of Kappaphycus varieties

Hurtado, Anicia Q.; Yunque, Dianne Aster T.; Tibubos, Keneth R.; Critchley, Alan T.

doi:10.1007/s10811-008-9395-4

Cited by 89 publications

(40 citation statements)

References 23 publications

(31 reference statements)

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“…Successful culture of micropropagules of K. alvarezii was already done in the laboratory (Dawes and Koch 1991;Dawes et al 1993Dawes et al , 1994. Smaller sections of seaweeds were used for tissue culture and showed some promise (Muñoz et al, 2006;Hurtado and Biter 2007;Hurtado et al 2009;Hayashi et al 2008). Tetrasporophyte progenies have been shed in the laboratory and grown outdoors (Bulboa et al 2007(Bulboa et al , 2008Bulboa and De Paula 2005).…”

Section: Introductionmentioning

confidence: 99%

Growing the reproductive cells (carpospores) of the seaweed, Kappaphycus striatum, in the laboratory until outplanting in the field and maturation to tetrasporophyte

Luhan

Sollesta

2010

J Appl Phycol

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Carposporophytes of the seaweed, Kappaphycus striatum, from the wild were made to shed spores in the laboratory and grown in multi-step culture method until they reached maturity. For each succeeding transfer onto increasingly bigger culture vessels, there was a marked increase in the growth of carposporelings. When plantlets were ready for outdoor culture, they were placed in aquaria and concrete tanks and later moved to the sea in net cage and long-line for grow-out culture. Successfully growing sporelings from carposporophytes in the laboratory until they reach market size seems to depend on the stage of sporelings and environmental factors such as photoperiod and temperature. In this study, carpospore progenies (diploids) also matured into tetrasporophytes and haploid progenies showed resistance to higher temperature.

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Section: Introductionmentioning

confidence: 99%

Growing the reproductive cells (carpospores) of the seaweed, Kappaphycus striatum, in the laboratory until outplanting in the field and maturation to tetrasporophyte

Luhan

Sollesta

2010

J Appl Phycol

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“…The collection sites have grown the explants commercially recently along the coast of the Zamboanga Peninsula. The plants were placed in an ice chest lined with seawater-moistened cheesecloth and transported to the Aquaculture Department of the Southeast Asian Fisheries Development Center (SEAFDEC/AQD) Tigbauan, Iloilo, Philippines, following the protocol described by Hurtado et al (2009). The explants were acclimatized in concrete tanks with continuous aeration and a flow-through seawater system for 7 days.…”

Section: Methodsmentioning

confidence: 99%

“…The application of AMPEP has been extended to laboratory tissue culture techniques for the regeneration of the carrageenophyte Kappaphycus alvarezii varieties for nursery purposes in the Philippines (Hurtado et al 2009). Loureiro et al (2009) also reported on a substantial increase in the growth rates of K.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, AMPEP is 100% water soluble and does not require further addition of expensive reagents which can be complicated to prepare, thereby making it a potentially suitable medium for the large-scale production of carrageenophytes in the major Southeast Asian areas of commercial carrageenophyte production (Hurtado et al 2009). This study was conducted to determine the optimal AMPEP concentration, pH, temperature, and density of explants for the commercial production of K. alvarezii varieties using tissue culture techniques.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of culture conditions for tissue culture production of young plantlets of carrageenophyte Kappaphycus

et al. 2010

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To improve the production of Kappaphycus plantlets in tissue culture, optimum media concentrations of an Ascophyllum nodosum extract (Acadian Marine Plant Extract Powder, AMPEP), plant growth regulators (PGR), pHtemperature combinations, and explant density were determined. Kappaphycus alvarezii var. tambalang purple (PUR), kapilaran brown (KAP), vanguard brown (VAN), adik-adik (AA), tungawan green (TGR), and K. striatum var. sacol green (GS) were used as explants. Based on the shortest period for shoot emergence and the economical use of AMPEP, the optimum enriched media was 3.0 mg L −1 AMPEP and 0.1 mg L −1 AMPEP+PGR 1 mg L −1 each phenylacetic acid (PAA) and zeatin for PUR, 1.0 mg L −1 AMPEP+PGR for KAP and GS, 0.1 mg L −1 AMPEP+PGR for VAN, and 3.0 mg L −1 AMPEP and 0.001 mg L −1 AMPEP+PGR for AA and TGR. Results showed that the addition of PGR to low concentrations of AMPEP hastened shoot formation. pH-temperature combinations for the most rapid shoot formation were determined for the brown (KAP) and purple (PUR) color morphotypes of K. alvarezii var. tambalang and the green morphotype of K. striatum var.sacol (GS) cultured in 1.0 mg L −1 AMPEP+PGR. The brown morphotype produced the most number of shoots at pH 7.7 at 20°C after as little as 20 days. Purple K. alvarezii showed an increased shoot formation at pH 6.7 at 25°C and the green K. striatum morphotype at pH 8.7 at 25°C. The optimum number of explants added to the culture media was also determined for tungawan green (TGR), brown (KAP), and tambalang purple (PUR) varieties of K. alvarezii in 1.0 mg L −1 AMPEP+PGR. The number of explants and the volume of the culture media combination were also tested. The highest average number of shoots formed occurred in two explants:1 mL culture media (2:1) for KAP and PUR (35.00% and 16.67%, respectively) and 1 explant: 2 mL culture media for the TGR (100.00%) with a range of 0.5-3.0 mm shoot length after 40 days in culture. The earliest shoot formation was observed after 21 days for the brown and 9 days for both the green and purple color morphotypes of Kappaphycus, in all densities investigated. This indicated that within the range tested, the density of explants did not have a significant effect on the rate of shoot formation but did influence the average number generated from the culture. The rate of production of new and improved Kappaphycus explants for a commercial nursery stock was improved through the use of AMPEP with optimized culture media pH, temperature, and density conditions.

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“…Acadian marine plant extract powder (AMPEP) is another alga powder that can improve callus growth. This alga powder is obtained from fresh Ascophyllum nodosum and contains the major and minor nutrients, carbohydrates, amino acids and plant growth promoting substances which are required for callus growth 39 .…”

Section: −1mentioning

confidence: 99%

Novel medium for enhancing callus growth in hazel (Corylus avellana L.)

Moieni¹,

Salehi²,

Safaie³

2017

J Plant Physiol Pathol

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Paclitaxel is a powerful antimitotic agent with excellent activity against a range of cancers. Hazel has been described as a paclitaxel-producing species among angiosperms. Fast-growing callus is a prerequisite for the success of callus production and then paclitaxel production. Therefore, optimizing the medium culture for enhancing callus growth is a crucial step for paclitaxel production. In this research, Murashige and Skoog (1962) (MS) medium was optimized for improving callus growth of hazel (Corylus avellana L.). The M 10 medium (MS medium with pH 6.0 and supplemented with 1000 mg l −1 spirulina powder, 1000 mg l −1 casein hydrolysate and 3 g l −1 gelrite) significantly improved hazel callus growth. This modified MS medium increased callus fresh weight (55.8%) as compared to the control. M 10 medium increased fatty acids yield of callus (66.7%) as compared to the control. Liquid M 10 medium maintained growth over a longer period of time and also increased slightly, the paclitaxel production as compared to the control. This novel medium is promising for facilitating the mass production of hazel callus as a source of valuable metabolites including paclitaxel, linoleic and oleic acids.Paclitaxel is a powerful antimitotic agent with excellent activity against a range of cancers 1 . The major limitation in the extensive use of this valuable secondary metabolite is its low supply, since Taxus spp. contains very low amounts of paclitaxel 2 . Extraction of paclitaxel from this tree has imposed important ecological effects, resulting in the extinction of Taxus species 3 . Plant cell suspension culture is considered as the most promising approach to the production of paclitaxel 4 . The availability of this drug is still restricted and its cost is very high, mainly due to the recalcitrant behavior of Taxus spp. under in vitro conditions 2 . Therefore, the search for alternative sources of paclitaxel was considered as crucial. In addition to Taxus spp., hazel (Corylus avellana) has also been described as a paclitaxel-producing species through bioprospection among angiosperms 5,6 . The major advantages of producing paclitaxel through hazel cell cultures are that hazel is widely accessible and its in vitro cultivation is easier than that of yew 2,7 . It is stated that in vitro cultures of C. avellana can become a promising and cheaper source for paclitaxel production 8 . Besides the use of the nuts of hazel tree as a source of protein, its leaves are used to relieve the symptoms of hemorrhoidal and varicose veins 9 . The kernel and green leaf/flower portions of the hazel tree have antioxidant activity 10 . It is found that the consumption of nuts is protective against cardiac morbidity and mortality 11. In addition to the use of hazel cell cultures for paclitaxel production, hazel plantlets can be regenerated from callus tissues by differentiation induced by exogenous growth regulators. Plant regeneration from calli is possible by somatic embryogenesis or in vitro organogenesis. Meanwhile, infrequent somaclonal variants...

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Use of Acadian marine plant extract powder from Ascophyllum nodosum in tissue culture of Kappaphycus varieties

Cited by 89 publications

References 23 publications

Growing the reproductive cells (carpospores) of the seaweed, Kappaphycus striatum, in the laboratory until outplanting in the field and maturation to tetrasporophyte

Growing the reproductive cells (carpospores) of the seaweed, Kappaphycus striatum, in the laboratory until outplanting in the field and maturation to tetrasporophyte

Optimization of culture conditions for tissue culture production of young plantlets of carrageenophyte Kappaphycus

Novel medium for enhancing callus growth in hazel (Corylus avellana L.)

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