Use of a Systematic Pharmacological Methodology to Explore the Mechanism of Shengmai Powder in Treating Diabetic Cardiomyopathy

Zhang, Shiying; Yang, Kailin; Long, Zhiyong; Li, Weiqing; Huang, Huansen

doi:10.12659/msm.919029

Cited by 5 publications

(6 citation statements)

References 81 publications

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“…Set the confidence to 0.700 (A higher score gives a higher degree of confidence in the protein interaction), and P value <1.0 e–16. The obtained protein interaction information was imported into Cytoscape 3.7.0, and the topological network was analyzed by using Network Analyzer [13,14] …”

Section: Methodsmentioning

confidence: 99%

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Ellagic Acid Alleviates Mice Intestinal Ischemia‐Reperfusion Injury: A Study Based on Transcriptomics Combined with Functional Experiments

yuan

et al. 2022

Chemistry & Biodiversity

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BackgroundIt has been reported that intestinal ischemia-reperfusion injury (IIRI) is closely related to in ammatory response, apoptosis and oxidative stress. Ellagic acid (EA) has been proved to have antioxidant and antiin ammatory effects, and can inhibit tumor angiogenesis. The purpose of this study was to investigate the protective effects of EA on IIRI in mice. MethodsA mouse model of IIRI was established by clamping the mesenteric artery. Effects and mechanisms of EA on IIRI were investigate by transcriptomics combined with functional experiments. ResultsThe symptoms of IIRI were re ected in signi cant increases in in ammatory factors such as TNF-α and IL-1β; signi cant increases in oxidative stress indicators such as MDA and GSH and decreases in SOD and promotion of the apoptotic protein Bax/Bcl-2. These indicators were signi cantly alleviated by EA.And after EA treatment, transcriptomics results identi ed AKT1 differentially expressed mRNAs mainly enriched in PI3K/AKT signalling pathway. ConclusionThis study illustrates the protective effects against IIRI, the possible mechanisms were also studied. This study provides new scienti c information for the application of EA in IIRI therapy.

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Section: Methodsmentioning

confidence: 99%

“…The obtained protein interaction information was imported into Cytoscape 3.7.0, and the topological network was analyzed by using Network Analyzer. [13,14]…”

Section: Construction Of the Ea Target Ppi Networkmentioning

confidence: 99%

Ellagic Acid Alleviates Mice Intestinal Ischemia‐Reperfusion Injury: A Study Based on Transcriptomics Combined with Functional Experiments

yuan

et al. 2022

Chemistry & Biodiversity

View full text Add to dashboard Cite

show abstract

“…Set the con dence to 0.700 (A higher score gives a higher degree of con dence in the protein interaction), and P value < 1.0e-16. The obtained protein interaction information was imported into Cytoscape 3.7.0, and the topological network was analyzed by using Network Analyzer (Jiang ZY 2021, Zhang et al 2020) .…”

Section: Construction Of the Ea Target Ppi Networkmentioning

confidence: 99%

Ellagic Acid Alleviates Mice intestinal ischemia-reperfusion injury: A Study Based on Transcriptomics Combined with Functional Experiments

Fan

et al. 2022

Preprint

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Background：It has been reported that intestinal ischemia-reperfusion injury (IIRI) is closely related to inflammatory response, apoptosis and oxidative stress. Ellagic acid (EA) has been proved to have antioxidant and anti-inflammatory effects, and can inhibit tumor angiogenesis. The purpose of this study was to investigate the protective effects of EA on IIRI in mice.Methods：A mouse model of IIRI was established by clamping the mesenteric artery. Effects and mechanisms of EA on IIRI were investigate by transcriptomics combined with functional experiments. Results: The symptoms of IIRI were reflected in significant increases in inflammatory factors such as TNF-α and IL-1β; significant increases in oxidative stress indicators such as MDA and GSH and decreases in SOD and promotion of the apoptotic protein Bax/Bcl-2. These indicators were significantly alleviated by EA. And after EA treatment, transcriptomics results identified AKT1 differentially expressed mRNAs mainly enriched in PI3K/AKT signalling pathway. Conclusion: This study illustrates the protective effects against IIRI, the possible mechanisms were also studied. This study provides new scientific information for the application of EA in IIRI therapy.

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“… 6–8 Using a systematic pharmacological methodology, Zhang et al found that SMS could regulate various signaling pathways and biological processes related to glucose and lipid metabolism, inflammation, and oxidative stress in the myocardium of diabetic rats, thus possessing a therapeutic value for DCM. 9 However, the molecular mechanism of SMS in DCM remains unclear.…”

Section: Introductionmentioning

confidence: 99%

ShengMai-San Attenuates Cardiac Remodeling in Diabetic Rats by Inhibiting NOX-Mediated Oxidative Stress

Lu¹,

Zhu²,

Wang³

et al. 2021

DMSO

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Background and Purpose ShengMai-San (SMS) is traditionally used to treat ischemic cardiovascular and cerebrovascular diseases. Recently, several studies have reported the cardioprotective effects of SMS in diabetic animals. However, the potential mechanisms have not yet been fully elucidated. In this study, we investigated whether SMS exerts a beneficial effect in diabetic cardiomyopathy (DCM) by alleviating NADPH oxidase (NOX)-mediated oxidative stress. Methods SD rats were randomly divided into a negative control group (NC), diabetes mellitus group (DM) and SMS-treated group (SMS). The myocardial structure alterations, apoptosis and biomarkers of oxidative stress were observed. Moreover, to explore the protective mechanism of SMS, the activation of AMPKα, expression and translocation of NOX-related proteins were assessed. Results Diabetes led to excessive collagen content, fibrosis, and apoptosis in the myocardium. Oxidative stress in diabetic hearts was indicated by low levels of T-AOC, high levels of 8-iso-PGF2α and 8-OHdG, inactivation of AMPKα, elevated expression of NOX2 and NOX4 and translocation of NOX isoforms p47phox and p67phox. Treatment with SMS for 10 weeks resulted in the alleviation of diabetes-associated myocardial structure abnormalities and apoptosis. Additionally, SMS attenuated the accumulation of oxidative stress markers in myocardial tissue. Further investigation showed that SMS was able to reverse the levels of oxidative stress-associated proteins NOX2 and NOX4 in the DM rats. Moreover, SMS treatment blunted the translocation of NADPH oxidase isoforms p47phox and p67phox as well. Furthermore, SMS promoted the activation of AMPK in the cardiac tissue of diabetic rats. Conclusion These findings indicate that SMS exhibits therapeutic properties against diabetic cardiomyopathy by attenuating myocardial oxidative damage via activation of AMPKα and inhibition of NOX signaling.

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Use of a Systematic Pharmacological Methodology to Explore the Mechanism of Shengmai Powder in Treating Diabetic Cardiomyopathy

Cited by 5 publications

References 81 publications

Ellagic Acid Alleviates Mice Intestinal Ischemia‐Reperfusion Injury: A Study Based on Transcriptomics Combined with Functional Experiments

Ellagic Acid Alleviates Mice Intestinal Ischemia‐Reperfusion Injury: A Study Based on Transcriptomics Combined with Functional Experiments

Ellagic Acid Alleviates Mice intestinal ischemia-reperfusion injury: A Study Based on Transcriptomics Combined with Functional Experiments

ShengMai-San Attenuates Cardiac Remodeling in Diabetic Rats by Inhibiting NOX-Mediated Oxidative Stress

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