Use of a SPAD-502 meter to measure leaf chlorophyll concentration in Arabidopsis thaliana

Ling, Qihua; Huang, Weihua; Jarvis, Paul

doi:10.1007/s11120-010-9606-0

Cited by 353 publications

(233 citation statements)

References 25 publications

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“…The SPAD-502 meter measures leaf transmittance in the red (650 nm) and infrared (940 nm) bands. The SPAD meter value, typically between 0.0 and 50.0, is proportional to the amount of chlorophyll in the leaf [24]. Both NDVI and SPAD readings were measured and recorded every second week.…”

Section: Growth and Physiological Measurementsmentioning

confidence: 99%

Reduction in Water Stress for Tree Saplings Using Hydrogels in Soil

Kargar¹,

Suresh²,

Legrand³

et al. 2017

GEP

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The effect of soil amendment with hydrogel on reducing water stress was tested for Siberian elm (Ulmus pumila) and silver maple (Acer saccharinum) saplings. The trees were planted in soils with one of two concentrations of hydrogel (0.5% or 1% dry weight) as compared to the control soil (0% of hydrogel) and watered either daily, weekly, or bi-weekly. Growth was monitored by measuring height and stem diameter. Stress was monitored by measuring SPAD readings and normalized difference vegetation index (NDVI), as proxy measures of chlorophyll content and photosynthetic activity, respectively. Water stress decreased NDVI (p < 0.05) but did not have a significant effect on SPAD readings. Soil with 0.5% concentration of hydrogel was positively associated with greater height and NDVI (p < 0.01) for both maple and elm trees. Hydrogels had a species-specific effect on SPAD readings. The interaction between hydrogel concentration and the watering regime had a significant effect on the height and NDVI (p < 0.01) of elms, but not maples. The improved performance of water-stressed tree saplings in hydrogel-amended soils was presumably due to the ability of hydrogels to absorb and then gradually release water and nutrients. This is of special interest for urban foresters, because water stress and nutrient deficiency are two important growthlimiting factors for street trees.

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Section: Growth and Physiological Measurementsmentioning

confidence: 99%

Reduction in Water Stress for Tree Saplings Using Hydrogels in Soil

Kargar¹,

Suresh²,

Legrand³

et al. 2017

GEP

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“…The use of these reliable and advanced instruments for measurement of green pigment content is not restricted to controlled laboratory bioassays but can be extended to experiments in the plants' natural environment (Uddling et al, 2007;Ruiz-Espinoza et al, 2010;Neto et al, 2011;Cerovic et al, 2012). SPAD-502 chlorophyll meters have been used to obtain accurate measurements in arbitrary SPAD units reflecting absolute foliar Chl concentrations (Arunyanark et al, 2008;Marenco et al, 2009;Mielke et al, 2010;Ling et al, 2011;Shrestha et al, 2012). A significant linear relationship between SPAD readings and leaf nitrogen status in tissues of monocotyledonous species has been documented (Rostami et al, 2008;Swain and Sandip, 2010;Liu et al, 2012;Ghosh et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Chlorophyll Content of Aphid-Infested Seedling Leaves of Fifteen Maize Genotypes

Sytykiewicz¹,

Czerniewicz²,

Sprawka³

et al. 2013

Acta Biologica Cracoviensia Series Botanica

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We measured the total chlorophyll (Chl a+b) content in seedling leaves of fifteen maize cultivars infested by two studied aphid species (oligophagous Rhopalosiphum padi L., monophagous Sitobion avenae F.) 7 and 14 days after the beginning of infestation, using a SPAD-502 chlorophyll meter. Chlorophyll loss was more severe in R. padi-infested than in S. avenae-infested plants. Chlorophyll depletion was greater after long-term (14 days) than after short-term aphid infestation in the investigated host systems. Seedlings of Złota Karłowa and Tasty Sweet were more damaged by aphid feeding; Ambrozja and Płomyk plants were less damaged by aphid feeding.K Ke ey y w wo or rd ds s: : Total chlorophyll (Chl a+b) content, SPAD units, maize, Rhopalosiphum padi, Sitobion avenae.

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“…SPAD values of leaf samples were also measured during each harvest period using SPAD-502 meter. These values are proportional to the amount of chlorophyll present in the leaf chlorophyll content (Ling et al, 2011). The experimental data were subjected to analysis of variance according to Gomez and Gomez using M-Stat-C software.…”

Section: Methodsmentioning

confidence: 99%

“…However, its application requires effective treatment to safeguard public health and environment at economic cost (Anderson et al, 2001;Agunwamba, 2001;Sipala et al, 2003;Ling et al, 2011;Khan et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Response of Sorghum (Sorghum bicolor M.) to Three Irrigation Systems under Treated Waste water and Fresh Water Irrigations system

Alkhamisi¹,

Al-Jabri²,

Nadaf³

et al. 2017

JEBAS

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The treated wastewater is highly used as an alternate source of water for growing crops like sorghum for forage/fodder to livestock in Arabian Peninsula water deficit countries including Oman. In present study, high forage yielding sorghum variety, Superdan was investigated under three irrigation systems (Surface, Sub-surface and Drips) at Agriculture Research Station, Rumais, Oman, for its response to treated wastewater and fresh water irrigation conditions with respect to forage productivity. Study was conducted over continuous 11 forage harvests which started from summer season (April, 2013) and completed in mid-winter season (March, 2015). The results of study revealed that both mean green and dry matter yields/ cut and their total yield over 680 days were significantly higher under drip irrigation and it was followed by sub-surface and surface irrigation systems in both treated and freshwater irrigations. Water use efficiency (WUE) of sorghum was significantly higher under drip irrigation than under other irrigation systems not only with treated wastewater (2.54) but also with freshwater irrigation (2.49). The concentrations of heavy elements were either very low, low or in trace amount under the limits of toxicity threshold in both plant samples and soil samples. The present study demonstrated that forage sorghum can last till 11 cut-harvests spanning 680 days providing total green and dry matter yields of 508.30 t/ha and 143.88 t/ha respectively under treated wastewater irrigation compared to total green and dry matter yields of 498.26 t/ha and 144.58 t/ha, respectively under freshwater irrigation.

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Use of a SPAD-502 meter to measure leaf chlorophyll concentration in Arabidopsis thaliana

Cited by 353 publications

References 25 publications

Reduction in Water Stress for Tree Saplings Using Hydrogels in Soil

Reduction in Water Stress for Tree Saplings Using Hydrogels in Soil

Chlorophyll Content of Aphid-Infested Seedling Leaves of Fifteen Maize Genotypes

Response of Sorghum (Sorghum bicolor M.) to Three Irrigation Systems under Treated Waste water and Fresh Water Irrigations system

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