2010
DOI: 10.1007/s11120-010-9606-0
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Use of a SPAD-502 meter to measure leaf chlorophyll concentration in Arabidopsis thaliana

Abstract: The SPAD-502 meter is a hand-held device that is widely used for the rapid, accurate and non-destructive measurement of leaf chlorophyll concentrations. It has been employed extensively in both research and agricultural applications, with a range of different plant species. However, its utility has not been fully exploited in relation to the most intensively studied model organism for plant science research, Arabidopsis thaliana. Measurements with the SPAD-502 meter produce relative SPAD meter values that are … Show more

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Cited by 352 publications
(233 citation statements)
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“…The SPAD-502 meter measures leaf transmittance in the red (650 nm) and infrared (940 nm) bands. The SPAD meter value, typically between 0.0 and 50.0, is proportional to the amount of chlorophyll in the leaf [24]. Both NDVI and SPAD readings were measured and recorded every second week.…”
Section: Growth and Physiological Measurementsmentioning
confidence: 99%
“…The SPAD-502 meter measures leaf transmittance in the red (650 nm) and infrared (940 nm) bands. The SPAD meter value, typically between 0.0 and 50.0, is proportional to the amount of chlorophyll in the leaf [24]. Both NDVI and SPAD readings were measured and recorded every second week.…”
Section: Growth and Physiological Measurementsmentioning
confidence: 99%
“…The use of these reliable and advanced instruments for measurement of green pigment content is not restricted to controlled laboratory bioassays but can be extended to experiments in the plants' natural environment (Uddling et al, 2007;Ruiz-Espinoza et al, 2010;Neto et al, 2011;Cerovic et al, 2012). SPAD-502 chlorophyll meters have been used to obtain accurate measurements in arbitrary SPAD units reflecting absolute foliar Chl concentrations (Arunyanark et al, 2008;Marenco et al, 2009;Mielke et al, 2010;Ling et al, 2011;Shrestha et al, 2012). A significant linear relationship between SPAD readings and leaf nitrogen status in tissues of monocotyledonous species has been documented (Rostami et al, 2008;Swain and Sandip, 2010;Liu et al, 2012;Ghosh et al, 2013).…”
Section: Introductionmentioning
confidence: 99%
“…SPAD values of leaf samples were also measured during each harvest period using SPAD-502 meter. These values are proportional to the amount of chlorophyll present in the leaf chlorophyll content (Ling et al, 2011). The experimental data were subjected to analysis of variance according to Gomez and Gomez using M-Stat-C software.…”
Section: Methodsmentioning
confidence: 99%
“…However, its application requires effective treatment to safeguard public health and environment at economic cost (Anderson et al, 2001;Agunwamba, 2001;Sipala et al, 2003;Ling et al, 2011;Khan et al, 2012).…”
Section: Introductionmentioning
confidence: 99%