Use of a saliva‐based diagnostic test to identify tapeworm infection in horses in the UK

Lightbody, Kirsty L.; Matthews, Jacqueline B.; Kemp-Symonds, Jeremy; Lambert, Peter A.; Austin, Corrine J.

doi:10.1111/evj.12742

Cited by 22 publications

(28 citation statements)

References 34 publications

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“…In contrast to the coproscopically obtained prevalence, the high seroprevalence observed here is in line with the prevalences as determined in previous surveys at abattoirs in the region of Bavaria, Germany (28.5-38.0%) [6,9]. Recently, Lightbody et al [36] used the saliva-based ELISA in a longitudinal study with naturally infected horses from the UK and 15% initially tested positive and this value remained approximately constant in follow up visits after six and 12 months [36].…”

Section: Discussionsupporting

confidence: 92%

Investigations on the occurrence of tapeworm infections in German horse populations with comparison of different antibody detection methods based on saliva and serum samples

Jürgenschellert

Krücken

Austin³

et al. 2020

Parasites Vectors

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Background Effective and sustainable worm control in horses would benefit from detailed information about the current regional occurrence of tapeworms. Different diagnostic methods are currently available to detect Anoplocephala spp. infections in horses. However, the format as well as the sensitivity and specificity of the methods vary considerably. Methods A coprological, serological and questionnaire study was conducted to investigate the prevalence and risk factors of tapeworm infections on 48 horse farms in the region of Berlin and Brandenburg, Germany. In total, faecal samples of 484 horses were analysed using the double centrifugation/combined sedimentation-flotation and mini-FLOTAC. Serum (n = 481) and saliva (n = 365) samples were analysed by ELISAs to determine antibody levels against Anoplocephala spp. 12/13 kDa excretory/secretory (E/S) antigens. Results Cestode eggs were detected in 0.6% of faecal samples (farm prevalence 6.3%) without differences between the two methods. In contrast, antibodies against Anoplocephala spp. were detected in 16.2% (farm prevalence 52.1%) and in 29.5% (farm prevalence 75.7%) of the serum and saliva samples, respectively. Both ELISA based methods for detection of tapeworms reported a greater number of infected animals requiring treatment than were positively identified by coproscopy. Logistic regression analysis identified permanent pasture access, large pastures and regular pasture changes and high strongyle egg counts as risk factors for positive serum antibody responses to Anoplocephala spp. while last treatment with praziquantel was protective. Other protective factors were the presence of foals and high numbers of horses on the farm. Daily removal of faeces from the pasture and horse age did not have a significant effect. Conclusions The findings of the present serological investigation indicate that tapeworm prevalence in Berlin/Brandenburg horse farms is much higher than would be anticipated by using conventional/coproscopic analyses. Moreover, the majority of tapeworm-positive horses had not received a cestocidal drug at their last treatment. Considering the already known low sensitivity of the coproscopic detection, the equine veterinary diagnostics can be enhanced by the use of antibody detection methods such as the saliva-based ELISA.

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Section: Discussionsupporting

confidence: 92%

Investigations on the occurrence of tapeworm infections in German horse populations with comparison of different antibody detection methods based on saliva and serum samples

Jürgenschellert

Krücken

Austin³

et al. 2020

Parasites Vectors

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“…Monitoring after treatment revealed 4/6 colts and 3/6 fillies had reduced antibody levels whereas 2/6 colts and 3/6 fillies had increased/unchanged levels. This is in contrast to published research where salivary antibody levels reduce following treatment (Lightbody et al 2016(Lightbody et al , 2018. A fourth yearling (2020) presented as the first despite increased de-worming and decreased stocking density.…”

contrasting

confidence: 84%

Relationship Between Small Strongyles (Cyathostominae) and Horse Colic Occurrence

2021

Equine Veterinary Education

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Background: UK Gasterophilus prevalence in horses were last reported in 2005 (Morgan et al. 2005) with fewer G. Nasalis than previously reported by Coles and Pearson (2000). Climate change continues to alter annual weather patterns and diagnostic led worm control in managed horses has increased. Both factors may alter Gasterophilus spp. infection prevalence in UK horses. Objectives: Characterise the prevalence of G. Intestinalis and G. Nasalis in cadaver G/I tracts of managed and semi-feral horses in the UK. Methods: Stomachs and 30 cm of duodenum were taken from cadavers of managed (n = 50) and semi-feral (n = 50) horses slaughtered for human consumption in South-West England. Sample were collected during February and March 2019. Semi-feral animals comprised New Forrest and Welsh ponies aged 11.8 AE 8.9 years. Managed animals comprised Thoroughbreds and Irish Sport Horses aged 11.3 AE 6.8 years Parasite control practices were unknown. Instars were identified from cadavers, counted and weighed per animal. Data were analysed by generalised linear mixed effects models, Genstat version 19. Results: Prevalence of Gasterophilus spp. differed between managed and semi-feral horses for both species, weight of instars and number present (p > 0.001). For semi-feral horses 90% of the population, and 24% of the managed, had myiasis of one or both species. G. Intestinalis were more prevalent than G. Nasalis. with higher abundance in the semi-feral population. Conclusions: Gasterophilus spp. remain abundant in the South-West of the UK despite changes in weather and worm control practices. Parasite control techniques appear effective in managed horses, with greater threat of myiasis in semi-feral animals. Ethical animal research: Not required.

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“…For A perfoliata , positive ELISA diagnoses also contribute to the data. Although an ELISA indicating the presence of the species has been available for many years, a commercial saliva ELISA was developed more recently 3 and is likely to have influenced the number of submissions.…”

Section: Using the Quarterly Equine Disease Surveillance Database To mentioning

confidence: 99%

Using worm egg count data to detect and counter trends in equine helminth abundance

Dijk¹

2021

Veterinary Record

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Use of a saliva‐based diagnostic test to identify tapeworm infection in horses in the UK

Abstract: Compared with an all-group treatment strategy, the diagnostic-led approach used here considerably reduced application of anticestode anthelmintics. This could reduce selection pressure for anthelmintic resistance.

Cited by 22 publications

References 34 publications

Investigations on the occurrence of tapeworm infections in German horse populations with comparison of different antibody detection methods based on saliva and serum samples

Investigations on the occurrence of tapeworm infections in German horse populations with comparison of different antibody detection methods based on saliva and serum samples

Relationship Between Small Strongyles (Cyathostominae) and Horse Colic Occurrence

Using worm egg count data to detect and counter trends in equine helminth abundance

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