Use of a radioimmunoassay technique to measure the titer of anti‐cow‐zona antibodies in marmoset monkeys

Hulme, M. J.; Aitken, R. John; Richardson, David W.

doi:10.1002/mrd.1120050307

Cited by 6 publications

(1 citation statement)

References 23 publications

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“…A single experimental profile is shown from one of a group of marmosets immu¬ nized against pig zonae pellucidae, using alum as adjuvant. Antibody titre was monitored by radioimmunoassay (Hulme et al, 1982). Details of the management of the marmosets and the methods used to monitor their ovarian cyclicity are given by Hearn et al (1975).…”

Section: In-vivo Studiesmentioning

confidence: 99%

Contraceptive potential of antibodies to the zona pellucida

Henderson¹,

Hulme²,

Aitken³

1988

Reproduction

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The notion of a contraceptive vaccine based on gamete-specific surface antigens was first proposed over a decade ago, as the result of in-vitro and in-vivo studies, and in recent years has been the subject of intensive research. In particular, the zona pellucida has attracted much attention as a potential target for immunological intervention in the fertilization process. Such is the rapidly-expanding nature of research into the biochemical and biological characterization of this structure, that a review of the implications for the development of a contraceptive vaccine seems timely.

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Section: In-vivo Studiesmentioning

confidence: 99%

Contraceptive potential of antibodies to the zona pellucida

Henderson¹,

Hulme²,

Aitken³

1988

Reproduction

Self Cite

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Studies on the stability of ¹²⁵I‐purified porcine zona antigen (PPZA) obtained by chloramine‐T glucose oxidase with lactoperoxidase and N‐bromosuccinimide lodination procedures

1985

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A purified pig zona antigen (PPZA) was labeled with 125I by chloramine‐T (CL‐T), glucose oxidase with lactoperoxidase (GO‐LP), and N‐bromosuccinimide (N‐BS) iodination procedures. The 125I‐PPZA fractions thus obtained were reconstituted to a concentration of 25,000 dpm/0.1 ml and tested at weekly intervals for binding capacity to a fixed dilution (1:400,000) of PPZA antibody. The 125I‐PPZA obtained by the N‐BS procedure showed greater stability compared to that obtained by CL‐T and GO‐LP methods. The nonspecific binding levels for the N‐BS and GO‐LP methods were comparable and significantly lower than for the CL‐T method.

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Analysis of the biological properties of antibodies raised against intact and deglycosylated porcine zonae pellucidae

1987

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Zonae pellucidae (ZP) were isolated from 1,500 porcine ovaries and heat solubilized to generate approximately 15 mg ZP glycoprotein. Analysis of this material by isoelectric focusing, one-dimensional electrophoresis, and gas chromatography indicated the presence of a major glycoprotein species that exhibited considerable microheterogeneity with respect to its charge (pI 7.5-3.5) and molecular mass (45-85 kDa) and that contained 39.6% carbohydrate, predominantly N-acetylglucosamine. Chemical deglycosylation of porcine ZP using trifluoromethanesulphonic acid (TFMS) resulted in the production of five discrete protein bands on one-dimensional sodium dodecyl sulphate/polyacrylamide gel electrophoresis (SDS/PAGE) with molecular masses of 66, 52, 36, 32, and 16 kDa. Antisera raised in rabbits and marmosets to ZP and/or deglycosylated ZP (DGZP) were used in immunoblotting experiments to demonstrate the retention of immunogenicity by DGZP and the cross-reactivity of the antisera with their heterologous antigen. These studies indicated that antisera that were capable of inhibiting the fertility of primates in vivo and the penetration of the human ZP in vitro reacted preferentially with 3 of the 5 products of deglycosylation, with molecular masses of 66, 52, and 36 kDa. Anti-DGZP antibodies were also shown to interact with intact porcine and human ZP and, with the latter, to block the ability of human spermatozoa to both bind to and penetrate this structure.

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Use of a radioimmunoassay technique to measure the titer of anti‐cow‐zona antibodies in marmoset monkeys

Cited by 6 publications

References 23 publications

Contraceptive potential of antibodies to the zona pellucida

Contraceptive potential of antibodies to the zona pellucida

Studies on the stability of ¹²⁵I‐purified porcine zona antigen (PPZA) obtained by chloramine‐T glucose oxidase with lactoperoxidase and N‐bromosuccinimide lodination procedures

Analysis of the biological properties of antibodies raised against intact and deglycosylated porcine zonae pellucidae

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Use of a radioimmunoassay technique to measure the titer of anti‐cow‐zona antibodies in marmoset monkeys

Cited by 6 publications

References 23 publications

Contraceptive potential of antibodies to the zona pellucida

Contraceptive potential of antibodies to the zona pellucida

Studies on the stability of 125I‐purified porcine zona antigen (PPZA) obtained by chloramine‐T glucose oxidase with lactoperoxidase and N‐bromosuccinimide lodination procedures

Analysis of the biological properties of antibodies raised against intact and deglycosylated porcine zonae pellucidae

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Studies on the stability of ¹²⁵I‐purified porcine zona antigen (PPZA) obtained by chloramine‐T glucose oxidase with lactoperoxidase and N‐bromosuccinimide lodination procedures