Use of a purified outer membrane protein F (porin) preparation of Pseudomonas aeruginosa as a protective vaccine in mice

Gilleland, H. E.; Parker, Malcolm G.; Matthews, J. M.; Berg, Rodney D.

doi:10.1128/iai.44.1.49-54.1984

Cited by 124 publications

(63 citation statements)

References 29 publications

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“…Our laboratory has been examining the use of protein F (OprF) from the outer membrane of P. aeruginosa as a protective vaccine. We have used a number of rodent models of infection [24,27,31,39,40] with P. aeruginosa to demonstrate the protective e⁄cacy of protein F in various forms. These include the use of the puri¢ed protein [27,31,39,41], synthetic peptides coupled to a carrier protein [26,40,42], a chimeric virus engineered to contain one or two epitopes of protein F within a viral surface protein Table 5 Protection a¡orded upon passive administration of the various antisera…”

Section: Discussionmentioning

confidence: 99%

Enhancement of the protective efficacy of an oprF DNA vaccine against Pseudomonas aeruginosa

Price

2002

FEMS Immunology and Medical Microbiology

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The outer membrane protein F gene (oprF) of Pseudomonas aeruginosa was recently shown by us to protect mice from P. aeruginosa chronic pulmonary infection when used as a DNA vaccine administered by three biolistic (gene gun) intradermal inoculations given at 2-week intervals. In the present study, we used two different strategies to improve the protective efficacy of the DNA vaccine. In the first strategy, mice were primed with two biolistic intradermal inoculations with the oprF vaccine and then were given a final intramuscular booster immunization containing either a synthetic peptide-keyhole limpet hemocyanin (KLH) conjugate or a chimeric influenza virus. Both the synthetic peptide conjugate and the chimeric virus contained peptide 10, a previously identified immunoprotective epitope of protein F. The second strategy involved the addition of a second outer membrane protein to the vaccine. DNA encoding a fusion protein comprised of the C-terminal half of protein F fused to OprI was administered by three biolistic intradermal inoculations. Challenge with P. aeruginosa in a chronic pulmonary infection model demonstrated that boosting with the chimeric virus (but not with peptide-KLH) or adding oprI to the DNA vaccine significantly enhanced protection as compared to that afforded by the oprF vaccine given alone. Thus, both strategies appear to augment the protection afforded by an oprF-only DNA vaccine.

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Section: Discussionmentioning

confidence: 99%

Enhancement of the protective efficacy of an oprF DNA vaccine against Pseudomonas aeruginosa

Price

2002

FEMS Immunology and Medical Microbiology

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“…The molecular weights of proteins F and H were calculated to be 33 052 and 17 532 Da, respectively, being in the range of those reported for the same proteins in Ps. aeruginosa (Yoshimura et al 1983;Gilleland et al 1984Gilleland et al , 1988.…”

Section: Dc-7 (F) and Nt-19 (G) Protein Markers (A) As Inmentioning

confidence: 99%

A sandwich enzyme‐linked immunosorbent assay (ELISA) for detection of Pseudomcnas fluorescens and related psychrotrophic bacteria in refrigerated milk

González

Martı́n

Garcı́a

et al. 1993

Journal of Applied Bacteriology

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A sandwich ELISA (enzyme-linked immunosorbent assay) was developed for detection of Pseudomonas fluorescens and related psychrotrophic bacteria in refrigerated milk. Polyclonal antibodies were raised in rabbits against protein F from the cell envelope of Pseudomonas fluorescens AH-70. The anti-protein F antibodies (anti-PF) bound to the wells of a microtitre plate were used to capture this protein from the micro-organisms on milk samples. Further immunorecognition of the captured antigen was attained with the same anti-PF antibodies conjugated to biotin. ExtrAvidin-peroxidase was used to detect the biotinylated antibodies bound to their specific antigens. Subsequent enzymic conversion of substrate gave clear absorbance differences when assaying milk samples containing Ps. fluorescens strains of different origin as well as related psychrotropic micro-organisms.

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“…Early on we surveyed the protective abilities of a variety of puri¢ed OM proteins (D2, E, F, G, and H), reaching the conclusion that protein F had the greatest vaccine potential [2]. Protein F puri¢ed from the OM of P. aeruginosa was shown to a¡ord sig-ni¢cant protection in rodent models of systemic infection [3], burned mice [4], and chronic pulmonary infection [5]. Recombinant protein F puri¢ed from Escherichia coli was shown to retain vaccine e¤cacy in the burned mouse [2] and the rat chronic pulmonary infection [6] models.…”

Section: Introductionmentioning

confidence: 99%

Chimeric animal and plant viruses expressing epitopes of outer membrane protein F as a combined vaccine against Pseudomonas aeruginosa lung infection

Gilleland

2000

FEMS Immunology and Medical Microbiology

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Outer membrane protein F of Pseudomonas aeruginosa has vaccine efficacy against infection by P. aeruginosa as demonstrated in a variety of animal models. Through the use of synthetic peptides, three surface-exposed epitopes have been identified. These are called peptides 9 (aa 261-274 in the mature F protein, TDAYNQKLSERRAN), 10 (aa 305-318, NATAEGRAINRRVE), and 18 (aa 282-295, NEYGVEGGRVNAVG). Both the peptide 9 and 10 epitopes are protective when administered as a vaccine. In order to develop a vaccine that is suitable for use in humans, including infants with cystic fibrosis, the use of viral vector systems to present the protective epitopes has been investigated. An 11-amino acid portion of epitope 10 (AEGRAINRRVE) was successfully inserted into the antigenic B site of the hemagglutinin on the surface of influenza virus. This chimeric influenza virus protects against challenge with P. aeruginosa in the mouse model of chronic pulmonary infection. Attempts to derive a chimeric influenza virus carrying epitope 9 have been unsuccessful. A chimeric plant virus, cowpea mosaic virus (CPMV), with epitopes 18 and 10 expressed in tandem on the large coat protein subunit (CPMV-PAE5) was found to elicit antibodies that reacted exclusively with the 10 epitope and not with epitope 18. Use of this chimeric virus as a vaccine afforded protection against challenge with P. aeruginosa in the mouse model of chronic pulmonary infection. Chimeric CPMVs with a single peptide containing epitopes 9 and 18 expressed on either of the coat proteins are in the process of being evaluated. Epitope 9 was successfully expressed on the coat protein of tobacco mosaic virus (TMV), and this chimeric virus is protective when used as a vaccine in the mouse model of chronic pulmonary infection. However, initial attempts to express epitope 10 on the coat protein of TMV have been unsuccessful. Efforts are continuing to construct chimeric viruses that express both the 9 and 10 epitopes in the same virus vector system. Ideally, the use of a vaccine containing two epitopes of protein F is desirable in order to greatly reduce the likelihood of selecting a variant of P. aeruginosa that escapes protective antibodies in immunized humans via a mutation in a single epitope within protein F. When the chimeric influenza virus containing epitope 10 and the chimeric TMV containing epitope 9 were given together as a combined vaccine, the immunized mice produced antibodies directed toward both epitopes 9 and 10. The combined vaccine afforded protection against challenge with P. aeruginosa in the chronic pulmonary infection model at approximately the same level of efficacy as provided by the individual chimeric virus vaccines. These results prove in principle that a combined chimeric viral vaccine presenting both epitopes 9 and 10 of protein F has vaccine potential warranting continued development into a vaccine for use in humans.

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Use of a purified outer membrane protein F (porin) preparation of Pseudomonas aeruginosa as a protective vaccine in mice

Cited by 124 publications

References 29 publications

Enhancement of the protective efficacy of an oprF DNA vaccine against Pseudomonas aeruginosa

Enhancement of the protective efficacy of an oprF DNA vaccine against Pseudomonas aeruginosa

A sandwich enzyme‐linked immunosorbent assay (ELISA) for detection of Pseudomcnas fluorescens and related psychrotrophic bacteria in refrigerated milk

Chimeric animal and plant viruses expressing epitopes of outer membrane protein F as a combined vaccine against Pseudomonas aeruginosa lung infection

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