Use of a prediction method for early pregnancy status utilizing receiver operating characteristic curve analysis of peripheral blood leukocyte interferon-stimulated genes in Japanese-Black cattle

Yoshino, Hitomi; Kizaki, Keiichiro; Iga, Kosuke; Hirata, Toh-Ichi; Matsuda, Hideo; Yamanouchi, T.; Hashiyada, Y.; Toji, Noriyuki; Ishiguro‐Oonuma, Toshina; Takahashi, Toshio; Hashizume, Kazuyoshi

doi:10.1016/j.anireprosci.2020.106283

Cited by 8 publications

(13 citation statements)

References 25 publications

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“…Similarly, Yoshino et al. (2023) observed that the expression of ISGs was significantly higher in pregnant cattle than in non‐pregnant and late embryonic death cattle on day 21 of gestation. Additionally, in buffalo cows, ISG15 proved to have the best diagnostic performance in differentiating between pregnant and embryonic mortality cows (AUC = .980, p < .01) at day 19 after AI, while the sensitivity and specificity of the optimal threshold were 88.2% and 100%, respectively (Casano et al., 2023).…”

Section: Ifnt and Isgsmentioning

confidence: 80%

Recent advances in early pregnancy loss diagnosis in dairy cows: New approaches

Gui,

Dai,

Guo

et al. 2024

Reprod Domestic Animals

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Early pregnancy loss is a primary cause of low reproductive rates in dairy cows, posing severe economic losses to dairy farming. The accurate diagnosis of dairy cows with early pregnancy loss allows for oestrus synchronization, shortening day open, and increasing the overall conception rate of the herd. Several techniques are available for detecting early pregnancy loss in dairy cows, including rectal ultrasound, circulating blood progesterone, and pregnancy‐associated glycoproteins (PAGs). Yet, there is a need to improve on existing techniques and develop novel strategies to identify cows with early pregnancy loss accurately. This manuscript reviews the applications of rectal ultrasound, circulating blood progesterone concentration, and PAGs in the diagnosis of pregnancy loss in dairy cows. The manuscript also discusses the recent progress of new technologies, including colour Doppler ultrasound (CDUS), interferon tau‐induced genes (ISGs), and exosomal miRNA in diagnosing pregnancy loss in dairy cows. This study will provide an option for producers to re‐breed cows with pregnancy loss, thereby reducing the calving interval and economic costs. Meanwhile, this manuscript might also act as a reference for exploring more economical and precise diagnostic technologies for early pregnancy loss in dairy cows.

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Section: Ifnt and Isgsmentioning

confidence: 80%

Recent advances in early pregnancy loss diagnosis in dairy cows: New approaches

Gui,

Dai,

Guo

et al. 2024

Reprod Domestic Animals

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“…They then showed that measurement of ISG15 mRNA could provide a reliable indicator of early pregnancy on day 18 of gestation in heifers but not in mature cows (81). These findings have since been confirmed in beef breeds using both polymorphonuclear neutrophils and peripheral blood mononuclear cells (82,83) and extending to a number of other ISGs (84). Although the results of ISG15 up-regulation in early pregnancy were highly repeatable between studies, de Melo et al (85) found that ISG15 measurement was less reliable for confirmation of pregnancy than the use of circulating pregnancy-associated glycoprotein or Doppler ultrasound.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Ability of High and Low Virulence Strains of Non-cytopathic Bovine Viral Diarrhea Virus-1 to Modulate Expression of Interferon Tau Stimulated Genes in Bovine Endometrium

et al. 2021

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Bovine Viral Diarrhea virus (BVDV) is a pestivirus with a single-stranded, positive sense RNA genome. It is endemic in many cattle populations, causing major economic losses in part due to reduced fertility. BVDV exhibits great genetic diversity and is classified as type 1 or 2 (BVDV-1, BVDV-2) with either non-cytopathogenic (ncp) or cytopathogenic (cp) biotypes. Differing strains of ncpBVDV differ in virulence, affecting clinical outcome. BVDV replicates in the reproductive tract, affecting host immunity and embryo survival. This study used an in vitro model of primary bovine endometrial cell cultures to compare the effects of two BVDV ncp type 1a strains of differing virulence (termed HO and KY) on endometrial transcription of candidate interferon stimulated genes (ISG) using qPCR. Half the cultures were stimulated with interferon tau (IFNT, the conceptus produced pregnancy recognition factor) in the presence or absence of viral infection. Cultures were replicated on cells from 10 BVDV-free cows. IFNT treatment stimulated transcription of 10 candidate ISGs, whereas both ncpBVDV-1 strains alone inhibited transcription of 8/10 ISGs. In combined BVDV-1+IFNT cultures, the stimulatory effect of IFNT on expression of GBP4, ISG15, HERC5, RSAD2, IFIH1, IFIT3, and MX1 was significantly inhibited by HO, but only ISG15, RSAD2, IFI27, and IFIT3 were decreased by KY. Inhibition by HO was generally greater. The IFNT-induced expression of TRIM56 was, however, increased by HO. These data show that HO, the more virulent ncpBVDV-1 strain, has a greater capacity to inhibit key antiviral pathways. These differences need confirmation at the protein level but may influence immune tolerance of the host. They could also reduce fertility by increasing uterine susceptibility to bacterial infection and disrupting IFNT-mediated pregnancy recognition.

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“…Blood collection, RNA extraction, and RT-qPCR were performed as previously described [ 26 ]. Approximately 3 mL of peripheral blood was collected in PAXgene blood RNA tubes (Qiagen, Hilden, Germany) and incubated at room temperature for 2 h. RNA was extracted using an RNA extraction kit (PAXgene Blood RNA Kit; Qiagen).…”

Section: Methodsmentioning

confidence: 99%

“…Recently, ISG expression in peripheral blood leukocytes (PBLs) has been examined as a biomarker for predicting the gestational status during maternal recognition in ruminants [ 20 , 21 , 22 ]. The prediction efficiency of ISGs, specifically ISG15 and MX2 , for the establishment of gestation has been confirmed as reliable biomarkers in cattle [ 21 , 23 , 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Interferon-Stimulated Gene Expression in Peripheral Blood Leucocytes as a Convenient Prediction Marker for Embryo Status in Embryo-Transferred Japanese Black Cows during the Peri-Implantation Period

Yoshino

Kizaki

Hirata

et al. 2023

Veterinary Sciences

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Pregnancy diagnosis during early gestation is important for cattle reproduction. The expression of interferon-stimulated genes (ISGs) in peripheral blood leukocytes (PBLs) was studied in embryo-transferred (ET) Japanese Black cattle. ISGs in PBLs—ISG15, MX1, MX2, and OAS1—were detected in multiple ovulation ET cattle using a real-time quantitative polymerase chain reaction, and receiver operating characteristic (ROC) curve analysis was performed. Gestational status was predicted using the average ISG levels during the normal estrous cycle (AVE) and the Youden index from the ROC curve analysis as cutoff values. The ISG15, MX1, and MX2 levels were significantly higher in pregnant cattle (n = 10) than in non-pregnant cattle (n = 23) on gestation day 21, whereas the levels of all ISGs were similar between non-pregnant and non-pregnant cattle with late embryonic death (n = 7). ISG15, MX1, and MX2 appropriately predicted the gestational status of ET cows. The statistical evaluation of the diagnostic accuracy in ET cows on day 21 of gestation presented higher values of sensitivity, specificity, accuracy, and positive predictive values of ISG15, MX1, and MX2 using the Youden index than using the AVE. Therefore, ISG15, MX1, and MX2 are excellent biomarkers of gestational status during the peri-implantation period in ET cattle.

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Use of a prediction method for early pregnancy status utilizing receiver operating characteristic curve analysis of peripheral blood leukocyte interferon-stimulated genes in Japanese-Black cattle

Cited by 8 publications

References 25 publications

Recent advances in early pregnancy loss diagnosis in dairy cows: New approaches

Recent advances in early pregnancy loss diagnosis in dairy cows: New approaches

Comparison of the Ability of High and Low Virulence Strains of Non-cytopathic Bovine Viral Diarrhea Virus-1 to Modulate Expression of Interferon Tau Stimulated Genes in Bovine Endometrium

Interferon-Stimulated Gene Expression in Peripheral Blood Leucocytes as a Convenient Prediction Marker for Embryo Status in Embryo-Transferred Japanese Black Cows during the Peri-Implantation Period

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