Use of a novel induced spawning technique for the first reported captive spawning of Tetraodon nigroviridis

Watson, Craig A.; Hill, Jeffrey E.; Graves, Jeffrey S.; Wood, Amy Louise; Kilgore, Kathy Heym

doi:10.1016/j.margen.2009.04.004

Cited by 23 publications

(18 citation statements)

References 15 publications

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“…Embryos for recording of the stage series were produced using the induced spawning technique developed by Watson et al 12 In brief, female gonads were treated with Chorulon Ò (human chorionic gonadotropin; Merck Pharmaceuticals, Inc.) to stimulate ovulation. Chorulon was directly injected into the ovary of anaesthetized females through a catheter inserted into the oviduct.…”

Section: Methodsmentioning

confidence: 99%

“…12 Batches of approximately 200 embryos were kept in Ø 9 cm Petri dishes filled with artificial seawater (33 ppt salinity). The seawater was replaced twice a day.…”

Section: Embryo Culturementioning

confidence: 99%

“…11 Watson et al demonstrated a simple and reproducible breeding protocol for tetraodon, which involves in vitro egg fertilization after ovarian lavage. 12 Tetraodon embryos are transparent similar to zebrafish or medaka embryos, their development takes around 80 h to hatching, and over 2000 eggs are produced by the female. The generation time of tetraodon is around 9 months (Watson et al, unpublished data).…”

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confidence: 99%

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Description of Embryonic Development of Spotted Green Pufferfish (Tetraodon nigroviridis)

Zaucker

Bodur²,

Crollius³

et al. 2014

Zebrafish

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Pufferfish species of the Tetraodontidae family carry the smallest genomes among vertebrates. Their compressed genomes are thought to be enriched for functional DNA compared to larger vertebrate genomes, and they are important models for comparative genomics. The significance of pufferfish as model organisms in comparative genomics is due to the availability of two sequenced genomes, that of spotted green pufferfish (Tetraodon nigroviridis) and fugu (Takifugu rubripes). However, there is only a very limited utilization of pufferfish as an experimental model organism, due to the lack of established husbandry and developmental genetics protocols. In this study, we provide the first description of the normal embryonic development of Tetraodon nigroviridis. Embryos were obtained by in vitro fertilization of eggs, and subsequent development was monitored by brightfield microscopy at constant temperature. Tetraodon development was divided into distinct stages based on diagnostic morphological features, which were adopted from published literature on normal development of other fish species like medaka (Oryzias latipes), zebrafish (Danio rerio), and fugu. Tetraodon embryos show more similar morphologies to medaka than to zebrafish, reflecting its phylogenetic position. The early developmental stage series described in this study forms the foundation for the utilization of tetraodon as an experimental model organism for comparative developmental studies.

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Section: Methodsmentioning

confidence: 99%

“…12 Batches of approximately 200 embryos were kept in Ø 9 cm Petri dishes filled with artificial seawater (33 ppt salinity). The seawater was replaced twice a day.…”

Section: Embryo Culturementioning

confidence: 99%

mentioning

confidence: 99%

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Description of Embryonic Development of Spotted Green Pufferfish (Tetraodon nigroviridis)

Zaucker

Bodur²,

Crollius³

et al. 2014

Zebrafish

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“…We sought evolutionarily conserved features of promoters by comparing zebrafish data to that of the small genome species Tetraodon nigroviridis (spotted green pufferfish) ( Jaillon et al 2004). We bred Tetraodon in the laboratory (Watson et al 2009; A Zaucker, T Bodur, J Gehrig, Y Hadzhiev, F Loosli, H Roest Crollius, C Watson, F Müller, in prep. ) and carried out CAGE analysis of its promoterome.…”

Section: Evolutionary Conservation Of Sequence Characteristics Suggesmentioning

confidence: 99%

Dynamic regulation of the transcription initiation landscape at single nucleotide resolution during vertebrate embryogenesis

et al. 2013

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Spatiotemporal control of gene expression is central to animal development. Core promoters represent a previously unanticipated regulatory level by interacting with cis-regulatory elements and transcription initiation in different physiological and developmental contexts. Here, we provide a first and comprehensive description of the core promoter repertoire and its dynamic use during the development of a vertebrate embryo. By using cap analysis of gene expression (CAGE), we mapped transcription initiation events at single nucleotide resolution across 12 stages of zebrafish development. These CAGE-based transcriptome maps reveal genome-wide rules of core promoter usage, structure, and dynamics, key to understanding the control of gene regulation during vertebrate ontogeny. They revealed the existence of multiple classes of pervasive intra- and intergenic post-transcriptionally processed RNA products and their developmental dynamics. Among these RNAs, we report splice donor site-associated intronic RNA (sRNA) to be specific to genes of the splicing machinery. For the identification of conserved features, we compared the zebrafish data sets to the first CAGE promoter map of Tetraodon and the existing human CAGE data. We show that a number of features, such as promoter type, newly discovered promoter properties such as a specialized purine-rich initiator motif, as well as sRNAs and the genes in which they are detected, are conserved in mammalian and Tetraodon CAGE-defined promoter maps. The zebrafish developmental promoterome represents a powerful resource for studying developmental gene regulation and revealing promoter features shared across vertebrates.

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“…The Supergestran contains mammalian GnRH ([D-Ala6]GnRHProNhet) analogue. The Chorulon has been used for artificial reproduction of African catfish (Clarias gariepinus) (Richter et al 1987;Mollah and Tan 1983), rainbow sharks (Labeo erythrurus) (Shireman and Gildea 1989), redtail black sharks (Labeo bicolor) (Shireman and Gildea 1989), and of pufferfish (Tetraodon nigroviridis) (Watson et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Hormonal induction of ovulation in pikeperch (Sander lucioperca L.) using human chorionic gonadotropin (hCG) and mammalian GnRH analogue

et al. 2012

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The aim of the present study was to study spawning stimulation in artificial reproduction of females pikeperch (Sander lucioperca L.) using ''Chorulon'' containing the human chorionic gonadotropin (hCG) and compare with ''Supergestran'' containing a mammalian GnRH ([D-Ala 6 ]GnRHProNhet) analogue. The females were divided into eleven experimental groups and injected with hCG at 250, 500, 750, and 1 000 IU kg -1 body weight (BW) and mGnRHa at 1, 2.5, 5, 10, 25, and 50 lg kg -1 BW. In all treatments, a single intramuscular injection of hormone was performed. Control group was injected with 0.9 % NaCl, 0.9 cm 3 kg -1 BW. The average percentages of ovulating females were 88.5 ± 12.3 and 80.8 ± 10.9 % in hCG-and mGnRHa-treated groups, respectively. The average diameter of eggs was 0.95 ± 0.06 and 0.98 ± 0.06 mm in hCG-and mGnRHatreated groups, respectively. Neither ovulation rate nor diameter of egg was statistically differed among hormonally treated groups. Statistical difference was observed only in hatching rate, where the average were 73.6 ± 14.4 and 50.6 ± 17.7 % in hCG and mGnRHa-treated groups, respectively. Among hormonally treated groups, the best results were observed in groups treated with hCG at 500 and 750 IU kg -1 and in groups treated with mGnRHa at 25 lg kg -1 . No ovulation was observed in the control group. This study indicated successful ovulation in pikeperch using a single intramuscular injection of hCG or mGnRHa analogue.

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Use of a novel induced spawning technique for the first reported captive spawning of Tetraodon nigroviridis

Cited by 23 publications

References 15 publications

Description of Embryonic Development of Spotted Green Pufferfish (Tetraodon nigroviridis)

Description of Embryonic Development of Spotted Green Pufferfish (Tetraodon nigroviridis)

Dynamic regulation of the transcription initiation landscape at single nucleotide resolution during vertebrate embryogenesis

Hormonal induction of ovulation in pikeperch (Sander lucioperca L.) using human chorionic gonadotropin (hCG) and mammalian GnRH analogue

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