Use of a novel erbium laser in a Yucatan minipig: A study of residual thermal damage, ablation, and wound healing as a function of pulse duration†

Ross, E. Victor; McKinlay, Joseph R.; Sajben, Francis P.; Miller, Charles H.; Barnette, David J.; Meehan, Kenneth J.; Chhieng, Norak P.; Deavers, Mickey J.; Zelickson, Brian D.

doi:10.1002/lsm.10030

Cited by 37 publications

(27 citation statements)

References 24 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, various tissue alterations, primarily photochemical, thermal and non-linear processes, are known to occur [19-22], which may change the optical properties of tissue. Investigating the specific tissue alterations, the subsequent changes of optical properties and its impact on optical tissue differentiation present a crucial step towards implementing a remote optical feedback mechanism for laser ablation in a clinical setting.…”

Section: Introductionmentioning

confidence: 99%

The impact of laser ablation on optical soft tissue differentiation for tissue specific laser surgery-an experimental ex vivo study

et al. 2012

View full text Add to dashboard Cite

BackgroundOptical diffuse reflectance can remotely differentiate various bio tissues. To implement this technique in an optical feedback system to guide laser surgery in a tissue-specific way, the alteration of optical tissue properties by laser ablation has to be taken into account. It was the aim of this study to evaluate the general feasibility of optical soft tissue differentiation by diffuse reflectance spectroscopy under the influence of laser ablation, comparing the tissue differentiation results before and after laser intervention.MethodsA total of 70 ex vivo tissue samples (5 tissue types) were taken from 14 bisected pig heads. Diffuse reflectance spectra were recorded before and after Er:YAG-laser ablation. The spectra were analyzed and differentiated using principal component analysis (PCA), followed by linear discriminant analysis (LDA). To assess the potential of tissue differentiation, area under the curve (AUC), sensitivity and specificity was computed for each pair of tissue types before and after laser ablation, and compared to each other.ResultsOptical tissue differentiation showed good results before laser exposure (total classification error 13.51%). However, the tissue pair nerve and fat yielded lower AUC results of only 0.75. After laser ablation slightly reduced differentiation results were found with a total classification error of 16.83%. The tissue pair nerve and fat showed enhanced differentiation (AUC: 0.85). Laser ablation reduced the sensitivity in 50% and specificity in 80% of the cases of tissue pair comparison. The sensitivity of nerve–fat differentiation was enhanced by 35%.ConclusionsThe observed results show the general feasibility of tissue differentiation by diffuse reflectance spectroscopy even under conditions of tissue alteration by laser ablation. The contrast enhancement for the differentiation between nerve and fat tissue after ablation is assumed to be due to laser removal of the surrounding lipid-rich nerve sheath. The results create the basis for a guidance system to control laser ablation in a tissue-specific way.

show abstract

Section: Introductionmentioning

confidence: 99%

The impact of laser ablation on optical soft tissue differentiation for tissue specific laser surgery-an experimental ex vivo study

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Epidermal loss also increases the risk of infection, scarring, and pigmentary changes. Stimulation of new extracellular matrix deposition appears to be the primary mechanism for wrinkle reduction [4][5][6]. Since the microscopic changes associated with wrinkles occur primarily in the dermis, epidermal damage may be unnecessary for treatment of facial rhytides [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Intradermally focused infrared laser pulses: Thermal effects at defined tissue depths

et al. 2005

View full text Add to dashboard Cite

show abstract

“…As in many areas of medicine, the post-laser result remains to rely on histopathologic examination in the evaluating the epidermal, dermal, vascular and appendage components of skin. 13,14 The drawbacks in this procedure are invasive in nature and that the fixation and processing procedures render the studying of real time light-tissue interaction impossible.…”

Section: Introductionmentioning

confidence: 99%

Monitoring laser-tissue interaction by non-linear optics

et al. 2008

View full text Add to dashboard Cite

To assess laser therapies in clinical practice, histologic examinations were commonly used. But histologic examinations were invasive and not real-time in nature. In this work, we validate multiphoton microscopy as a useful modality in evaluating laser-tissue reaction in vivo. Multiphoton microscopy based on femtosecond titanium-sapphire laser system were used to evaluate autoflurescence (AF) and second harmonic generation (SHG). Nude mouse skin was irradiated with Erbium:YAG laser at low to high fluence. High resolutional images can be obtained by multiphoton microscopy. At low fluence, Erbium:YAG laser can selectively loosen compact stratum corneum with minimal injury to basal layer. At high fluence, ablated keratinocytes and residual debris can be imaged. The laser thermal effect on dermis could be measured by SHG signals of collagen fibers. SHG decreased as laser fluence increased. Multiphoton microscopy is a useful in-vivo technique in evaluating ablative and thermal effects of Erbium:YAG laser on nude mouse skin.

show abstract

Use of a novel erbium laser in a Yucatan minipig: A study of residual thermal damage, ablation, and wound healing as a function of pulse duration†

Abstract: An erbium laser with a variable macropulse pulsewidth was capable of achieving RTD of up to 80 mum. Even greater RTD depths may be obtainable with future manipulations of fluence and pulse duration.

Cited by 37 publications

References 24 publications

The impact of laser ablation on optical soft tissue differentiation for tissue specific laser surgery-an experimental ex vivo study

The impact of laser ablation on optical soft tissue differentiation for tissue specific laser surgery-an experimental ex vivo study

Intradermally focused infrared laser pulses: Thermal effects at defined tissue depths

Monitoring laser-tissue interaction by non-linear optics

Contact Info

Product

Resources

About