Use of a newly-developed portable capillary electrophoresis analyser to detect drugs of abuse in oral fluid: A case study

Saar-Reismaa, Piret; Brilla, Chelsa-Ann; Leiman, Kristiina; Kaljurand, Mihkel; Vaher, Merike; Kulp, Maria; Mazina-Šinkar, Jekaterina

doi:10.1016/j.talanta.2019.120662

Cited by 17 publications

(9 citation statements)

References 23 publications

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“…After method optimization, the authors proceeded with the validation and the analysis of samples. The results were confirmed determining the same analytes with the HPLC-MS method [32].…”

Section: Capillary Electromigration Techniquessupporting

confidence: 58%

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Potentiality of miniaturized techniques for the analysis of drugs of abuse

et al. 2021

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Capillary electromigration (CE) and liquid chromatographic techniques (CLC/nano‐LC) are miniaturized techniques offering distinct advantages over conventional ones in the field of separation science. Among these, high efficiency, high chromatographic resolution, and use of minute volumes of both mobile phase and sample volumes are the most important. CE and CLC/nano‐LC have been applied to the analysis of many compounds including peptides, proteins, drugs, enantiomers, ions, etc. Over the years, the methods described here have also been used for the analysis of compounds of clinical, forensic, and toxicological interest. In this review article, the main features of the mentioned techniques are summarized. Their potentiality for the analysis of drugs of abuse are discussed. Some selected applications in this field in the period of 2015–present are also reported.

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“…After method optimization, the authors proceeded with the validation and the analysis of samples. The results were confirmed determining the same analytes with the HPLC-MS method [32].…”

Section: Capillary Electromigration Techniquessupporting

confidence: 58%

“…Table 1 reports selected applications by using miniaturized techniques [26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44].…”

Section: Selected Applications Of Miniaturized Techniques Used For Th...mentioning

confidence: 99%

Potentiality of miniaturized techniques for the analysis of drugs of abuse

et al. 2021

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“…CE provides excellent performance in clinical analysis as well [102], because it can deliver high separation power, is highly versatile in its implementation, and consume small sample amounts. For example, a portable CE analyzer was employed for detection of abused drugs in oral fluid [103], and HF-LLME or microwave-assisted extraction (MAE) was coupled with CE for drug analysis in dried blood spot (DBS) samples [24104]. No doubt CE will remain a highly effective tool for study of biomolecules in cells, tissues, or other biological samples, as well as for high-throughput analysis of drugs and their metabolites in body fluids.…”

Section: Conclusion and Outlooksmentioning

confidence: 99%

Recent (2018–2020) development in capillary electrophoresis

Gao

Zhong

2021

Anal Bioanal Chem

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Development of new capillary electrophoresis (CE) methodology and instrumentation, as well as application of CE to solve new problems, remains an active research area because of the attractive features of CE compared to other separation techniques. In this review, we focus on the representative works about sample preconcentration, separation media or capillary coating development, detector construction, and multidimensional separation in CE, which are judiciously selected from the papers published in 2018-2020.

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“…To control these drugs (such as amphetamines and heroin) effectively, it is necessary to develop selective and sensitive analytical methods suitable for their unambiguous identification and determination in illicit samples and biological matrices. This has traditionally been carried out using gas chromatography-mass spectrometry (GC-MS) [ 1 , 2 ], high-performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MSMS) [ 3 , 4 ], capillary electrophoresis (CE) [ 5 , 6 ], immunosensor [ 7 ] and immunoassay [ 8 ], etc. In recent years, the mixing use of drugs is becoming one of the epidemiological characteristics of drug abuse patterns [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…In this context, CE provides original characteristics in terms of separation mechanisms, rapid and efficient analysis with remarkably high resolution, small sample volume, high sample throughput, low operational cost and tolerance to biological matrices [ 10 ]. All these characteristics ideally make it an attractive technique in comparison with liquid chromatography (LC) and gas chromatography (GC) for forensic toxicological analysis [ 5 , 6 ]. However, poor concentration sensitivity of traditional CE with on-column UV detection due to low sample injection volume and short optical path length limit the use of CE as an effective method to determine trace analytes in biological and environmental samples [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Determination of multiple drugs of abuse in human urine using dispersive liquid–liquid microextraction and capillary electrophoresis with PDA detection

Meng

et al. 2021

Forensic Sciences Research

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A new method was developed for pre-concentration and determination of multiple drugs of abuse in human urine using dispersive liquid–liquid microextraction (DLLME) and capillary electrophoresis (CE) with photodiode array detection. The method was based on the formation of tiny droplets of an organic extractant in the prepared sample solution using water-immiscible organic solvent (chloroform) dissolved in water-miscible organic dispersive solvent (isopropyl alcohol). The organic phase, which extracted eight drugs of abuse from the prepared urine solution, was separated by centrifugation. The sedimented phase was transferred into a small volume CE auto-sampler vial with 10 µL of 1% HCl methanol solution and evaporated to dryness. The residue was reconstituted in lidocaine hydrochloride (internal standard) aqueous solution and introduced by electrokinetic injection into CE. Under the optimum conditions, acceptable linear relationship was observed in the range of 3.0–500 ng/mL with the correlation coefficient ( r ) of 0.9982–0.9994 for spiked urine samples. The limit of detection (LOD) (S/N = 3) was estimated to be 1.0 ng/mL. A recovery of 75.7%–90.6% was obtained for spiked samples. The mean relative error (MRE) was within ±7.0% and the relative standard deviation (RSD) was less than 6.9%. The proposed DLLME-CE procedure offers an alternative analytical approach for the sensitive detection of drugs of abuse in real urine samples. Key points The dispersive liquid-liquid microextraction (DLLME) was involved for the determination of drugs in urine with capillary electrophoresis with photodiode array detection (CE-PDA). Good linearity, sensitivity, recovery and precision were achieved. The proposed method was eco-friendly with microliter scale solvent consumption.

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Use of a newly-developed portable capillary electrophoresis analyser to detect drugs of abuse in oral fluid: A case study

Cited by 17 publications

References 23 publications

Potentiality of miniaturized techniques for the analysis of drugs of abuse

Potentiality of miniaturized techniques for the analysis of drugs of abuse

Recent (2018–2020) development in capillary electrophoresis

Determination of multiple drugs of abuse in human urine using dispersive liquid–liquid microextraction and capillary electrophoresis with PDA detection

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