Use of a new Trichoderma harzianum strain isolated from the Amazon rainforest with pretreated sugar cane bagasse for on-site cellulase production

Delabona, Priscila da Silva; Farinas, Cristiane S.; Silva, Mateus Ribeiro da; Freitas, Sindélia; Pradella, José Geraldo da Cruz

doi:10.1016/j.biortech.2011.12.048

Cited by 143 publications

(80 citation statements)

References 14 publications

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“…This would also be expected during hydrolysis where the absence of lignin probably reduces the adsorption of cellulolytic enzymes onto the lignin fraction of biomass (Berlin et al 2005). This behavior was also reported for a T. harzianum strain isolated from the Amazon rainforest grown on pretreated sugarcane bagasse (Delabona et al 2012).…”

Section: Discussionsupporting

confidence: 76%

Obtaining New Cultures Of Microorganisms That Produces Cellulases And Xylanases From The Sugarcane Bagasse

Gikonyo¹

2015

Sugarcane as Biofuel Feedstock

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Brazil is a major producer of agro-industrial residues, such as sugarcane bagasse, which could be used as raw material for microbial production of cellulases as an important strategy for the development of sustainable processes of second generation ethanol production. For this purpose, this work aimed at screening for glycosyl hydrolase activities of fungal strains isolated from the Brazilian Cerrado. Among 13 isolates, a Trichoderma harzianum strain (L04) was identified as a promising candidate for cellulase production when cultured on in natura sugarcane bagasse. Strain L04 revealed a well-balanced cellulolytic complex, presenting fast kinetic production of endoglucanases, exoglucanases and β-glucosidases, achieving 4,022, U.L -1 (72 h), 1,228 U.L -1 (120 h) and 1,968 U.L -1 (48 h) as the highest activities, respectively. About 60% glucose yields were obtained from sugarcane bagasse after 18 hours hydrolysis. This new strain represents a potential candidate for on-site enzyme production using sugarcane bagasse as carbon source.

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Section: Discussionsupporting

confidence: 76%

Obtaining New Cultures Of Microorganisms That Produces Cellulases And Xylanases From The Sugarcane Bagasse

Gikonyo¹

2015

Sugarcane as Biofuel Feedstock

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“…This strain was isolated from the Amazon forest reserve of Embrapa, Belém -PA, Brazil and grown on potato dextrose agar plates (Difco TM , USA) at 29 C for 7 days (Delabona et al, 2012).…”

Section: Production Of Fermented Product Of Trichoderma Harzianum P49p11mentioning

confidence: 99%

“…A new species of filamentous fungus, Trichoderma harzianum, isolated in the Amazon, has shown the potential to hydrolyze biomass in ways similar to the Trichoderma reesei fungus (Delabona et al, 2012), which is one of the most studied and utilized fungi for the hydrolysis of biomass. T. harzianum was studied and used for phytopathogenic biocontrol, but recently it has been studied as a hydrolysis agent for the production of 2G ethanol (Thrane et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

ETHANOL PRECIPITATION OF GLYCOSYL HYDROLASES PRODUCED BY Trichoderma harzianum P49P11

Mariño

Freitas

Miranda

2015

Braz. J. Chem. Eng.

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-This study aimed to concentrate glycosyl hydrolases produced by Trichoderma harzianum P49P11 by ethanol precipitation. The variables tested besides ethanol concentration were temperature and pH. The precipitation with 90% (v/v) ethanol at pH 5.0 recovered more than 98% of the xylanase activity, regardless of the temperature (5.0, 15.0, or 25.0 °C). The maximum recovery of cellulase activity as FPase was 77% by precipitation carried out at this same pH and ethanol concentration but at 5.0 °C. Therefore, ethanol precipitation can be considered to be an efficient technique for xylanase concentration and, to a certain extent, also for the cellulase complex.

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“…The same soluble carbon source was also reported as the best inducer for the production of cellulases. Delabona et al (2012) used Trichoderma harzianum strain grown in Erlenmeyer flasks with liquid medium at 29°C, and reported that the use of sucrose cause a higher activity of ) than lactose at the same concentration. Lee et al (2010) tested culture media with different combinations of nitrogen sources and soluble sugars and observed increased production of CMCase in medium supplemented with sucrose using a strain of Bacillus subtilis.…”

Section: Different Soluble Sugars In Fermentation Media For Cellulasementioning

confidence: 99%

“…Singhania et al (2010) stated that, although the relationship between productivity and morphology of the fungus is not yet clearly established, the behavior of the microorganism may vary with changing components of the medium hindering the adherence of cells to the culture medium and consequently, the production of metabolites. In a study evaluating the influence of nutritional conditions on the morphology of T. reesei RUT C30 on cellulose production, Domingues et al (2000) observed the formation of pellets in the medium with lactose as the carbon source and lower FPase production due to this behavior of the fungus. On the other hand, in the same culture medium added with Tween 80, without pellets, there was an increased FPase production within 72h.…”

Section: Different Soluble Sugars In Fermentation Media For Cellulasementioning

confidence: 99%

<b>Strategies to increase cellulase production with submerged fermentation using fungi isolated from the Brazilian biome

Faheina

Amorim

Souza

et al. 2015

Acta Sci. Biol. Sci.

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ABSTRACT. Studies on new microbial sources of cellulase and accurate assessment of the steps that increase cellulase production are essential strategies to reduce costs of various processes using such enzymes. This study aimed at the selection of cellulase-producing filamentous fungi, and at the research of parameters involving cellulase production by submerged fermentation. The first test consisted of selecting the best cellulase-producing microorganisms (FPase) in Erlenmeyer flasks containing 200 mL of specific growth medium. The next test was designed to further investigate the enzyme production in fermentation with four types of soluble sugars: glucose, lactose, sucrose and xylose. In bioreactor tests, three different inoculation strategies were analyzed. The best FPase activity was presented by the strain Trichoderma sp. ). The best inoculation strategy for the bioreactor was a spore suspension obtained from a semi-solid state fermentation of wheat bran for 72h.Keywords: cellulolytic enzymes, enzyme induction.Estratégias para produção de celulases através de fermentação submersa utilizando fungos isolados do bioma brasileiro RESUMO. Estudos sobre novas fontes microbianas e análises mais acuradas das etapas que compõem a produção de celulases são essenciais como estratégias para diminuir os custos gerados pelo uso de celulases nos processos de obtenção de açúcares fermentescíveis. O trabalho teve como objetivo a seleção de fungos filamentosos produtores de celulases e a investigação de parâmetros que envolvem a produção enzimática em fermentação submersa. O primeiro teste consistiu em selecionar os melhores fungos produtores de celulases totais em frascos Erlenmeyer contendo 200 mL de meio de cultura específico. O teste subsequente teve o intuito de investigar a produção enzimática com quatro tipos de açúcares solúveis: glicose, lactose, sacarose e xilose. Nos testes em biorreator foram analisados três diferentes estratégias de inoculação. Na etapa de seleção a melhor atividade de FPase foi apresentada por Trichoderma sp. CMIAT 041 (49,9 FPU L ). A melhor estratégia de inoculação foi a suspensão de esporos obtidos a partir de fermentação em farelo de trigo, no tempo 72h.Palavras-chave: enzimas celulolíticas, indução enzimática.

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Use of a new Trichoderma harzianum strain isolated from the Amazon rainforest with pretreated sugar cane bagasse for on-site cellulase production

Cited by 143 publications

References 14 publications

Obtaining New Cultures Of Microorganisms That Produces Cellulases And Xylanases From The Sugarcane Bagasse

Obtaining New Cultures Of Microorganisms That Produces Cellulases And Xylanases From The Sugarcane Bagasse

ETHANOL PRECIPITATION OF GLYCOSYL HYDROLASES PRODUCED BY Trichoderma harzianum P49P11

<b>Strategies to increase cellulase production with submerged fermentation using fungi isolated from the Brazilian biome

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