Use of a new adsorbent in the separation and detection of glucose and fructose by HPTLC

Klaus, R.; Fischer, W.; Hauck, H. E.

doi:10.1007/bf02261015

Cited by 29 publications

(8 citation statements)

References 4 publications

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“…However, for fluorescence measurements, on the basis of the selective derivatization of sucralose, this interference was not observed. For absorbance measurements, LOD has been reported to be 1 mg/L in the matrix and 5 ng/zone in the HPTLC plate (16). For fluorescence measurements, similar limits were established.…”

Section: Resultsmentioning

confidence: 93%

“…Also, capillary zone electrophoresis (CE) has been used with indirect ultraviolet absorption at 238 nm in a 3,5-dinitrobenzoic acid buffer (13,14) but shows shifts in migration times and interferences with fructose or other sugars if these are present in higher amounts. A new planar chromatographic method for sucralose (15) employed the reagent-free derivatization on amino phases by just heating the plate after chromatography, an elegant way of derivatization well known for the analysis of sugars (16). The mechanism is supposed to be a Maillard reaction forming high molecular mass derivatives of fluorescent nature (17).…”

Section: Introductionmentioning

confidence: 99%

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Analysis and Stability of Sucralose in a Milk-Based Confection by a Simple Planar Chromatographic Method

Morlock

Prabha

2007

J. Agric. Food Chem.

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Sucralose used as high potency sweetener in foods was determined in burfi, a milk-based confection produced in-house. Therefore planar chromatography was employed as a preferred method because of a reagent-free derivatization step. Sucralose was determined on HPTLC amino plates whose amino groups reacted with sucralose to fluorescent zones by just heating the plate after chromatography. Thus derivatization was simultaneously performed for 22 separations per plate, and with ease, over 300 runs can be performed within a day of labor. The within-run precision (%RSD) of sucralose determination in milk-based confection was 4.2% (n = 5), and the mean recovery 88% +/- 4.7% (n = 6). LOD via fluorescence measurement was 6 ng/band for standard solutions and 1 mg/kg for the milk-based matrix. According to European legislation, the limits for sucralose addition ranged between 10 and 3000 mg/kg for various foods and thus were fully met with this method. The fluorescence measurement at 366/>400 nm turned out to be slightly more robust and intense than the absorbance measurement at UV 254 nm. The stability of sucralose in milk-based confection was proved under the usual storage conditions at 5, 30, and 45 degrees C for up to 28 days. Potential hydrolysis products of sucralose caused by various modes of storing the confection were not observed up to 28 days.

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Section: Resultsmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Analysis and Stability of Sucralose in a Milk-Based Confection by a Simple Planar Chromatographic Method

Morlock

Prabha

2007

J. Agric. Food Chem.

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“…Chromatographic properties of amino-silica gel layers are similar to those of nonmodified silica gel, and some identical solvent systems can be used with both sorbents ( Table 6). The main advantage of amino-bonded silica is that it affords simple detection of separated sugars by a thermal in situ reaction (56)(57)(58)(59). Sugars are readily converted, leading to stable, intensely fluorescing derivatives.…”

Section: Aminopropyl-bonded Silicamentioning

confidence: 99%

Carbohydrates

Maloney¹

2003

Handbook of Thin-Layer Chromatography

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“…[18] A simple and effective method was developed for fluorometric detection of glucose, fructose, and other carbonylic compounds after TLC separation on amino-silica gel. [19] A reagent-free derivatisation procedure was also successfully applied for fluorescence induction in various carbohydrates, [20] uric acid, and creatinine [21] or catecholamines. [22] Therefore, the aim of this paper was to develop a simple TLC-based method with reagent-free derivatisation, suitable for routine analysis of sterigmatocystin at levels of interest in the lower mg/kg range.…”

Section: Stroka Et Al 2102mentioning

confidence: 99%

Determination of the Mycotoxin, Sterigmatocystin, by Thin‐Layer Chromatography and Reagent‐Free Derivatisation

Stroka

Daško

Spangenberg

et al. 2004

Journal of Liquid Chromatography & Related Technologies

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This paper describes a thin-layer chromatographic (TLC) method, which allows the determination of sterigmatocystin at a level of 2 mg/kg in various cereal grains of interest. Sterigmatocystin is extracted from the food matrix and further purified by phenyl-bond solid-phase extraction (SPE). The 2101 separation and identification is performed on an amino-derivatised highperformance TLC (HP-TLC) plate. The derivatisation for densitometric measurement and visual inspection is achieved reagent free by heating the plate. Sterigmatocystin results in highly fluorescent spots. The method has shown good recovery values for the various cereals analysed (e.g., around 80% for wheat). The method was applied for monitoring the sterigmatocystin content in 85 cereal samples, purchased from the local (Italian) market in 2002. However, none of the samples was found to be positive, indicating that this mycotoxin was not a problematic contaminant in products of this particular region and in this specific year.

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Use of a new adsorbent in the separation and detection of glucose and fructose by HPTLC

Cited by 29 publications

References 4 publications

Analysis and Stability of Sucralose in a Milk-Based Confection by a Simple Planar Chromatographic Method

Analysis and Stability of Sucralose in a Milk-Based Confection by a Simple Planar Chromatographic Method

Carbohydrates

Determination of the Mycotoxin, Sterigmatocystin, by Thin‐Layer Chromatography and Reagent‐Free Derivatisation

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