Use of a monoclonal antibody to estrone-3-glucuronide in an enzyme-linked immunosorbent assay (ELISA)

Elder, Peter A.; Manley, Laurette; Lewis, John G.

doi:10.1016/0022-4731(90)90085-7

Cited by 14 publications

(7 citation statements)

References 17 publications

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“…Most of the competitive assays use either enzyme-or substratelabeled steroid tracers. These assays often lack analytical sensitivity and specificity due to an altered structure of the respective steroid tracers (5)(6)(7)(8)(9)(10). This effect is observed in cases in which steroids have been derivatized at the ring positions 3 or 17.…”

Section: Introductionmentioning

confidence: 99%

“…NMR (400 MHz, CDC13, ppm): 8.13-6.74 (m, 13H, 1-CH, 2-CH, 4-CH, 3-benzoxy-H, 6-benzamide arómate H); 6.32 (d, 1H, Jnh-6 = 9 Hz, NH); 5.52 (m, 1H); 4.69 (t, 1H, J = 8 Hz, 17-C-H); 2.06 (s, 3H, 17-OCOCH3); 0.84 (s, 3H, 18-CH). NMR (400 MHz, CD3OD, ppm): 7.98-6.62 (m, 8H, 1-CH, 2-CH, 4-CH, 6-benzamide arómate H); 5.39 (dd, 1H, Je-tax = 11 Hz, Je-7eq = 7 Hz, 6/8-CH); 4.68 (t, 1H, J = 8 Hz, 17-CH); 2.04 (s, 3H, 17-OCOCHg); 0.90 (s, 3H, 18-CH). UV: 277 nm, log e = 2.64; 233 nm, log e = 3.33.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of 3-Hydroxyestra-1,3,5(10)-trien-17-one and Estra-1,3,5(10)-triene-3,17β-diol 6α-N-(iε-Biotinyl)caproamide, Tracer Substances for Developing Immunoassays for Estrone and Estradiol

Luppa¹,

Birkmayer²,

Hauptmann³

1994

Bioconjugate Chem.

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We describe the synthesis of 3-hydroxyestra-1,3,5(10)-trien-17-one 6 alpha-N-(epsilon-biotinyl)caproamide and 3,17 beta-dihydroxyestra-1,3,5(10)-triene 6 alpha-N-(epsilon-biotinyl) caproamide from 3-hydroxyestra-1,3,5(10)-trien-17-one and 3,17 beta-dihydroxyestra-1,3,5(10)-triene, via the 6-keto estrogenic derivatives. The reductive amination of these compounds is an effective step toward an epimeric mixture of the respective amines, which are easily biotinylated by use of N-(epsilon-biotinylcaproyl)-N- hydroxysuccinimide ester. The 6 alpha-epimers could be isolated from the alpha/beta-composition by application of isocratic HPLC, and overall yields were about 20% for the epimeric end products. The structures of the stereoisomers could clearly be assigned through 1H NMR studies. The ratios of the respective isomers obtained from the reductive amination were found to be 3(alpha):2(beta). The biotinylated estrogens can be used as tracers in a novel immunoassay concept for the determination of these analytes in human serum. Ring position 6 was selected for derivatization because of its distance from the functionalized positions 3 and 17 and, therefore, of a negligible alteration of the tracer's structure in comparison to underivatized estrone or estradiol.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synthesis of 3-Hydroxyestra-1,3,5(10)-trien-17-one and Estra-1,3,5(10)-triene-3,17β-diol 6α-N-(iε-Biotinyl)caproamide, Tracer Substances for Developing Immunoassays for Estrone and Estradiol

Luppa¹,

Birkmayer²,

Hauptmann³

1994

Bioconjugate Chem.

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“…Instead it makes use of enzyme-labelled second antibody, which is not only easily available from commercial sources but is also less expensive and has a long shelf life. This label can be used as a common reagent for steroid and protein hormone assays based on the immobilized antigen approach (8,9). The developed indirect ELISA is much more sensitive than the reported ELISA based on the same principle (10).…”

Section: Discussionmentioning

confidence: 99%

An Indirect ELISA for Urinary Gonadotropins Using Immobilized Human Menopausal Gonadotropin

Khatkhatay

Desai²,

Sankolli³

et al. 1992

Clinical Chemistry and Laboratory Medicine

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Summary: An indirect ELISA for the estimation of urinary gonadotropins is described. Human menopausal gonadotropin is adsorbed on a microtitre plate, where it serves as an immunosorbent. The residual antigonadotropin antibody is captured by the immunosorbent after reaction with the sample or standard and detected with enzyme-labelled antispecies antibody (antirabbit γ-globulin-horse radish peroxidase). The assay developed here is rapid and satisfies usual validatory criteria expected from an immunoassay. Moreover, it obviates the need for extraction of samples with acetone, as shown by the close aggreement between the respective lutropin or follitropin concentrations in extracted and unextracted urine samples.

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“…The method used to locate the centre of a pregnanediol peak is shown in Fig Ovarian failure is associated with a persistent eleva¬ tion in the urinary excretion of the pituitary hormones follicle-stimulating hormone (FSH) and luteinizing hormone (LH), to levels above 0-8 IU creatinine/ mmol (Metcalf & Livesey, 1979), together with a persistent depression in urinary oestrogen excre¬ tion below 5 nmol/mmol creatinine (Rae, Mole & Paterson, 1988). To monitor ovarian dysfunction the excretion of oestrone-3-glucuronide was measured by an enzyme-linked immunosorbent assay (Elder, Manley & Lewis, 1990) and LH by the LKB Delphia immunofluorometric assay (Pharmacia Diagnostics, 1989). Interassay coefficients of variation for the oestrone assay were 6-8% and for the LH assay 3-2-8-6%.…”

Section: Methodsmentioning

confidence: 99%

Retention of normal ovarian function after hysterectomy

Metcalf¹,

Braiden²,

Livesey³

1992

Journal of Endocrinology

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What are the long-term effects of hysterectomy on the ovaries of normal women? Ninety-three women aged 29-44 years (median, 38 years) who had undergone hysterectomy for benign reasons 0.3-9.1 years prior to investigation, contributed urine samples twice weekly for a period of 53-149 days (median 102 days) for pregnanediol analysis. The interval between successive pregnanediol peaks and their increment over baseline were measured. The median peak interval was 27.3 days, and 93.3% of all intervals were of 21- to 35-days duration. Of the 337 peaks observed, 96.7% met the criteria previously used to define an ovulatory cycle. These are similar to the figures reported for menstruant women of comparable age. ANOVA showed no significant effect of age or time since hysterectomy on either the interval between peaks or peak increment (P > 0.10 in all cases). The evidence suggests that the ovaries of women who have no uterus behave like those of intact women.

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Use of a monoclonal antibody to estrone-3-glucuronide in an enzyme-linked immunosorbent assay (ELISA)

Cited by 14 publications

References 17 publications

Synthesis of 3-Hydroxyestra-1,3,5(10)-trien-17-one and Estra-1,3,5(10)-triene-3,17β-diol 6α-N-(iε-Biotinyl)caproamide, Tracer Substances for Developing Immunoassays for Estrone and Estradiol

Synthesis of 3-Hydroxyestra-1,3,5(10)-trien-17-one and Estra-1,3,5(10)-triene-3,17β-diol 6α-N-(iε-Biotinyl)caproamide, Tracer Substances for Developing Immunoassays for Estrone and Estradiol

An Indirect ELISA for Urinary Gonadotropins Using Immobilized Human Menopausal Gonadotropin

Retention of normal ovarian function after hysterectomy

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