Use of a molecularly cloned human SS.B antigen to detect anti-SS.B antibodies

Owe-Young, R; Horn, Sheila; Edmonds, John; Sturgess, A.

doi:10.1016/0896-8411(92)90148-j

Cited by 3 publications

(2 citation statements)

References 27 publications

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“…The second conclusion is that the use of synthetic peptides as substrates for the identification of anti-La/SSB antibodies is a promising method. In fact, the positivity rate in healthy blood donors is low, comparable to that observed in assays based on recombinant La/SSB [38]. Furthermore, disease control sera (i.e.…”

mentioning

confidence: 71%

Fine specificity of autoantibodies to La/SSB: epitope mapping, and characterization

Tzioufas

Yiannaki

Sakarellos‐Daitsiotis

et al. 1997

Clinical and Experimental Immunology

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SUMMARYThe B cell epitope mapping of La/SSB was performed using 20 mer synthetic peptides overlapping by eight amino acids covering the whole sequence of the protein. IgG, purified from sera of five patients with systemic lupus erythematosus (SLE) and four sera from patients with primary Sjögren's syndrome (pSS) were tested against the overlapping synthetic peptides. Peptides highly reactive with purified IgG were those spanning the regions 145-164, 289-308, 301-320 and 349-368 364 . Predicted features and molecular similarities of the defined epitopes were investigated using protein databases. The La epitope 147 HKAFKGSI 154 presented 83·3% similarity with the 139 HKGFKGVD 146 region of human myelin basic protein (MBP) and 72% similarity with the fragment YKNFKGTI of human DNA topoisomerase II. Peptides corresponding to these sequences cross-reacted with anti-La/SSB antibodies. Sixty-three sera with anti-La/SSB antibodies from patients with pSS or SLE, 35 sera without anti-La/SSB antibodies from patients with SS or SLE and 41 sera from age/sex-matched healthy blood donors were tested against biotinylated synthetic epitope analogues in order to determine their sensitivity and specificity for the detection of anti-La/SSB antibodies. Anti-La/SSB were detected with various frequencies ranging from 20% to epitope 147 HKAFKGSI 154 to 100% to epitope 349 GSGKGKVQGKKTKF 364 . The overall sensitivity and specificity using all assays with the synthetic peptides were found to be 93·6% and 85·6%, respectively. In conclusion, antibodies to La/SSB constitute a heterogeneous population, directed against different linear B cell epitopes of the molecule. The epitope 147 HKAFKGSI 154 presents molecular similarity with fragments of two other autoantigens, i.e. human MBP and DNA topoisomerase II. Finally, synthetic epitope analogues exhibit high sensitivity and specificity for the detection of anti-La/SSB antibodies.

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mentioning

confidence: 71%

Fine specificity of autoantibodies to La/SSB: epitope mapping, and characterization

Tzioufas

Yiannaki

Sakarellos‐Daitsiotis

et al. 1997

Clinical and Experimental Immunology

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“…rabbit thymus) [24,37,75], This, however, has not been the case for the use of recombinant SS-A/Ro anti gen. Low sensitivity and/or specificity are the general rule for the assays, indicating the pressing need for the elucida tion of both the epitopes recognized by autoimmune sera, and the possible modifications that these epitopes might un dergo as a result of their expression from prokaryotic sourc es. possesses a multitude of functional characteristics, which include (I) proteolytic activity (e.g.…”

Section: Ss-a/ro and Ss-b/la Ribonucleoproteinsmentioning

confidence: 99%

Use of Recombinant Proteins in the Diagnosis of Autoimmune Connective Tissue Diseases

Galperin

Coppel

Gershwin³

1996

Int Arch Allergy Immunol

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The use of recombinant proteins has been an invaluable tool for the study of the structure and function of cell components, and the analysis of autoimmune-mediated responses. Furthermore, it has also been instrumental in facilitating the development of reliable assays for the detection of a number of autoantibodies. This article discusses basic aspects of recombinant DNA technology, as well as its applications and limitations in the diagnosis of autoimmune connective tissue diseases such as systemic lupus erythematosus, Sjögren’s syndrome, systemic scleroderma, mixed connective tissue disease, autoimmune inflammatory myopathies and primary vasculitides.

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Relationship Between Heat Shock Protein Induction and the Binding of Antibodies to the Extractable Nuclear Antigens on Cultured Human Keratinocytes

Furukawa

Ikai

Matsuyoshi

et al. 1993

Journal of Investigative Dermatology

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Use of a molecularly cloned human SS.B antigen to detect anti-SS.B antibodies

Cited by 3 publications

References 27 publications

Fine specificity of autoantibodies to La/SSB: epitope mapping, and characterization

Fine specificity of autoantibodies to La/SSB: epitope mapping, and characterization

Use of Recombinant Proteins in the Diagnosis of Autoimmune Connective Tissue Diseases

Relationship Between Heat Shock Protein Induction and the Binding of Antibodies to the Extractable Nuclear Antigens on Cultured Human Keratinocytes

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