Use of a mixture of sand and water-absorbent synthetic polymer as substrate for the xenic culturing of plant-parasitic nematodes in the laboratory

Réversat, Georges; Boyer, Johnny; Sannier, Christianne; Pando‐Bahuon, Anne

doi:10.1163/156854199508027

Cited by 85 publications

(31 citation statements)

References 6 publications

(4 reference statements)

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“…Seeds were first pre-germinated in petri dishes at 30 °C for 4 days in dark. Each germinated seedling was planted into a specially made polyvinylchloride tube containing sand and absorbent polymer (Reverstat et al 1999). Then the seedlings were grown in a rice culture room under controlled environmental conditions (26/24 °C day/night temperature, 70% relative humidity, 12/12 h light/dark cycle).…”

Section: Methodsmentioning

confidence: 99%

QTL-seq reveals a major root-knot nematode resistance locus on chromosome 11 in rice (Oryza sativa L.)

et al. 2019

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The root-knot nematode Meloidogyne graminicola is a serious pest in rice affecting production in many rice growing areas. Natural host resistance is an attractive control strategy because the speed of the parasite’s life cycle and the broad host range it attacks make other control measures challenging. Although resistance has been found in the domesticated African rice Oryza glaberrima and the wild rice species O. longistaminata, the introgression of resistance genes to Asian rice O. sativa is challenging. Resistance due to a major gene in O. sativa would greatly aid breeding. Recently two accessions resistant to M. graminicola have been identified in a screen of 332 diverse O. sativa cultivars. In this study, these two resistant cultivars, LD 24 (an indica from Sri Lanka) and Khao Pahk Maw (an aus from Thailand), were crossed with a moderately susceptible cultivar, Vialone Nano (a temperate japonica from Italy). Approximately 175 F 2 progeny of both populations were screened for susceptibility to M. graminicola infection. Between 20 and 23 individuals with highest and lowest galls per plants were pooled to make susceptible and resistant bulks which were sequenced to conduct bulked segregant analysis using the QTL-seq method. This revealed a nematode resistance locus from 23 Mbp to the bottom of rice chromosome 11 in both crosses suggesting a rare introgression of the same locus is responsible for resistance in both cultivars. While this information can be used in marker-assisted breeding, analysis of available SNP data revealed candidate loci and genes worthy of further investigation for gene identification. Electronic supplementary material The online version of this article (10.1007/s10681-019-2427-0) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

QTL-seq reveals a major root-knot nematode resistance locus on chromosome 11 in rice (Oryza sativa L.)

et al. 2019

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“…Transgenic OsEin2b RNAi and NahG lines were kindly provided by Yinong Yang (Pennsylvania State University); the hebiba mutant and its corresponding wild-type cv Nihonmasari were kindly provided by P. Nick (Karlsruhe University). Seeds were germinated on wet filter paper for 5 d at 30°C, transferred to Synthetic Absorbent Polymer substrate (Reversat et al, 1999), and further grown at 26°C under a 12-h/12-h light regime (150 mmol m 22 s 21 ) and 70% to 75% relative humidity. Plants were watered with distilled water twice per week and fertilized once per week with 10 mL of Hoagland solution.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

The Jasmonate Pathway Is a Key Player in Systemically Induced Defense against Root Knot Nematodes in Rice

et al. 2011

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Complex defense signaling pathways, controlled by different hormones, are involved in the reaction of plants to a wide range of biotic and abiotic stress factors. We studied the ability of salicylic acid, jasmonate (JA), and ethylene (ET) to induce systemic defense in rice (Oryza sativa) against the root knot nematode Meloidogyne graminicola. Exogenous ET (ethephon) and JA (methyl jasmonate) supply on the shoots induced a strong systemic defense response in the roots, exemplified by a major up-regulation of pathogenesis-related genes OsPR1a and OsPR1b, while the salicylic acid analog BTH (benzo-1,2,3-thiadiazole-7-carbothioic acid S-methyl ester) was a less potent systemic defense inducer from shoot to root. Experiments with JA biosynthesis mutants and ET-insensitive transgenics showed that ET-induced defense requires an intact JA pathway, while JA-induced defense was still functional when ET signaling was impaired. Pharmacological inhibition of JA and ET biosynthesis confirmed that JA biosynthesis is needed for ET-induced systemic defense, and quantitative real-time reverse transcription-polymerase chain reaction data revealed that ET application onto the shoots strongly activates JA biosynthesis and signaling genes in the roots. All data provided in this study point to the JA pathway to play a pivotal role in rice defense against root knot nematodes. The expression of defense-related genes was monitored in root galls caused by M. graminicola. Different analyzed defense genes were attenuated in root galls caused by the nematode at early time points after infection. However, when the exogenous defense inducers ethephon and methyl jasmonate were supplied to the plant, the nematode was less effective in counteracting root defense pathways, hence making the plant more resistant to nematode infection.

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“…This is a transgenic rice whose OsNPR1 functional gene version controls the transcription of plant defense gene and it is responsive to Riboflavin (Dong, H and Beer, S.V 2000); (5) Nihonmasari rice was used as a control for Hebiba rice. (Reversat et al, 1999) was used. PVC (polymer, polyvinylchloride) tubes were filled with SAP-substrates, rice plantlets were allowed to grow on and hoagland solution was added to the seedlings as source of nutrients.…”

Section: Methodsmentioning

confidence: 99%

Riboflavin induced tolerance against the root-knot nematode <i>Melopidogyne graminicola</i> in rice

Nsengimana¹,

Ateka²,

Nsabimana³

et al. 2018

RWJour

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The role of plant hormones such as Salicylic acid, Jasmonic acid and Ethylene in plant defense has been largely investigated particularly in dicotyledonous plants. Riboflavin has also shown effects on plant physiology but its role in plant defense/tolerance is yet to be elucidated.Rice plantlets were randomly grown in laboratory, sprayed with hormones and riboflavin 24 hours before their infection by the nematodes meloidogyne gramicola juvenile 2. Two types of infection experiments were done. The normal rice nipponbare susceptible to M. gramincola infection was used in the first while in the second series experiments four transgenics/mutants were used. The shoots length, roots length and the roots weight were measured. The numbers of galls per plant were counted under a stereomicroscope. The relative expression of the genes Osein2b, Oswrky 45, OsJAmyb and OsNPR1 was evaluated using the qRT-PCR method.It was noticed that the plantlets treated with BTH significantly developed less galls compared to control (P = 0.000<0.05). This was also observed with ETHEPHON and MeJA spray (P =

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Use of a mixture of sand and water-absorbent synthetic polymer as substrate for the xenic culturing of plant-parasitic nematodes in the laboratory

Cited by 85 publications

References 6 publications

QTL-seq reveals a major root-knot nematode resistance locus on chromosome 11 in rice (Oryza sativa L.)

QTL-seq reveals a major root-knot nematode resistance locus on chromosome 11 in rice (Oryza sativa L.)

The Jasmonate Pathway Is a Key Player in Systemically Induced Defense against Root Knot Nematodes in Rice

Riboflavin induced tolerance against the root-knot nematode <i>Melopidogyne graminicola</i> in rice

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