Use of a microelectrode array to record extracellular pacemaker potentials from the gastrointestinal tracts of the ICR mouse and house musk shrew (Suncus murinus)

Liu, Julia Y.H.; Du, Peng; Chan, Wood Yee; Rudd, John A.

doi:10.1016/j.ceca.2019.05.002

Cited by 12 publications

(18 citation statements)

References 45 publications

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“…Responses are plotted as median with individual values plotted. Friedman's test/Dunn's test comparing responses to incremental concentrations of atropine to the response to neostigmine in the presence of TTX; *P < 0.05 corpus ($6-8/min; Kim et al, 2003;Liu et al, 2019;Worth et al, 2015) and antrum ($3-4/min; Forrest et al, 2006;Hwang et al, 2016;Kim et al, 2003;Sanders, 2020), and also the contraction frequency in antrum circular muscle (3.6 ± 0.5 c.p.m. ;Hwang et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Evidence for tetrodotoxin‐resistant spontaneous myogenic contractions of mouse isolated stomach that are dependent on acetylcholine

Cai

Makwana

Straface

et al. 2021

British J Pharmacology

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Background and Purpose: Gastric pacemaker cells, interstitial cells of Cajal (ICC), are believed to initiate myogenic (non-neuronal) contractions. These become damaged in gastroparesis, associated with dysrhythmic electrical activity and nausea. We utilised mouse isolated stomach to model myogenic contractions and investigate their origin and actions of interstitial cells of Cajal modulators.Experimental Approach: Intraluminal pressure was recorded following distension with a physiological volume; tone, contraction amplitude and frequency were quantified. Compounds were bath applied.

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Section: Discussionmentioning

confidence: 99%

Evidence for tetrodotoxin‐resistant spontaneous myogenic contractions of mouse isolated stomach that are dependent on acetylcholine

Cai

Makwana

Straface

et al. 2021

British J Pharmacology

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“…Electrical recordings were performed using an MEA, as previously described ( Liu et al, 2019 ). In brief, intact full-thickness tissue segments of the antrum, duodenum, ileum and colon were isolated from the adult male shrews (55–75 g) and incubated in Kreb’s medium (in mM: NaCl, 115; KCl, 4.7; KH 2 PO 4 , 1.2; MgSO 4 .7H 2 O, 1.2; CaCl 2 .2H 2 O, 2.5; glucose, 10; NaHCO 3 , 25; sparged with 95%/5% oxygen/carbon dioxide.…”

Section: Methodsmentioning

confidence: 99%

“…Raw data were recorded using MC_Rack, V4.6.2 (Multichannel Systems, Germany) and imported into Spike 2 ® (Version 8.1, Cambridge Electronic Design, United Kingdom) for temporal analysis and into MATLAB (2016a, MathWorks) for spatial-temporal analysis, as previously described ( Liu et al, 2019 ). In brief, a second-order Butterworth lowpass IIR filter at 100 Hz was applied to the raw data, which was then down-sampled to 100 Hz.…”

Section: Methodsmentioning

confidence: 99%

“…The average period of waveforms was derived by averaging the periods of all waveforms (or peak events) that were identified. The propagating velocity was defined by the movement of the same peak event horizontally/vertically across the MEA, as previously described ( Liu et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

“…At the end of the experiments, the shrews were humanely sacrificed, and the antrum region of the stomach was isolated and processed to determine pathological changes, and immunohistochemistically stained to detect the presence of c-kit (a marker of ICC) and CD45 (a marker of inflammation). In addition, the effects of cisplatin on the spatiotemporal properties of slow-waves in isolated stomach antrum, duodenum, ileum and colon tissues were investigated using an MEA system ( Liu et al, 2019 ). Acute effects (within minutes) of cisplatin on gastrointestinal tissue was investigated, but an ex vivo sampling time of 90 min from animals treated with cisplatin was also used, to correspond to a timepoint where the most intense vagally-mediated emetic response in this species is observed ( Sam et al, 2003 ).…”

Section: Introductionmentioning

confidence: 99%

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Insights Into Acute and Delayed Cisplatin-Induced Emesis From a Microelectrode Array, Radiotelemetry and Whole-Body Plethysmography Study of Suncus murinus (House Musk Shrew)

Liu

et al. 2021

Front. Pharmacol.

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Purpose: Cancer patients receiving cisplatin therapy often experience side-effects such as nausea and emesis, but current anti-emetic regimens are suboptimal. Thus, to enable the development of efficacious anti-emetic treatments, the mechanisms of cisplatin-induced emesis must be determined. We therefore investigated these mechanisms in Suncus murinus, an insectivore that is capable of vomiting.Methods: We used a microelectrode array system to examine the effect of cisplatin on the spatiotemporal properties of slow waves in stomach antrum, duodenum, ileum and colon tissues isolated from S. murinus. In addition, we used a multi-wire radiotelemetry system to record conscious animals’ gastric myoelectric activity, core body temperature, blood pressure (BP) and heart rate viability over 96-h periods. Furthermore, we used whole-body plethysmography to simultaneously monitor animals’ respiratory activity. At the end of in vivo experiments, the stomach antrum was collected and immunohistochemistry was performed to identify c-Kit and cluster of differentiation 45 (CD45)-positive cells.Results: Our acute in vitro studies revealed that cisplatin (1–10 μM) treatment had acute region-dependent effects on pacemaking activity along the gastrointestinal tract, such that the stomach and colon responded oppositely to the duodenum and ileum. S. murinus treated with cisplatin for 90 min had a significantly lower dominant frequency (DF) in the ileum and a longer waveform period in the ileum and colon. Our 96-h recordings showed that cisplatin inhibited food and water intake and caused weight loss during the early and delayed phases. Moreover, cisplatin decreased the DF, increased the percentage power of bradygastria, and evoked a hypothermic response during the acute and delayed phases. Reductions in BP and respiratory rate were also observed. Finally, we demonstrated that treatment with cisplatin caused inflammation in the antrum of the stomach and reduced the density of the interstitial cells of Cajal (ICC).Conclusion: These studies indicate that cisplatin treatment of S. murinus disrupted ICC networking and viability and also affected general homeostatic mechanisms of the cardiovascular system and gastrointestinal tract. The effect on the gastrointestinal tract appeared to be region-specific. Further investigations are required to comprehensively understand these mechanistic effects of cisplatin and their relationship to emesis.

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Involvement of TRPV1 and TRPA1 in the modulation of pacemaker potentials in the mouse ileum

Liu

et al. 2021

Cell Calcium

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Use of a microelectrode array to record extracellular pacemaker potentials from the gastrointestinal tracts of the ICR mouse and house musk shrew (Suncus murinus)

Cited by 12 publications

References 45 publications

Evidence for tetrodotoxin‐resistant spontaneous myogenic contractions of mouse isolated stomach that are dependent on acetylcholine

Evidence for tetrodotoxin‐resistant spontaneous myogenic contractions of mouse isolated stomach that are dependent on acetylcholine

Insights Into Acute and Delayed Cisplatin-Induced Emesis From a Microelectrode Array, Radiotelemetry and Whole-Body Plethysmography Study of Suncus murinus (House Musk Shrew)

Involvement of TRPV1 and TRPA1 in the modulation of pacemaker potentials in the mouse ileum

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