Use of a low dose synthetic ACTH challenge test in normal and prednisone-treated dogs

Kemppainen, Robert J.; Thompson, F. N.; Lorenz, Michael D.

doi:10.1016/s0034-5288(18)32186-6

Cited by 36 publications

(40 citation statements)

References 4 publications

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“…2 The intra-and interassay coefficients of variation are 5.1% and 10.3%, respectively. Samples were allowed to clot for 20 minutes prior to centrifugation.…”

Section: Methodsmentioning

confidence: 98%

Effect of low doses of cosyntropin on serum cortisol concentrations in clinically normal dogs

Martin¹,

Behrend²,

Mealey³

et al. 2007

ajvr

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“…2 The intra-and interassay coefficients of variation are 5.1% and 10.3%, respectively. Samples were allowed to clot for 20 minutes prior to centrifugation.…”

Section: Methodsmentioning

confidence: 98%

Effect of low doses of cosyntropin on serum cortisol concentrations in clinically normal dogs

Martin¹,

Behrend²,

Mealey³

et al. 2007

ajvr

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“…Measurement of ADH as arginine vasopressin was determined using a radioimmunoassay kit (Nichols Institute, Diagnostics BV) as previously described (12, 13). For measurement of total cortisol concentration, assay was performed using a previously validated (14) radioimmunoassay kit (Coat-A-Count Cortisol Assay, DPC, Los Angeles, CA). The sensitivity of the assay was 14 nmol/L; all measurements below the standard curve were recorded as 7 nmol/L.…”

Section: Methodsmentioning

confidence: 99%

Defining normal adrenal function testing in the intensive care unit setting: A canine study*

Sweeney

Natanson²,

Banks³

et al. 2010

Critical Care Medicine

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Objective It is routine in intensive care medicine (ICM) to measure hypothalamic-pituitary-adrenal (HPA) function, commonly utilizing the ACTH stimulation test to diagnose absolute or relative adrenal insufficiency (RAI). It is therefore important to determine whether ICM therapies and testing themselves influence HPA test results. Design Prospective, 96 h animal study. Setting Research laboratory. Subjects 24 healthy canines. Interventions Animals were randomized into two groups—awake and unrestrained or treated with intensive care medicine therapies including sedation, intubation and mechanical ventilation (SIM). Animals were further randomized to receive dexamethasone (or placebo) or undergo either a total of 4 or 7 ACTH stimulation tests over 96 h. Measurements and Main Results SIM transiently increased both basal and post-ACTH total and free cortisol concentrations greater than two-fold as compared to baseline for the first 24 h (p ≤ 0.05 for both). Performance of 7 stimulation tests increased both basal and post-ACTH total and free cortisol concentrations from baseline by greater than 1.5 fold for the duration of the 96 h study (p ≤ 0.05). Neither SIM nor the performance of more stimulation tests affected delta cortisol measurements (total or free cortisol, p=NS). In contrast, dexamethasone suppressed basal total cortisol concentrations by greater than two-fold (p ≤ 0.005) at all time points and transiently increased delta total cortisol by approximately 35% during the first 24 h of the study (p ≤ 0.05). Conclusions total and free cortisol measurements—whether pre- or post-ACTH or as a calculated delta—were altered by intensive care therapies or frequent ACTH stimulation testing with one exception. Delta free cortisol was the only HPA measurement unaffected by SIM, completion of more ACTH stimulation tests or dexamethasone therapy. These findings support the need to determine normal ranges for HPA testing in subjects receiving ICM before establishing laboratory criteria for the diagnosis of RAI.

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“…Hormone analyses Plasma cortisol (Kemppainen, Thompson & Lorenz, 1983) and T4 levels were determined using radio¬ immunoassay kits (Diagnostic Products, Los Angeles, California, U.S.A.). Serial dilution and assay of a dog plasma pool gave inhibition curves with slopes similar (P>0-05) to the T4 standard.…”

Section: Introductionmentioning

confidence: 99%

Evidence for episodic but not circadian activity in plasma concentrations of adrenocorticotrophin, cortisol and thyroxine in dogs

Kemppainen¹,

Sartin²

1984

Journal of Endocrinology

134

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Concentrations of immunoreactive (i) ACTH, cortisol and thyroxine were determined in plasma samples obtained at 20-min intervals for 25 h in nine normal and two adrenalectomized dogs. The dogs were exposed to a 12 h light: 12 h darkness photoperiod for 30 days before the sampling period. Episodic secretion of iACTH and cortisol was evident in each normal dog, with an average of 9.0 iACTH peaks and 10.1 cortisol peaks in a 24-h period. Levels of iACTH and cortisol were significantly correlated in each normal dog, but periods of dissociation between levels of the two hormones were apparent. A sex difference in 24-h mean iACTH and cortisol levels, numbers of cortisol peaks, and amplitude of iACTH peaks was observed, with females showing higher mean levels and greater peak frequency and amplitude in each instance. Adrenalectomy resulted in a 50- to 150-fold increase in mean iACTH concentrations with an apparent increase in iACTH peak amplitude. Cortisol levels were unchanging in the adrenalectomized dogs. Thyroxine concentrations showed episodic variation in each of the normal dogs, but the mean number of peaks (3.3/24-h period) was considerably less than for iACTH or cortisol. Female dogs a had significantly higher 24-h mean levels of thyroxine than did males. No circadian rhythmicity was obvious for the plasma levels of any of the three hormones measured.

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Use of a low dose synthetic ACTH challenge test in normal and prednisone-treated dogs

Cited by 36 publications

References 4 publications

Effect of low doses of cosyntropin on serum cortisol concentrations in clinically normal dogs

Effect of low doses of cosyntropin on serum cortisol concentrations in clinically normal dogs

Defining normal adrenal function testing in the intensive care unit setting: A canine study*

Evidence for episodic but not circadian activity in plasma concentrations of adrenocorticotrophin, cortisol and thyroxine in dogs

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