Use of a Linear Accelerator for Conducting In Vitro Radiobiology Experiments

Hao, Jing; Magnelli, A.; Godley, A.; Yu, Jennifer

doi:10.3791/59514

Cited by 4 publications

(5 citation statements)

References 19 publications

(30 reference statements)

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Section: Radiation Treatmentmentioning

confidence: 99%

“…These groups received varying doses (2, 4, 6, 8, and 10 Gy) at a consistent dose rate of 3 Gy/min. The Eclipse treatment planning system (TPS) by Varian facilitated the precise assessment of the delivered doses [60,61]. Simultaneously, another set of caco-2 cells, treated and untreated with the cath L inhibitor, were not exposed to radiation and served as the negative control.…”

Section: Radiation Treatmentmentioning

confidence: 99%

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Enhancement of Radiation Sensitivity by Cathepsin L Suppression in Colon Carcinoma Cells

Abdelaziz,

Hussein,

Kotob

et al. 2023

IJMS

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Cancer is one of the main causes of death globally. Radiotherapy/Radiation therapy (RT) is one of the most common and effective cancer treatments. RT utilizes high-energy radiation to damage the DNA of cancer cells, leading to their death or impairing their proliferation. However, radiation resistance remains a significant challenge in cancer treatment, limiting its efficacy. Emerging evidence suggests that cathepsin L (cath L) contributes to radiation resistance through multiple mechanisms. In this study, we investigated the role of cath L, a member of the cysteine cathepsins (caths) in radiation sensitivity, and the potential reduction in radiation resistance by using the specific cath L inhibitor (Z-FY(tBu)DMK) or by knocking out cath L with CRISPR/Cas9 in colon carcinoma cells (caco-2). Cells were treated with different doses of radiation (2, 4, 6, 8, and 10), dose rate 3 Gy/min. In addition, the study conducted protein expression analysis by western blot and immunofluorescence assay, cytotoxicity MTT, and apoptosis assays. The results demonstrated that cath L was upregulated in response to radiation treatment, compared to non-irradiated cells. In addition, inhibiting or knocking out cath L led to increased radiosensitivity in contrast to the negative control group. This may indicate a reduced ability of cancer cells to recover from radiation-induced DNA damage, resulting in enhanced cell death. These findings highlight the possibility of targeting cath L as a therapeutic strategy to enhance the effectiveness of RT. Further studies are needed to elucidate the underlying molecular mechanisms and to assess the translational implications of cath L knockout in clinical settings. Ultimately, these findings may contribute to the development of novel treatment approaches for improving outcomes of RT in cancer patients.

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Section: Radiation Treatmentmentioning

confidence: 99%

Section: Radiation Treatmentmentioning

confidence: 99%

Enhancement of Radiation Sensitivity by Cathepsin L Suppression in Colon Carcinoma Cells

Abdelaziz,

Hussein,

Kotob

et al. 2023

IJMS

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“…Cells were irradiated with 0, 2, 4, 6, or 8 Gy using an Eleka Axesse medical linear accelerator (Elekta, Crawley, UK). A 1-cm bolus was placed on the top of the culture dish, and cells were irradiated with 6-MV photon beams at 600 MU/min [14].…”

Section: Cell Culture and Irradiationmentioning

confidence: 99%

miRNA-148a Enhances the Treatment Response of Patients with Rectal Cancer to Chemoradiation and Promotes Apoptosis by Directly Targeting c-Met

Huang

Chen

et al. 2021

Biomedicines

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Patients with locally advanced rectal cancer (LARC) who achieve a pathological complete response (pCR) to neoadjuvant chemoradiotherapy (NACRT) have an excellent prognosis, but only approximately 30% of patients achieve pCR. Therefore, identifying predictors of pCR is imperative. We employed a microRNA (miRNA) microarray to compare the miRNA profiles of patients with LARC who achieved pCR (pCR group, n = 5) with those who did not (non-pCR group, n = 5). The validation set confirmed that miRNA-148a was overexpressed in the pCR group (n = 11) compared with the non-pCR group (n = 40). Cell proliferation and clonogenic assays revealed that miRNA-148a overexpression radio-sensitized cancer cells and inhibited cellular proliferation, before and after irradiation (p < 0.01). Apoptosis assays demonstrated that miRNA-148a enhanced apoptosis before and after irradiation. Reporter assays revealed that c-Met was the direct target gene of miRNA-148a. An in vivo study indicated that miRNA-148a enhanced the irradiation-induced suppression of xenograft tumor growth (p < 0.01). miRNA-148a may be a biomarker of pCR following NACRT and can promote apoptosis and inhibit proliferation in CRC cells by directly targeting c-Met in vitro and enhancing tumor response to irradiation in vivo.

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“…A good target for producing X-rays comes from materials that have a high atomic number [1] . LINAC devices are widely used as radiotherapy equipment, especially for cancer or tumor therapy [2] .…”

Section: Introductionmentioning

confidence: 99%

Simulation Of Neutron Contamination In 6, 8, 10, 15, 18 and 25 MV Voltage Linac Device Using The Monte Carlo Method

Bilalodin

Haryadi²,

Haryanto³

2022

Indonesian J Appl Phys

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<p>A research on neutron contamination in LINAC device has been carried out using a Monte Carlo method. The simulation is based on the Siemens Primus LINAC machine model whose component consists of target, primary collimator, flattening filter and secondary collimator as its main components. A neutron contamination examination was carried out using a 10 x 10 cm radiation field and a 100 cm SSD. Subsequently, at a distance of 100 cm from the X-ray source, a water phantom is placed. Investigation of the presence of contaminants was carried out the LINAC operating voltages of 6, 8, 10, 15, 18 and 25 MV. The simulation results show that neutron contamination occurs due to the interaction of photons with the components of the LINAC device, namely the primary collimator, flattening filter and secondary collimator. The operating voltages that can produce neutron contaminant start at 10 MV. Increase in the voltage of the LINAC device causes consequent increase in neutron flux. Such increase in neutron flux has the potential to increase therapeutic dose.</p>

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Use of a Linear Accelerator for Conducting In Vitro Radiobiology Experiments

Cited by 4 publications

References 19 publications

Enhancement of Radiation Sensitivity by Cathepsin L Suppression in Colon Carcinoma Cells

Enhancement of Radiation Sensitivity by Cathepsin L Suppression in Colon Carcinoma Cells

miRNA-148a Enhances the Treatment Response of Patients with Rectal Cancer to Chemoradiation and Promotes Apoptosis by Directly Targeting c-Met

Simulation Of Neutron Contamination In 6, 8, 10, 15, 18 and 25 MV Voltage Linac Device Using The Monte Carlo Method

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