Use of a highly transparent zebrafish mutant for investigations in the development of the vertebrate auditory system
(Conference Presentation)

Wisniowiecki, Anna M.; Mattison, Scott P.; Kim, Sang-Min; Riley, Bruce B.; Applegate, Brian E.

doi:10.1117/12.2213087

Cited by 1 publication

(1 citation statement)

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“…Casper zebrafish were used throughout the study due to enhanced optical clarity and utility in fluorescent-based experimentation, without the need for phenylthiourea (PTU) treatment, which has been shown to influence both behaviour and neural crest development (Bohnsack, Gallina, & Kahana, 2011;Parker, Brock, Millington, & Brennan, 2013). Other groups have also used the casper mutant larvae to observe the zebrafish auditory system (Wisniowiecki, Mattison, Kim, Riley, & Applegate, 2016) and demonstrate similar ototoxicity responses in both casper mutants and wild-type larvae (d'Alençon et al, 2010). Embryos were collected and grown in E3 medium (5 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl 2 , 0.33 mM MgSO 4 ) in an incubator maintained at 28°C.…”

Section: Discussionmentioning

confidence: 99%

The identification of dual protective agents against cisplatin-induced oto- and nephrotoxicity using the zebrafish model

Wertman

Melong

Stoyek

et al. 2020

eLife

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Dose-limiting toxicities for cisplatin administration, including ototoxicity and nephrotoxicity, impact the clinical utility of this effective chemotherapy agent and lead to lifelong complications, particularly in pediatric cancer survivors. Using a two-pronged drug screen employing the zebrafish lateral line as an in vivo readout for ototoxicity and kidney cell-based nephrotoxicity assay, we screened 1280 compounds and identified 22 that were both oto- and nephroprotective. Of these, dopamine and L-mimosine, a plant-based amino acid active in the dopamine pathway, were further investigated. Dopamine and L-mimosine protected the hair cells in the zebrafish otic vesicle from cisplatin-induced damage and preserved zebrafish larval glomerular filtration. Importantly, these compounds did not abrogate the cytotoxic effects of cisplatin on human cancer cells. This study provides insights into the mechanisms underlying cisplatin-induced oto- and nephrotoxicity and compelling preclinical evidence for the potential utility of dopamine and L-mimosine in the safer administration of cisplatin.

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