“…Casper zebrafish were used throughout the study due to enhanced optical clarity and utility in fluorescent-based experimentation, without the need for phenylthiourea (PTU) treatment, which has been shown to influence both behaviour and neural crest development (Bohnsack, Gallina, & Kahana, 2011;Parker, Brock, Millington, & Brennan, 2013). Other groups have also used the casper mutant larvae to observe the zebrafish auditory system (Wisniowiecki, Mattison, Kim, Riley, & Applegate, 2016) and demonstrate similar ototoxicity responses in both casper mutants and wild-type larvae (d'Alençon et al, 2010). Embryos were collected and grown in E3 medium (5 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl 2 , 0.33 mM MgSO 4 ) in an incubator maintained at 28°C.…”