Use of a heterologous monoclonal antibody for cloning and detection of glial fibrillary acidic protein in the bovine ventricular ependyma

Bouchard, Philippe; Ravet, Viviane; Meiniel, R; Creveaux, Isabelle; Meiniel, Annie; Vellet, A.; Viguès, Bernard

doi:10.1007/s004419900077

Cited by 5 publications

(6 citation statements)

References 31 publications

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“…3). No GFAP immunopositivity was found in the SCO, in accordance with former data (Didier et al 1986;Ka´lma´n and Hajo´s 1989;Chouaf et al 1991;Bouchard et al 1999), therefore, these results are not shown here in figures.…”

Section: Adult Rat: Circumventricular Organssupporting

confidence: 79%

“…Since in several CVOs the presence or absence of GFAP immunopositivity has been published by our group (Hajo´s and Ka´lma´n 1989;Ka´lma´n and Hajo´s 1989;Ka´lma´n et al 1993Ka´lma´n et al , 1998, or by others (Møller et al 1978;D'Amelio et al 1985;Didier et al 1986;Redecker 1990;Chouaf et al 1991;Bouchard et al 1999), the GFAP results are shown in figures when they provide new information.…”

Section: Introductionmentioning

confidence: 99%

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Heterogeneous occurrence of aquaporin-4 in the ependyma and in the circumventricular organs in rat and chicken

2006

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Aquaporins are selective water channel proteins critical in volume homeostasis. In the CNS AQP4 predominates, localized mainly in the glia limitans, the perivascular endfeet and ependyma. The present immunofluorescent study reveals the distribution of aquaporin-4 in the circumventricular organs in rat and chicken brains. The ventricular ependyma (especially in the third one), the subfornical organ, the area postrema, the rat pineal body (in part), and the vascular organ of lamina terminalis were marked by intense immunopositivity. Several areas, however, proved to be immunonegative: the central canal, the subcommissural organ, the ependymal zone of the median eminence in rat but its whole thickness in chicken, the subtrochlear organ, and the paraventricular organ. The immunostaining of the lateral septal and subseptal organs were similar to their environment. Results on developing rats suggested that the aquaporin-4 immunonegativity is a secondary phenomenon. Surveying other structural and functional features, no clear explanation of the heterogeneous occurrence of aquaporin-4 was found. The absence of aquaporin-4 seems to correlate with some features of the "ependymal organs" (thickened, pseudostratified ependyma, presence of blood-brain barrier) and with the avoidance of GFAP. On the other hand, the organs rich in aquaporin-4 have features of the "hypendymal organs" (glial and vascular plexus but no blood-brain barrier). There are organs, however, which do not fit into either group completely, i.e. the lateral septal and subseptal organs. Presence of tight junctions coincides with the absence of aquaporin-4 in the ependyma of spinal cord, the subcommissural organ and the ependyma of median eminence.

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Section: Adult Rat: Circumventricular Organssupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Heterogeneous occurrence of aquaporin-4 in the ependyma and in the circumventricular organs in rat and chicken

2006

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“…Nonetheless, neuronal precursors are negative for this antibody and permits differentiating cells from both neural and glial lineages (Orduz et al, 2015). Its expression has also been observed in cultured embryonic cortical progenitors and in the adult mammalian V‐SVZ (Chaichana et al, ) as well as in the canine area postrema (Oliveira et al, ). Rabbit polyclonal anti‐glial fibrillary acidic protein (GFAP) (Dako Cat# Z0334 RRID:AB_10013382), an affinity‐purified antibody used for the identification of GFAP in astrocytic cells in the central nervous system that recognizes GFAP isolated from cow spinal cord and distinguishes astrocytes from other glial cells (Bouchard et al, ). It has been described that is expressed in the V‐SVZ of the adult brain mammalian species including rodents (Capilla‐González et al, 2014; Sohn et al, ) and dogs (Boya & Calvo, ; De Nevi et al, ). Rabbit polyclonal anti‐Laminin (Dako Cat# Z0097 RRID:AB_2313665) is an affinity‐purified antibody that recognizes laminins present in the basal membrane surrounding normal vessels.…”

Section: Methodsmentioning

confidence: 99%

Characterization of the canine rostral ventricular‐subventricular zone: Morphological, immunohistochemical, ultrastructural, and neurosphere assay studies

Fernández-Flores

García‐Verdugo

Martín‐Ibáñez

et al. 2017

J of Comparative Neurology

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The mammalian ventricular-subventricular zone (V-SVZ) presents the highest neurogenic potential in the brain of the adult individual. In rodents, it is mainly composed of chains of neuroblasts. In humans, it is organized in layers where neuroblasts do not form chains. The aim of this study is to describe the cytoarchitecture of canine V-SVZ (cV-SVZ), to assess its neurogenic potential, and to compare our results with those previously described in other species. We have studied by histology, immunohistochemistry (IHC), electron microscopy and neurosphere assay the morphology, cytoarchitecture and neurogenic potential of cV-SVZ. Age groups of animals were performed. Histological and ultrastructural studies indicated that the cV-SVZ is organized in layers as in humans, but including migratory chains as in rodents. Neural progenitors were organized in niches in the subependymal area and a decline in their number was observed with age. Adult-young dogs contained migratory cells capable to expand and differentiate in vitro according with previous results obtained in rodents, primates, humans, pigs, and dogs. Some adult animals presented perivascular niches outside the V-SVZ. Our observations evidence a great similarity between canine and human V-SVZ indicating that the dog may be better representative of neurogenic events in humans, compared with rodents. Accordingly with our results, we conclude that dogs are a valuable animal model of adult neurogenesis in comparative and preclinical studies.

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“…We further identified the receptor subtypes responsible for SP-and ATP-activated calcium signaling. Our data give specific insight into intracellular processes that may serve the regulation of SCO activity, and they provide general information on neuron-glia interactions, since SCO cells are thought to belong to the ependymal/astroglial cell lineage (Bouchard et al 1999). …”

Section: Introductionmentioning

confidence: 96%

Effects of neuroactive substances on the activity of subcommissural organ cells in dispersed cell and explant cultures

Schöniger

Kopp

Schomerus

et al. 2002

Cell and Tissue Research

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The subcommissural organ (SCO), an ependymal (glial) circumventricular organ, releases glycoproteins into the cerebrospinal fluid; however, the regulation of its secretory activity is largely unknown. To identify neuroactive substances that may regulate SCO activity, we investigated immunocytochemically identified bovine SCO cells by means of calcium imaging. This analysis was focused on: (1) serotonin (5HT) and substance P (SP), immunocytochemically shown to be present in axons innervating the bovine SCO; and (2) ATP, known to activate glial cells. 5HT had no effect on the intracellular calcium concentration ([Ca(2+)](i)), and its precise role remains to be clarified. SP elicited rises in [Ca(2+)](i) in approx. 30% and ATP in even 85% of the analyzed SCO cells. These effects were dose-dependent, involved NK(3) and P2Y(2) receptors linked to G protein and phospholipase C (PLC) activation, and could not be mimicked by forskolin or 8-bromo-cAMP. In 50% of the SP-sensitive cells, the increases in [Ca(2+)](i) comprised calcium release from thapsigargin-sensitive intracellular stores and an influx of extracellular calcium via protein kinase C (PKC)-induced opening of L-type voltage-gated calcium channels (VGCCs). In the remaining SP-sensitive cells, the increase in [Ca(2+)](i) was caused exclusively by influx of extracellular calcium via VGCCs of the L-type. In all ATP-sensitive cells the increase in [Ca(2+)](i) involved calcium release from thapsigargin-sensitive intracellular stores and a PKC-mediated influx of extracellular calcium via L-type VGCCs. Our data suggest that SP and ATP are involved in regulation of the activity of SCO cells.

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Use of a heterologous monoclonal antibody for cloning and detection of glial fibrillary acidic protein in the bovine ventricular ependyma

Cited by 5 publications

References 31 publications

Heterogeneous occurrence of aquaporin-4 in the ependyma and in the circumventricular organs in rat and chicken

Heterogeneous occurrence of aquaporin-4 in the ependyma and in the circumventricular organs in rat and chicken

Characterization of the canine rostral ventricular‐subventricular zone: Morphological, immunohistochemical, ultrastructural, and neurosphere assay studies

Effects of neuroactive substances on the activity of subcommissural organ cells in dispersed cell and explant cultures

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