Use of a Fibrin Preparation in the Engineering of a Vascular Graft Model

Aper, Thomas; Teebken, Omke E.; Steinhoff, Gustav; Haverich, Axel

doi:10.1016/j.ejvs.2004.05.016

Cited by 51 publications

(29 citation statements)

References 16 publications

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“…In fact, Aper et al found that ECs adhered to fibrin gels extremely well and formed near confluent monolayers that remained viable for up to 19 days. 3 In either case, more work needs to be done to more precisely determine the composition of the luminal surface and the origin of the enhanced adhesion, which may have implications for improving EC retention on traditional synthetic grafts as well as tissue-engineered grafts.…”

Section: Discussionmentioning

confidence: 99%

Endothelialization and Flow Conditioning of Fibrin-Based Media-Equivalents

2006

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It is generally accepted that endothelialization and subsequent development of a functional endothelium are of paramount importance to the success of any bioartificial artery. In this study, we aimed to assess the ability of smooth muscle cell-remodeled, fibrin-based media-equivalents (MEs) to be endothelialized, examine the morphological changes of endothelial cells (ECs) associated with exposure to physiologically-relevant shear stress in a custom-built bioreactor, and determine if adherent ECs are capable of withstanding average physiological shear stresses. It was found that MEs could be readily endothelialized with surface coverages of 98.8 +/- 0.9% after two days, and the ECs expressed von Willebrand factor. Furthermore, EC retention remained high (steady: 96.5 +/- 4.4%, pulsatile: 94.3 +/- 4.3%) under exposure to physiologically relevant shear stresses for 48 h. The results indicate that these MEs are conducive to generating an EC monolayer, with the ECs possessing adhesion strength sufficient to withstand physiological shear stress and maintain a normal phenotype.

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Section: Discussionmentioning

confidence: 99%

Endothelialization and Flow Conditioning of Fibrin-Based Media-Equivalents

2006

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“…The unique potential of fibrin as a scaffold has been well documented. 2,9,13,19,23,33,37 The necessity to optimize the concentrations of fibrin scaffold components 7,14 and fibrinolytic inhibitors 15 has been highlighted previously. As a result, the seeding technique and the fibrin component concentrations used in this study were based on those previously optimized for tissue engineering vascular grafts.…”

Section: Introductionmentioning

confidence: 97%

Behavior of Human Mesenchymal Stem Cells in Fibrin-Based Vascular Tissue Engineering Constructs

et al. 2010

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A limitation of current tissue engineering vascular graft technology is the provision of an expandable, autologous cell source. By harnessing the multipotency of mesenchymal stem cells (MSC), it is hoped that functional vascular cells can be produced. To date, a range of 2D and 3D environments have been investigated for the manipulation of MSC differentiation pathways. To this end, this study aims to test the hypothesis that MSC seeded in various fibrin gel environments will exhibit evidence of a smooth muscle cell (SMC) phenotype. Initially, a range of cell-seeding densities were screened for 2D and 3D fibrin constructs, where it was observed that a seeding densities of 500,000 cells/mL facilitated gel compaction without degradation or loss in cell viability. Additionally, positive expression of CD49, CD73, CD105 markers and negative expression of hemopoietic stem cell-associated CD34 and CD45 indicated that MSC phenotype was retained within the fibrin gel. Nonetheless, a decrease in the gene expression of alpha-smooth cell actin and calponin was observed for MSC cultured in static 3D fibrin gels. Although a slight recovery was observed after 24 h mechanical stimulation, the fold-change remained significantly lower than that observed for cells cultured on 2D tissue culture plastic. While MSC differentiation toward a SMC appears possible in both 2D and 3D environments, scaffold architecture and mechanical stimulation undoubtedly play an important role in the creation of a functional SMC phenotype.

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“…Gerade Fibrin besitzt eine Reihe von Eigenschaften, die vorteilhaft sind für seine Verwendung als Trägerstruk-tur. So unterstützt es die Anhaftung und die Proliferation verschiedener Zelltypen und deren 3D-Anordnung in der Matrix [7]. Im Gegensatz zu Kollagen stimuliert es in angesiedelten Zellen die Synthese von Kollagen, Elastin und anderen Matrixproteinen mit entsprechender …”

Section: Biologische Matricesunclassified

Custom-made autologous bioartificial vascular grafts

Aper

2015

Z Herz- Thorax- Gefäßchir

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Use of a Fibrin Preparation in the Engineering of a Vascular Graft Model

Abstract: A structured coculture of MFB and EC was obtained mimicking the formation of a vascular wall with preserved viability utilizing a fibrin preparation. Nutrition problems seem to limit the maximal extent of MFB in the matrix.

Cited by 51 publications

References 16 publications

Endothelialization and Flow Conditioning of Fibrin-Based Media-Equivalents

Endothelialization and Flow Conditioning of Fibrin-Based Media-Equivalents

Behavior of Human Mesenchymal Stem Cells in Fibrin-Based Vascular Tissue Engineering Constructs

Custom-made autologous bioartificial vascular grafts

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