Use of a cyanine dye as a probe for albumin and collagen in the extracellular matrix

Панова, И. Г.; Шарова, Н. П.; Dmitrieva, S. B.; Полтавцева, Р. А.; Сухих, Г. Т.; Татиколов, А. С.

doi:10.1016/j.ab.2006.11.029

Cited by 29 publications

(18 citation statements)

References 13 publications

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“…Indeed, a number of meso-substituted cyanine dyes have already been used as spectral-fluorescent probes for biomacromolecules (DNA, albumins, etc.) [71][72][73][74][75][76][77]. Next in turn is the use of polymethine dyes as kinetic probes, as it has been found recently that the lifetime of the photoisomers of meso-substituted oxonols (anionic polymethine dyes) [78] and squarylium cyanines [79] depend strongly on the polarity of the molecular microenvironment.…”

Section: Discussionmentioning

confidence: 99%

Isomerization and Properties of Isomers of Carbocyanine Dyes

Пронкин

Татиколов

2018

Sci

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One of the important features of polymethine (cyanine) dyes is isomerization around one of C–C bonds of the polymethine chain. In this review, spectral properties of the isomers, photoisomerization and thermal back isomerization of carbocyanine dyes, mostly meso-substituted carbocyanine dyes, are considered. meso-Alkyl-substituted thiacarbocyanine dyes are present in polar solvents mainly as cis isomers and, hence, exhibit no photoisomerization, whereas in nonpolar solvents, in which the dyes are in the trans form, photoisomerization takes place. In contrast, the meso-substituted dyes 3,3′-dimethyl-9-phenylthiacarbocyanine and 3,3′-diethyl-9-(2-hydroxy-4-methoxyphenyl)thiacarbocyanine occur as trans isomers and exhibit photoisomerization in both polar and nonpolar solvents. The behavior of these dyes may be explained by the fact that the phenyl ring of the substituent in their molecules can be twisted at some angle, removing the substituent from the plane of the molecule and reducing its steric effect on the conformation of the trans isomer. In some cases, photoisomerization of cis isomers of meso-substituted carbocyanine dyes is also observed (for some meso-alkyl-substituted dyes complexed with DNA and chondroitin-4-sulfate; for 3,3′-diethyl-9-methoxythiacarbocyanine in moderate polarity solvents). The cycle photoisomerization–thermal back isomerization of cyanine dyes can be used in various systems of information storage and deserves further investigation using modern research methods.

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Section: Discussionmentioning

confidence: 99%

Isomerization and Properties of Isomers of Carbocyanine Dyes

Пронкин

Татиколов

2018

Sci

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“…Calibration curve was plotted by adding albumin in certain concentrations to the dye solution. The intensity of absorption band with the maximum at λ=612 corresponding to trans-form of the stain forming in albumin reaction with the stain was measured [1,10,12]. The absorption spectra were measured in a 1-cm cuvette on a UV-3101PC spectrophotometer (Shimadzu) at λ=400-800 nm.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, it was shown that human vitreous body contained sufficiently high amounts of albumin during prenatal development [1,10]. Albumin is known as a carotenoid carrier protein [11], which attests to a correlations between carotenoids and albumin levels in human fetal vitreous body.…”

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confidence: 99%

Correlation between the content of albumin and carotenoids in human vitreous body during prenatal development

2007

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The dynamics of the content albumin and carotenoids in human fetal vitreous body during weeks 16-31 of gestation was studied. The maximum values of total albumin (1.42 mg) and carotenoids (276 ng) during the studied period were recorded on weeks 20-22. Albumin concentration peaked during week 17 (2.11x10(-4) mol/liter) and carotenoids during weeks 16-17 (about 0.045x10(-4) mol/liter) of prenatal development. By week 31, the concentrations and total content of albumin and carotenoids in the vitreous body decreased. The physiological role of the studied components of the vitreous body for prenatal development of human eye is discussed.

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“…Due to their photophysic properties, this class of compounds presents numerous applications, like photographic sensitizers [3,4], infrared-dyesensitized solar cell [5], optical recording [6], nonlinear frequency doublers [7,8], or biological probes [9][10][11][12]. Cyanines are also known to possess interesting pharmacological properties [13,14].…”

Section: Introductionmentioning

confidence: 99%

Comparison of diffusivities data of streptocyanine dyes by electrochemical and NMR-DOSY methods

André-Barrès

Najjar

Maether

et al. 2012

Journal of Electroanalytical Chemistry

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a b s t r a c tDiffusivity data of streptocyanine dyes in acetonitrile were investigated using both transient voltammetric techniques and NMR-DOSY. Results of voltammetric measurements allow determination of the diffusion coefficient D values in the range of 1.2-1.6 Â 10 À9 m 2 s À1 for the target dyes. Deuterated acetonitrile solvent was used for NMR-DOSY experiments and diffusivities of streptocyanines are given after calibration of acetonitrile on its tracer diffusion value of D Ã ACN of 4.37 Â 10 À9 . The results for the two methods appear to be in good agreement allowing use of the convenient NMR-DOSY method to measure diffusivity of streptocyanine dyes at low concentrations and so giving a direct access to the number of exchanged electrons using limiting current electrochemical techniques. This last method affords a great advantage of obtaining diffusion coefficients by non-electrochemical method.

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Use of a cyanine dye as a probe for albumin and collagen in the extracellular matrix

Cited by 29 publications

References 13 publications

Isomerization and Properties of Isomers of Carbocyanine Dyes

Isomerization and Properties of Isomers of Carbocyanine Dyes

Correlation between the content of albumin and carotenoids in human vitreous body during prenatal development

Comparison of diffusivities data of streptocyanine dyes by electrochemical and NMR-DOSY methods

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