Use of a Centrifugal Analyzer in Coagulation Testing

Hills, Lorin P.; Lorenzi-Anderson, M.; Huey, Edith E.; Tiffany, T. O.

doi:10.1055/s-2007-1005023

Cited by 10 publications

(5 citation statements)

References 17 publications

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“…13 The use of equipment, such as the Multistat III, entails the possibility for profiling, that is performing a number of different clotting assays and factor analyses in one run. Screening tests, such as partial thromboplastin times, prothrombin times, and thrombin times can be run concurrently, 19 and Factor X assays, fibrinogen levels, antithrombin and heparin levels, plasminogen and α 2 -antiplasmin levels (and others) can be determined, 18 and fibrino gen split products as well as D-dimers should be available soon for these instruments. Thus, one can foresee a post-CPB bleeding diagnosis panel con sisting of a variety of key analyses, including a quantitative heparin assay.…”

Section: Discussionmentioning

confidence: 99%

“…Fibrinogen was determined manually by the method of Clauss 5 using the fibrinogen Data-Fi system (American Dade, Miami, FL) and a BBL Fibrometer (Cockeysville, MD). Fibrinogen was also measured by an automated turbidometric procedure 18 using a Multistat III F/LS centrifugal analyzer (Instrumentation Laboratory, Lexington, MA). The Multistat III is equipped with an 8K RAM programmable microcomputer using a "clot" tape that was generously supplied by Instrumentation Laboratory (Spokane, WA).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Hemostasis Changes During Cardiopulmonary Bypass Surgery

Mammen¹,

Koets²,

Washington³

et al. 1985

Semin Thromb Hemost

305

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A number of hemostasis parameters were studied in a total of 63 patients undergoing cardiopulmonary bypass (CPB) for open heart surgery. In 33 patients fibrinogen, Factors II, V, VIII:C, X, XI, antithrombin, plasminogen, alpha 2-antiplasmin, and platelet counts were assayed before surgery, during maximal hypothermia, at the end of the bypass procedure, before and after protamine sulfate infusion, in the intensive care unit, and 48 hours postoperatively. All factors assayed decreased markedly when the patients were placed on the bypass machine, the drop fairly well paralleling the decrease in hematocrit. During bypass the factors remained low, although a slight tendency toward an increase was noted. Only platelet counts remained low with a decreasing trend until the end of bypass. In the intensive care unit a second decrease in fibrinogen, Factors II and V and antithrombin was noted. This drop was unrelated to four patients who experienced a greater blood loss during this time than the others. Forty-eight hours postoperatively, a marked increase could be found in all clotting factors and near normal levels were measured. Platelet counts remained low, however. The decrease in factors rarely dropped into a range where one would expect a compromised hemostasis (less than 30%). Although antithrombin levels decreased below 60%, no difficulties with heparinization were encountered. Several factors were assayed manually and by automated analyzer (Multistat III), and excellent correlations were found between both procedures. Also a good correlation was found between the activated whole blood clotting times and quantitative heparin assays. In 30 additional patients platelet function was studied before surgery, after thoracotomy, after heparin administration, after initiation of bypass, at maximal hypothermia, before and after protamine sulfate infusion, and 24 hours postoperatively. Platelet counts once again decreased as patients were placed on the CPB machine and remained low throughout the procedure. Mean platelet volumes were unchanged until protamine was given. At that time, a significant drop in mean platelet volume was recorded. Twenty-four hours postoperatively the volumes were normal again. Platelet aggregation studies were performed on a whole blood aggregometer using two concentrations of ADP, collagen, and ristocetin as aggregation inducers. A significant decrease in aggregability was seen when the patients were connected to the CPB apparatus.(ABSTRACT TRUNCATED AT 400 WORDS)

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Hemostasis Changes During Cardiopulmonary Bypass Surgery

Mammen¹,

Koets²,

Washington³

et al. 1985

Semin Thromb Hemost

305

View full text Add to dashboard Cite

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“…Functional and immunochemical assays are essential and have been constantly updated for most of these proteins and proteases. 1,7,19,23,34 Synthetic substrate methods that are automatable are of great interest, particularly for Factor VIII, Factor IX, and the vitamin K dependent factors (anticoagulant control). A suitable Factor VIII method has been recently developed.…”

Section: Assays Of Enzymes Of the Coagulation Cascadementioning

confidence: 99%

Automation in Coagulation Testing Clinical Applications

Messmore¹

1983

Semin Thromb Hemost

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“…Ecosystem services (ESs) make an important contribution to human well-being, directly or indirectly [1]. The Millennium Ecosystem Services Assessment [2] divided ESs into four categories: supporting services, provision services, regulation services, and cultural services. This means that ecosystems can not only provide people with food and production materials and regulate the climate and carbon cycle, but can also provide landscape and entertainment services that meet the needs of human spiritual life [2,3].…”

Section: Introductionmentioning

confidence: 99%

“…The Millennium Ecosystem Services Assessment [2] divided ESs into four categories: supporting services, provision services, regulation services, and cultural services. This means that ecosystems can not only provide people with food and production materials and regulate the climate and carbon cycle, but can also provide landscape and entertainment services that meet the needs of human spiritual life [2,3]. Different types of ESs have complex interactions, and trade-offs and synergies are two primary relations among them [4].…”

Section: Introductionmentioning

confidence: 99%

Trade-Offs and Synergies of Ecosystem Services in the Pearl River Delta Urban Agglomeration

et al. 2021

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Since ecosystem services (ESs) have become effective tools for urban planning, spatiotemporal analysis of regional ESs and a deep understanding of the trade-offs among ESs are of great significance to regional governance. In this study, the spatial and temporal changes of four basic ESs were analyzed by combining statistical data with the InVEST model across the Pearl River Delta (PRD) urban agglomeration, China. The trade-offs among the related ESs were analyzed at the urban agglomeration scale and the city scale by correlation analysis. The results showed that: (1) Construction land increased by 6.78% from 2000 to 2018, while cultivated land and forest decreased. (2) Water yield showed an increasing trend, while carbon storage, food production, and habitat quality showed a downward trend from 2000 to 2018. (3) The four ecosystem services were significantly correlated, with synergies existing between water yield and food production, and between habitat quality and carbon storage, while other relationships are trade-offs. What is more, the scale has little influence on the direction of ES trade-off or synergy but influences the degree of the relationship. This empirical evidence on ES relationships in urban agglomerations can provide a reference for the sustainable development of ESs and efficient management of urban agglomerations.

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Use of a Centrifugal Analyzer in Coagulation Testing

Cited by 10 publications

References 17 publications

Hemostasis Changes During Cardiopulmonary Bypass Surgery

Hemostasis Changes During Cardiopulmonary Bypass Surgery

Automation in Coagulation Testing Clinical Applications

Trade-Offs and Synergies of Ecosystem Services in the Pearl River Delta Urban Agglomeration

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