Use of a bacterial expression vector to map the varicella-zoster virus major glycoprotein gene, gC

Ellis, Ronald W.; Keller, Paul M.; Lowe, Robert S.; Zivin, Robert A.

doi:10.1128/jvi.53.1.81-88.1985

Cited by 47 publications

(11 citation statements)

References 27 publications

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“…Finally, no homology between EHV-1 and EHV-3 U. sequences could be detected under the stringent hybridization conditions used in these studies. Why this region alone should be less conserved than other genomic areas is uncertain, but studies on pseudorabies virus (17,27) and varicellazoster virus (10), which are herpesviruses with a two-isomer type of structure similar to that of EHV-1 and EHV-3, have indicated that coding sequences for important viral glycoproteins are localized within the U, regions of these two viruses. Perhaps the EHV-1 and EHV-3 Us regions encode protein products (such as surface glycoproteins) which im- part some of the differential properties, such as serological type specificity, which serve to distinguish these two viruses.…”

Section: Discussionmentioning

confidence: 99%

Genetic relatedness and colinearity of genomes of equine herpesvirus types 1 and 3

Baumann¹,

Sullivan²,

Staczek³

et al. 1986

J Virol

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The arrangement and location of homologous DNA sequences within the genomes of equine herpesvirus type 1 (EHV-1) and EHV-3 were investigated by using Southern blot hybridization analyses conducted under stringent conditions. Recombinant plasmid libraries comprising 95 and 84% of the EHV-1 and EHV-3 genomes, respectively, were labeled with 32P-deoxynucleotides by nick translation and were used as probes in filter hybridization studies. The DNA homology between the EHV-1 and EHV-3 genomes was dispersed throughout the genomes in a colinear arrangement. Significant hybridization was detected between the EHV-1 short region inverted repeat sequences, which are known to encode immediate early transcripts, and the corresponding EHV-3 inverted repeat sequences. Interestingly, probes derived from the EHV-1 heterogeneous region, which is adjacent to the EHV-1 short region, hybridized strongly to EHV-3 DNA sequences within a similar genomic location, but did not reveal any corresponding heterogeneity within the EHV-3 genome. Our results demonstrated that there is a highly conserved evolutionary relationship between EHV-1 and EHV-3 and provided the foundation for further investigations to determine whether similarities in protein function underpin the genetic relatedness between these two herpesviruses.

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Section: Discussionmentioning

confidence: 99%

Genetic relatedness and colinearity of genomes of equine herpesvirus types 1 and 3

Baumann¹,

Sullivan²,

Staczek³

et al. 1986

J Virol

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“…The VZV glycoprotein gI (previously designated gpIV) and gE are encoded by VZV genes 67 and 68, respectively, which are located within the SalI-I DNA fragment ( Fig. 1) of the unique short sequences (Us) of the VZV genome [14,28,29].…”

Section: Vzv Ge (Gpi)mentioning

confidence: 99%

Varicella-Zoster Virus Subunit Vaccine

Vafai¹

2009

TOVACJ

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Varicella-zoster virus (VZV) causes chickenpox (Varicella) in children and shingles (Zoster) in elderly and immunosuppressed individuals. An attenuated VZV vaccine has been approved for general immunization to prevent varicella and zoster in the United States. Although this vaccine provides a high degree of protection against virus infection, the virus becomes latent in dorsal root ganglia and will reactivate to produce zoster. Therefore, there is a need for developing additional VZV vaccines that are capable of eliciting immune response to the virus but will not establish viral latency. Our studies have been focused on the development of a truncated secretory VZV glycoprotein (VZVgE) subunit vaccine. The results from our studies have shown that the VZV subunit vaccine: (1) elicits the induction of neutralizing antibodies in animals as well as in humans; and (2) stimulates the induction of VZVgE-speci¿ c antibodies in VZV-seropositive human mononuclear cells. Such a VZV glycoprotein antigen, therefore, may have the potential to be used as a candidate VZV glycoprotein subunit vaccine for prevention of primary VZV infection (Varicella) or boosting immune response against VZV reactivation (Zoster) in adults, the elderly and immunosuppressed individuals.

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“…The same region of gI is homologous to a region of the VZV protein designated 70K in the original sequencing report (9). In the new nomenclature system this protein is designated gpl (10) and is identified as one of the VZV glycoproteins that has been characterized by monoclonal antibodies (32) and expression in E. coli (13). By insertion of minor gaps in the alignment, six cysteine residues can be aligned, indicating the conservation of what are likely some important disulfide bonds for the structures of these glycoproteins.…”

Section: Gcgatccggagaaaccggaagtgacgaatgggcccaactatggcgtgaccgccagccgccmentioning

confidence: 99%

Use of lambda gt11 to isolate genes for two pseudorabies virus glycoproteins with homology to herpes simplex virus and varicella-zoster virus glycoproteins

Petrovskis¹,

Timmins²,

Post³

1986

J Virol

108

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A library of pseudorabies virus (PRV) DNA fragments was constructed in the expression cloning vector Agtll. The library was screened with antisera which reacted with mixtures of PRV proteins to isolate recombinant bacteriophages expressing PRV proteins. By the nature of the Agtll vector, the cloned proteins were expressed in Escherichia coli as I-galactosidase fusion proteins. The fusion proteins from 35 of these phages were purified and injected into mice to raise antisera. The antisera were screened by several different assays, including immunoprecipitation of [14C]glucosamine-labeled PRV proteins. This method identified phages expressing three different PRV glycoproteins: the secreted glycoprotein, gX; gI; and a glycoprotein that had not been previously identified, which we designate gp63. The gp63 and gI genes map adjacent to each other in the small unique region of the PRV genome. The DNA sequence was determined for the region of the genome encoding gp63 and gI. It was found that gp63 has a region of homology with a herpes simplex virus type 1 (HSV-1) protein, encoded by US7, and also with varicella-zoster virus (VZV) gpIV. The gI protein sequence has a region of homology with HSV-1 gE and VZV gpl. It is concluded that PRV, HSV, and VZV all have a cluster of homologous glycoprotein genes in the small unique components of their genomes and that the organization of these genes is conserved. * Corresponding author. another major glycoprotein, glil, has been mapped (40) and shown to have homology with the HSV gC gene (39). The gene for a minor PRV glycoprotein, gp5O, was mapped by

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Use of a bacterial expression vector to map the varicella-zoster virus major glycoprotein gene, gC

Cited by 47 publications

References 27 publications

Genetic relatedness and colinearity of genomes of equine herpesvirus types 1 and 3

Genetic relatedness and colinearity of genomes of equine herpesvirus types 1 and 3

Varicella-Zoster Virus Subunit Vaccine

Use of lambda gt11 to isolate genes for two pseudorabies virus glycoproteins with homology to herpes simplex virus and varicella-zoster virus glycoproteins

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