Use molecular technique to detect toxoplasmosis in placenta and aborted fetus in women in Al-Diwaniyah province

Abstract

The current study included the detection of Toxoplasma gondii from aborted women from Maternity and Childhood Teaching Hospital. It drew attention to the human infection rate in order to detect the differentiation them with SAG3 gene. The samples were collected during the period from October, 2021 until the end of March 2022 in AL-Qadisyah province. Total of 100 samples (placenta and fetus) were collected and send to the laboratory of veterinary medicine AL- Qadisiyah University in order to detect women with toxoplasmosis by impression method all of are positive. The present study included a molecular method to detect surface antigen 3 (SAG3) using Nested Polymerase Chain Reaction (nPCR), the results of the nPCR for SAG3 gene showed that 18/24 (75 %) were positive in the aborted woman. the NCBI-BLAST Homology Sequence identity (%) between local T. gondii isolated from women cases that have been deposited in the NCBI under the following accession numbers (ON240954, ON240955, ON240956, ON240957, ON240958, ON240959, ON240960, ON240961, ON240962, and ON240963) and NCBI-BLAST deposited other global strains.