Use and interpretation of virological tests for hepatitis C

Pawlotsky, Jean‐Michel

doi:10.1053/jhep.2002.36815

Cited by 150 publications

(86 citation statements)

References 60 publications

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“…Currently in the United States, HCV testing is recommended at least once for persons born between 1945 and 1965 [6] . A positive test result for anti-HCV antibody indicates either current infection -acute or chronic, previous infection that may have resolved, or a false positive test result [16] . For individuals with a positive test result, confirmatory test (HCV RNA) to confirm viremia should be performed.…”

Section: Chronic Hcv Infectionmentioning

confidence: 99%

Hepatitis C cirrhosis: New perspectives for diagnosis and treatment

Khullar

Firpi

2015

WJH

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Chronic hepatitis C infection is the leading cause of chronic liver disease, cirrhosis, hepatocellular carcinoma as well as the primary indication for liver transplantation in the United States. Despite recent advances in drugs for the treatment of hepatitis C, predictive models estimate the incidence of cirrhosis due to hepatitis C infection will to continue to rise for the next two decades. There is currently an immense interest in the treatment of patients with fibrosis and early-stage cirrhosis as treatment can lead to decrease in the rates of decompensated cirrhosis, hepatocellular carcinoma and need for liver transplantation in these patients. The goal of this paper is to provide clinicians and health care professionals further information about the treatment of patients with hepatitis C infection and cirrhosis. Additionally, the paper focuses on the disease burden, epidemiology, diagnosis and the disease course from infection to treatment. We provide an overview of multiple studies for the treatment of chronic hepatitis C infection that have included patients with cirrhosis. We also discuss the advantages and disadvantages of treatment in cirrhotic patients and focus on the most up to date guidelines available for treatment.

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Section: Chronic Hcv Infectionmentioning

confidence: 99%

Hepatitis C cirrhosis: New perspectives for diagnosis and treatment

Khullar

Firpi

2015

WJH

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“…Virological diagnosis and monitoring of (HCV) are based on two categories of laboratory tests: indirect tests, namely serologic assays which detecting specific antibody to HCV (anti-HCV) and direct tests, namely molecular assays which detect, quantify, or characterize components of the viral particle, such as HCV RNA testing and HCV core antigen detection testing.Direct and indirect virological tests play a key role in the diagnosis of infection, therapeutic decision-making, and assessment of the virological response to therapy (Pawlotsky, 2002). …”

Section: Introduction:-mentioning

confidence: 99%

“…Antigen-antibody complexes are then specifically revealed in a colorimetric enzymatic reaction. After reading in a spectrophotometer, the result is expressed as the ratio of the optical density of the test sample to that of a kit control (Pawlotsky, 2002).…”

Section: Introduction:-mentioning

confidence: 99%

Hepatitis C virus infection and its impact on Liver function in chronic HCV patients.

Suef¹,

Mohamed²,

Farag³

et al. 2016

IJAR

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Hepatitis C virus (HCV) is a blood-borne virus whose target organ is the liver. It causes both acute and chronic hepatitis, acute infection becomes chronic in approximately 80% of cases, and patients with chronic hepatitis C areat high risk of life-threatening complications, including cirrhosis in 20% of cases and hepatocellular carcinoma at an incidenceof 4-5% per year in cirrhotic patients. Virological testing has become essential in the management of HCV infection in order to diagnose infection, and most importantly guide treatment decisions and assess the virological response to antiviral therapy. Thus, the aim of presenting work was to investigate the relationship between the viral load and Liver biochemical/function testing according to the results of the laboratory tests.To achieve that aim, 20 chronic hepatitis C patients, enrolled from National Institute of Liver Disease Shebeen EL Koom Menofyia Governorate, Egypt. Blood samples were collected for different analysis including serological test by ELISA and molecular assays by qualitative polymerase chain reaction (PCR), also a routine lab test for measuring liver function test has been done. All patients included in our study were positive for anti-HCV antibody by ELISA test and were positive for the presence of HCV-RNA which detected by PCR. In conclusion, there is no significant variance between liver enzymes function and viral load. Data analysis for detecting HCV was an important factor for understanding the epidemiology and treatment strategies of HCV among Egyptian patients.Copy Right, IJAR, 2016,. All rights reserved.

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“…Although many studies of the immunological mechanisms involved in patient prognosis have been conducted during the past 2 decades, no suitable immunological monitoring system to predict the prognosis of each patient is available at the present time [1,6,7,13,14,16,18,19]. In acute resolving hepatitis, HCV RNA is cleared in some individuals, whereas the majority (>50%) do not clear the virus, but instead develop chronic hepatitis (CH).…”

Section: Introductionmentioning

confidence: 99%

“…This lack of useful Wndings might in part be due to an insuYcient understanding of the biological roles of antibodies (Abs) that are speciWc to HCV. There are several lines of evidence suggesting the involvement of cytotoxic T-lymphocyte (CTL) and T helper cell responses in viral eradication [1,6,7,13,14,16,18,19]. However, it is diYcult to carry out CTL and T helper cell assays, which in any case are not sensitive enough to be utilized as laboratory markers.…”

Section: Introductionmentioning

confidence: 99%

Serum levels of IgG to the peptide of HCV1b core at positions 35–44 correlated with persistent infection, while levels of IgG to the peptide of NS5A at positions 2132–2140 correlated with better prognosis in HCV-infected patients

Takao

Yamada

Yutani

et al. 2007

Med Microbiol Immunol

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We previously reported that two IgG Abs to the hepatitis C virus (HCV), anti-core 35-44 (C35) and anti-NS5A 2132-2140 (NS5A2132), existed in the sera of the majority of patients with HCV infection. This study investigated if measuring the two Abs would facilitate the prediction of a patient's prognosis. The serum levels of anti-C35 were found to correlate with persistent infection, while those of anti-NS5A2132 correlated with a better prognosis in HCV-infected patients. These results suggest that sequential measurement of the two Abs together may provide new information for the prediction of prognosis.

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Use and interpretation of virological tests for hepatitis C

Cited by 150 publications

References 60 publications

Hepatitis C cirrhosis: New perspectives for diagnosis and treatment

Hepatitis C cirrhosis: New perspectives for diagnosis and treatment

Hepatitis C virus infection and its impact on Liver function in chronic HCV patients.

Serum levels of IgG to the peptide of HCV1b core at positions 35–44 correlated with persistent infection, while levels of IgG to the peptide of NS5A at positions 2132–2140 correlated with better prognosis in HCV-infected patients

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