Usage of Bacterial Artificial Chromosomes for Studying BDNF Gene Regulation in Primary Cultures of Cortical Neurons and Astrocytes

Jaanson, Kaur; Pärn, Angela; Timmusk, Tõnis

doi:10.1007/7657_2018_10

Cited by 2 publications

(1 citation statement)

References 38 publications

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“…Cultured cortical neurons were transfected as described previously ( Jaanson et al, 2019 ) with minor modifications. Transfection was carried out in unsupplemented NBA using 500 ng of the luciferase reporter construct and 20 ng of a normalizer plasmid pGL4.83-mPGK-hRLuc at 5–6 DIV using Lipofectamine 2000 (Thermo Scientific) with DNA to Lipofectamine ratio of 1:2.…”

Section: Methodsmentioning

confidence: 99%

Intronic enhancer region governs transcript-specific Bdnf expression in rodent neurons

Tuvikene

Esvald

Rähni

et al. 2021

eLife

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Brain-derived neurotrophic factor (BDNF) controls the survival, growth, and function of neurons both during the development and in the adult nervous system. Bdnf is transcribed from several distinct promoters generating transcripts with alternative 5' exons. Bdnf transcripts initiated at the first cluster of exons have been associated with the regulation of body weight and various aspects of social behavior, but the mechanisms driving the expression of these transcripts have remained poorly understood. Here, we identify an evolutionarily conserved intronic enhancer region inside the Bdnf gene that regulates both basal and stimulus-dependent expression of the Bdnf transcripts starting from the first cluster of 5' exons in mouse and rat neurons. We further uncover a functional E-box element in the enhancer region, linking the expression of Bdnf and various pro-neural basic helix-loop-helix transcription factors. Collectively, our results shed new light on the cell-type- and stimulus-specific regulation of the important neurotrophic factor BDNF.

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Section: Methodsmentioning

confidence: 99%

Intronic enhancer region governs transcript-specific Bdnf expression in rodent neurons

Tuvikene

Esvald

Rähni

et al. 2021

eLife

Self Cite

View full text Add to dashboard Cite

show abstract

Intronic enhancer region governs transcript-specific BDNF expression in neurons

Tuvikene

Esvald

Rähni

et al. 2020

Preprint

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Brain-derived neurotrophic factor (BDNF) controls the survival, growth, and function of neurons both during the development and in the adult nervous system. BDNF gene is transcribed from several distinct promoters generating transcripts with alternative 5’ exons. BDNF transcripts initiated at the first cluster of exons have been associated with the regulation of body weight and various aspects of social behavior, but the mechanisms driving the expression of these transcripts have remained poorly understood. Here, we identify an evolutionarily conserved intronic enhancer region inside the BDNF gene that regulates both basal and stimulus-dependent expression of the BDNF transcripts starting from the first cluster of 5’ exons in neurons. We further uncover a functional E-box element in the enhancer region, linking the expression of BDNF and various pro-neural basic helix-loop-helix transcription factors. Collectively, our results shed new light on the cell type- and stimulus-specific regulation of the important neurotrophic factor BDNF.

show abstract

Usage of Bacterial Artificial Chromosomes for Studying BDNF Gene Regulation in Primary Cultures of Cortical Neurons and Astrocytes

Cited by 2 publications

References 38 publications

Intronic enhancer region governs transcript-specific Bdnf expression in rodent neurons

Intronic enhancer region governs transcript-specific Bdnf expression in rodent neurons

Intronic enhancer region governs transcript-specific BDNF expression in neurons

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