Usability of size-excluded fractions of soy protein hydrolysates for growth and viability of Chinese hamster ovary cells in protein-free suspension culture

Chun, Bok Hwan; Kim, Jong Hwang; Lee, Ho Joung; Chung, Namhyun

doi:10.1016/j.biortech.2006.04.012

Cited by 63 publications

(49 citation statements)

References 10 publications

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“…The presence of this peptone and particularly its interaction with the other hypeps seems to impact Vero cell growth. This result is in line with data reported by Chun et al 30 ; they demonstrated that for CHO cells, soy hydrolysate showed higher maximal cell density and growth rate in comparison with other hydrolysates.…”

Section: Discussionsupporting

confidence: 95%

Development of an animal‐component free medium for vero cells culture

Rourou

Ark²,

Velden³

et al. 2009

Biotechnology Progress

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This work describes the development of an animal-component free medium (IPT-AFM) that allows an optimal growth of Vero cells, an adherent cell line used for the production of viral vaccines. Statistical experimental design was applied to identify crucial nutrients that affect cell growth. Using Medium 199 or MEM as a basal medium, a serum-free medium (SFM) referred as IPT-SFM that only enclosed transferrin as a component of animal origin was developed at first. Then, the composition of IPT-SFM was further improved to obtain an animal-component free medium named IPT-AFM. IPT-AFM contains M199 as a basal medium, plant hydrolysates, epidermal growth factor, ethanolamine, ferric citrate, and vitamin C. Among various plant hydrolysates, specific combinations of soy (Hypep 1510) and wheat gluten (Hypeps 4601 and 4605) hydrolysates, were identified to promote cell growth; whereas individual Hypeps had a minor positive effect on cell growth. Nevertheless, the removal of serum did influence cell attachment. Coating tissue-culture flasks with teleostean, a product extracted from cold water fish skin, had not only enhanced cell attachment but also improved cell growth performance in static cultures. Different non-animal proteases were also assessed as an alternative to trypsin. TrypLE Select, a recombinant trypsin, gave the best cell growth performances. Kinetics of cell growth in IPT-AFM were investigated in T-flasks, cell growth was comparable with that obtained in MEM+10% fetal calf serum (FCS). A mean cell division number equal to 2.26 +/- 0.18 and a specific growth rate micro 0.019 +/- 0.003 h(-1) were achieved in IPT-AFM.

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Section: Discussionsupporting

confidence: 95%

Development of an animal‐component free medium for vero cells culture

Rourou

Ark²,

Velden³

et al. 2009

Biotechnology Progress

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“…Protein hydrolysates from animal products (primatone or peptone), microorganisms (yeast extract), and plants (peptones of soybean protein and wheat gluten) have been used to supplement basal medium as an additional source of nutrients such as amino acids, oligopeptides, lipids, and trace elements, in order to increase cell proliferation or productivity (Chun et al 2007;Franek et al 2000). Recently, plantderived protein hydrolysates have become commercially available and have proved to be very useful for producing human therapeutic proteins while eliminating the use of animal-derived proteins (Franek et al 2000;Schlaeger 1996).…”

Section: Introductionmentioning

confidence: 99%

Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium

Lee

Kim

et al. 2008

Biotechnol Lett

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Human keratinocytes are generally cultured in media containing bovine pituitary extract (BPE), an animal product that can be a source of infectious contaminants. We investigated whether a safer plant product could replace BPE in the culture medium. Medium containing both BPE and soy protein hydrolysates (Bacto Soytone and Soy Hydrolysate) produced the largest number of viable cells, followed in descending order by medium supplemented only with BPE, only with the hydrolysates, and without supplementation (basal medium only). Soybean protein is thus an excellent source of nutrients for the growth of adherent keratinocytes, although they do not fully substitute for BPE.

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“…Interestingly, while YE and plant protein peptones were widely used, no study reported the influence of YP on mammalian cell culture. However, not only the source of hydrolysates (Sung et al 2004;Kim and Lee 2009), but also the manufacturing process (Chun et al 2007) may affect cell growth and protein production. Furthermore, hydrolysates mixtures, concentrations and times of addition are also operating conditions that potentially impact cell culture (Sung et al 2004;Chun et al 2007;Kim and Lee 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Metabolic flux analyses showed that a peptone obtained from an animal source promoted the entry of pyruvate to TCA cycle (Bonarius et al 1996). Other studies underlined that peptones improved cell growth without increasing carbon source consumption and lactate production (Ballez et al 2004;Chun et al 2007), while ammonia production was slightly increased (Burteau et al 2003). Moreover, some authors underlined the potential use of lactate as a source of carbon after glucose depletion, triggered by plant hydrolysates (Burteau et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Combination of yeast hydrolysates to improve CHO cell growth and IgG production

et al. 2012

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Many studies underlined the great benefits of hydrolysates used as additives in animal free media on cell culture performances. However, to precisely define hydrolysate supplementation strategies, a deeper understanding of their effect on cell growth and protein production is required. In the present study, the effect of addition of one yeast extract (YE) and two yeast peptones (named YP.A and YP.B) in a chemically defined medium was first assessed on cell culture performances. Interestingly, specific effects were found depending on the degree of degradation of yeast hydrolysates. The YE at 1 g L -1 increased the maximal cell density by 70 %, while a mixture of YE (1 g L -1 ) and YP.A (4 g L -1 ) increased IgG production by 180 %. These conditions were then evaluated on the CHO cell kinetics all over cultures. Hydrolysates extended the cell growth phase in Erlenmeyer flask and increased the maximal growth rate in bioreactor up to 20 %. Cell growth stimulation induced by hydrolysates addition was linked with energetic metabolism improvement suggesting that they promote oxidative pathway. Furthermore, hydrolysates provided an additional source of substrate that supported cell growth despite glutamine limitation.

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Usability of size-excluded fractions of soy protein hydrolysates for growth and viability of Chinese hamster ovary cells in protein-free suspension culture

Cited by 63 publications

References 10 publications

Development of an animal‐component free medium for vero cells culture

Development of an animal‐component free medium for vero cells culture

Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium

Combination of yeast hydrolysates to improve CHO cell growth and IgG production

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