Usability of femoral head bone marrow to verify reference ranges for the assessment of myelodysplasia by flow cytometry

Gammeren, Adriaan J. van; Rijckevorsel, M van; Bras, A.E.; Brandt-Hagens, Claudia; Marvelde, Jeroen G. te; Visser, Carin de; Joosen, Annemiek M.C.P.; Ermens, A. A. M.; Velden, Vincent H.J. van der

doi:10.1111/ijlh.12911

Cited by 2 publications

(4 citation statements)

References 23 publications

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“…The Ogata scores with a cut‐off ≥2 have diagnostic and prognostic values (Della Porta et al, 2012 ; Della Porta et al, 2014 ). Scores of 2 are observed relatively often in patients with non‐clonal cytopenias, while scores 3 or 4 are significantly associated with cytomorphological myelodysplasia and MDS or MDS/MPN diagnosis (Rajab & Porwit, 2015 ; van Gammeren et al, 2018 ).…”

Section: Minimal Requirements To Assess Dysplasia By Mfcmentioning

confidence: 99%

“…Initially, a fourth DC subset, slanDC, was identified based on the expression of M-DC8 (6-sulfo LacNAc or slan) and CD16. Further studies showed that these SLAN+ cells are more closely related to monocytes than to DC and they were renamed as slan+ nc monocytes (van Leeuwen-Kerkhoff et al, 2017;2018). A decrease in the specific slan+ ncMo subset and increased CD1c + cDC (Meyerson et al, 2016) are features of CMML but their use in the diagnosis or prognostication of MDS is unclear (Van Leeuwen-Kerkhoff et al, 2021).…”

Section: Dendritic Cellsmentioning

confidence: 99%

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Multiparameter flow cytometry in the evaluation of myelodysplasia: Analytical issues

Porwit

Béné

Duetz

et al. 2022

Cytometry Part B Clinical

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Multiparameter flow cytometry (MFC) is one of the essential ancillary methods in bone marrow (BM) investigation of patients with cytopenia and suspected myelodysplastic syndrome (MDS). MFC can also be applied in the follow-up of MDS patients undergoing treatment. This document summarizes recommendations from the International/European Leukemia Net Working Group for Flow Cytometry in Myelodysplastic Syndromes (ELN iMDS Flow) on the analytical issues in MFC for the diagnostic work-up of MDS. Recommendations for the analysis of several BM cell subsets such as myeloid precursors, maturing granulocytic and monocytic components and erythropoiesis are given. A core set of 17 markers identified as independently related to a cytomorphologic diagnosis of myelodysplasia is suggested as mandatory for MFC evaluation of BM in a patient with cytopenia. A myeloid precursor cell (CD34 + CD19 À ) count >3% should be considered immunophenotypically indicative of myelodysplasia. However, MFC results should always be evaluated as part of an integrated hematopathology work-up. Looking forward, several machinelearning-based analytical tools of interest should be applied in parallel to

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Section: Minimal Requirements To Assess Dysplasia By Mfcmentioning

confidence: 99%

Section: Dendritic Cellsmentioning

confidence: 99%

Multiparameter flow cytometry in the evaluation of myelodysplasia: Analytical issues

Porwit

Béné

Duetz

et al. 2022

Cytometry Part B Clinical

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“…We demonstrate the femoral heads removed during THA provide abundant functional mononuclear and CD34 + cells as controls. A previous study reported on the utility of femoral head bone marrow for verification of cutoffs in flow cytometry evaluation of myelodysplastic syndromes [8]. Another study compared iliac crest, vertebral body, and femoral head MNCs as a source of mesenchymal stromal cells [9].…”

Section: Discussionmentioning

confidence: 99%

“…To identify alternative sources of hematopoietic cells as controls for studies of adult leukemia, we piloted the use of bone marrow cells extracted from the femoral heads of patients requiring elective total hip arthroplasty (THA). While we are not the first to use hematopoietic cells derived from femoral heads for laboratory studies [7][8][9], we are the first to report the abundances of cells derived from this unique source. We demonstrate that sufficient cells are recovered to perform functional studies, justifying the investment of time and resources required to establish the logistics.…”

Section: Introductionmentioning

confidence: 99%

Femoral Heads from Total Hip Arthroplasty as a Source of Adult Hematopoietic Cells

et al. 2021

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Normal human bone marrow cells are critical for studies of hematopoiesis and as controls to assess toxicity. As cells from commercial vendors are expensive, many laboratories resort to cancer-free bone marrow specimens obtained during staging or to umbilical cord blood cells, which may be abnormal or reflect a much younger age group compared to the disease samples under study. We piloted the use of femoral heads as an alternative and inexpensive source of normal bone marrow. Femoral heads were obtained from 21 successive patients undergoing elective hip arthroplasty. Mononuclear cells (MNCs) were purified with Ficoll, and CD3+, CD14+, and CD34+ cells were purified with antibody-coated microbeads. The median yield of MNCs was 8.95 × 107 (range, 1.62 × 105–2.52 × 108), and the median yield of CD34+ cells was 1.40 × 106 (range, 3.60 × 105–9.90 × 106). Results of downstream applications including qRT-PCR, colony-forming assays, and ex vivo proliferation analysis were of high quality and comparable to those obtained with standard bone marrow aspirates. We conclude that femoral heads currently discarded as medical waste are a cost-efficient source of bone marrow cells for research use.

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Usability of femoral head bone marrow to verify reference ranges for the assessment of myelodysplasia by flow cytometry

Cited by 2 publications

References 23 publications

Multiparameter flow cytometry in the evaluation of myelodysplasia: Analytical issues

Multiparameter flow cytometry in the evaluation of myelodysplasia: Analytical issues

Femoral Heads from Total Hip Arthroplasty as a Source of Adult Hematopoietic Cells

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