Urovirulence Determinants in<i>Escherichia coli</i>Strains Causing Prostatitis

Andreu, Antònia; Stapleton, Ann E.; Fennell, Cynthia L.; Lockman, Hank; Xercavins, Mariona; Fernández, Francisco Pedro García; Stamm, Walter E.

doi:10.1086/514065

Cited by 86 publications

(13 citation statements)

References 19 publications

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“…Finally, in the present study, the presence of toxins was significantly higher in isolates from men compared to women, and in isolates of ST73 compared to ST69. A high frequency of toxin producing in UTI isolates from men was also noticed by others (Andreu et al 1997). …”

Section: Discussionsupporting

confidence: 81%

Escherichia coli sequence type 73 as a cause of community acquired urinary tract infection in men and women in Rio de Janeiro, Brazil

da-Silva

Sousa

Martins

et al. 2017

Diagnostic Microbiology and Infectious Disease

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Escherichia coli clones ST131, ST69, ST95 and ST73 are frequent causes of urinary tract infections (UTI) and bloodstream infections. Specific clones and virulence profiles of E. coli causing UTI in men has been rarely described. The aim of this study was to characterize patient and clonal characteristics of community-acquired UTI caused by E. coli in men (n=12) and women (n=127) in Rio de Janeiro, Brazil, complementing a previous work. We characterized isolates in phylogenetic groups, ERIC2-PCR and PFGE types, MLST, genome similarity and virulence gene-profiles. UTI from men were more frequently caused by phylogenetic group B2 isolates (83% vs 42%, respectively, p 0.01), a group with significantly higher virulence scores compared with women. ST73 was the predominant clone in men (50%) and the second most frequent in women (12%), with the highest virulence score (mean and median=9) among other clones. ST73 gnomes formed at least six clusters. E. coli from men carried significantly higher numbers of virulence genes, such as sfa/focDE (67% vs 27%), hlyA (58% vs 24%), cnf 1 (58% vs 16%), fyuA (100% vs 82%) and MalX (92% vs 44%), compared with isolates from women. These data suggest the predominance and spread of ST73 isolates likely relates to an abundance of virulence determinants.

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Section: Discussionsupporting

confidence: 81%

Escherichia coli sequence type 73 as a cause of community acquired urinary tract infection in men and women in Rio de Janeiro, Brazil

da-Silva

Sousa

Martins

et al. 2017

Diagnostic Microbiology and Infectious Disease

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“…S3 in the supplemental material). We assayed four available, previously described strain collections, the ECOR (16), Andreu prostatitis (17), Johnson urosepsis (18), and Mand STEC (9) collections. On the basis of these analyses, we observed that some strains have iraL alone (like CFT073), some have iraM alone (like K-12), some have both iraL and iraM , and others have neither iraL nor iraM (Fig.…”

Section: Resultsmentioning

confidence: 99%

IraL Is an RssB Anti-adaptor That Stabilizes RpoS during Logarithmic Phase Growth in Escherichia coli and Shigella

Hryckowian

Battesti

Lemke

et al. 2014

mBio

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RpoS (σS), the general stress response sigma factor, directs the expression of genes under a variety of stressful conditions. Control of the cellular σS concentration is critical for appropriately scaled σS-dependent gene expression. One way to maintain appropriate levels of σS is to regulate its stability. Indeed, σS degradation is catalyzed by the ClpXP protease and the recognition of σS by ClpXP depends on the adaptor protein RssB. Three anti-adaptors (IraD, IraM, and IraP) exist in Escherichia coli K-12; each interacts with RssB and inhibits RssB activity under different stress conditions, thereby stabilizing σS. Unlike K-12, some E. coli isolates, including uropathogenic E. coli strain CFT073, show comparable cellular levels of σS during the logarithmic and stationary growth phases, suggesting that there are differences in the regulation of σS levels among E. coli strains. Here, we describe IraL, an RssB anti-adaptor that stabilizes σS during logarithmic phase growth in CFT073 and other E. coli and Shigella strains. By immunoblot analyses, we show that IraL affects the levels and stability of σS during logarithmic phase growth. By computational and PCR-based analyses, we reveal that iraL is found in many E. coli pathotypes but not in laboratory-adapted strains. Finally, by bacterial two-hybrid and copurification analyses, we demonstrate that IraL interacts with RssB by a mechanism distinct from that used by other characterized anti-adaptors. We introduce a fourth RssB anti-adaptor found in E. coli species and suggest that differences in the regulation of σS levels may contribute to host and niche specificity in pathogenic and nonpathogenic E. coli strains.

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“…In humans, the hemolysin phenotype, cnf1 gene, and P fimbriae have been found to be associated with prostatitis. 1 …”

Section: Discussionmentioning

confidence: 99%

Phylogroup and virulence gene association with clinical characteristics of Escherichia coli urinary tract infections from dogs and cats

et al. 2017

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Escherichia coli isolates from infections outside the gastrointestinal tract are termed extra-intestinal pathogenic E. coli (ExPEC) and can be divided into different subpathotypes; one of these is uropathogenic E. coli (UPEC). The frequency with which UPEC strains cause urinary tract infections in dogs and cats is not well documented. We used an oligonucleotide microarray to characterize 60 E. coli isolates associated with the urinary tract of dogs (n = 45) and cats (n = 15), collected from 2004 to 2007, into ExPEC and UPEC and to correlate results with patient clinical characteristics. Microarray analysis was performed, and phylogroup was determined by a quadruplex PCR assay. Isolates that were missing 1 or 2 of the gene determinants representative of a function (capsule, iron uptake related genes, or specific adhesins) were designated as “non-classifiable” by microarray. Phylogroup B2 was positively associated with the UPEC subpathotype (p < 0.0005) and negatively associated with “non-classifiable” isolates (p < 0.0005). Phylogroup D was positively associated with ExPEC pathotype (p = 0.025) and negatively associated with UPEC subpathotype (p = 0.014). The ExPEC pathotype was positively associated with hospitalization for one or more days (p = 0.031). The UPEC subpathotype was negatively associated with previous antimicrobial therapy (p = 0.045) and previous hospitalization within the 3 mo prior to the positive culture (p = 0.041). The UPEC subpathotype was positively associated with prostatitis (p = 0.073) and negatively associated with current immunosuppressive therapy (p = 0.090). Our results indicate that the case history observations may be critically important during the interpretation of laboratory results to encourage judicious use of antimicrobials.

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Urovirulence Determinants inEscherichia coliStrains Causing Prostatitis

Cited by 86 publications

References 19 publications

Escherichia coli sequence type 73 as a cause of community acquired urinary tract infection in men and women in Rio de Janeiro, Brazil

Escherichia coli sequence type 73 as a cause of community acquired urinary tract infection in men and women in Rio de Janeiro, Brazil

IraL Is an RssB Anti-adaptor That Stabilizes RpoS during Logarithmic Phase Growth in Escherichia coli and Shigella

Phylogroup and virulence gene association with clinical characteristics of Escherichia coli urinary tract infections from dogs and cats

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