Uroprotective effect of ambroxol in cyclophosphamide-induced cystitis in mice

Barut, Elif Nur; Engin, Seçkin; Barut, Burak; Kaya, Cansu; Keri̇moğlu, Gökçen; Özel, Arzu; Kadıoğlu, Mine

doi:10.1007/s11255-019-02128-y

Cited by 28 publications

(20 citation statements)

References 39 publications

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“…The present study revealed that induction of prostatitis led to reduced muscarinic receptor-induced bladder contractions. This finding is consistent with several previous studies on chemically induced, that is, nonbacterial, cystitis that display decreased muscarinic receptor-induced bladder contractility, 17,23,24 further highlighting that prostate-to-bladder cross-sensitization during CPPS leads to a situation in the bladder that is similar to what is seen in models of interstitial cystitis/bladder pain syndrome (IC/BPS). More surprisingly, the purinoceptor-induced response was also reduced.…”

Section: Histopathologysupporting

confidence: 91%

Prostate‐to‐bladder cross‐sensitization in a model of zymosan‐induced chronic pelvic pain syndrome in rats

et al. 2021

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Background: The aim of the present study was to investigate the effects of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) on bladder function and pathophysiology. Methods: To create a model for CPPS, rats were intraprostatically injected with zymosan or saline, serving as control. Metabolic cage experiments were performed 7, 14, or 21 days after zymosan injection and after 14 days in the control group. Thereafter, cystometry was performed in which simulated micturition cycles were induced by saline infusion and contractile responses to the cholinergic agonist methacholine and the purinergic agonist ATP were measured. Following cystometry, the prostate and urinary bladder were excised and assessed histopathologically for possible inflammatory changes. Results: Metabolic cage data revealed a significantly increased urinary frequency in zymosan treated rats. Likewise, the volume per micturition was significantly lower in all CPPS groups compared to controls. Cystometry showed a significant increase in the number of nonvoiding contractions, longer voiding time, and a trend towards lower compliance in CPPS rats compared to controls. Induction of CPPS led to significantly reduced cholinergic and purinergic contractile responses. Histopathological analysis demonstrated prostatic inflammation in all CPPS groups, in particular in later stage groups. Both the extent and grade of bladder inflammation were significantly higher in CPPS groups compared to controls. Conclusions: The current findings demonstrate a potential prostate-to-bladder cross-sensitization leading to symptoms of bladder overactivity and signs of bladder inflammation. Future clinical studies are required to verify the outcomes of the current study and enable advancement of patient care.

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Section: Histopathologysupporting

confidence: 91%

Prostate‐to‐bladder cross‐sensitization in a model of zymosan‐induced chronic pelvic pain syndrome in rats

et al. 2021

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“…In our study, we performed histologic staining with H & E. The findings here support our biochemical results. Very intense hemorrhagic foci were observed in the CP group, while appearances close to the control group were observed in the NaHS50 group especially [29]. We used APAF -1, Caspase -3 and Acrolein staining for immunohistochemical studies.…”

Section: Discussionmentioning

confidence: 99%

Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide

Özatik¹,

Özatik²,

Tekşen³

et al. 2021

Turk J Med Sci

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Backround and aim Cyclophosphamide (CP) is a drug used for treatment of many malignant diseases. However, it can cause serious side effects such as hemorrhagic cystitis and male infertility. Hydrogen sulfide (H2S) is a gaseous mediator and is suggested to have antioxidant, anti-inflammatory and antiapoptotic effects. In this study, dose-dependent effects of H2S donor sodium hydrosulfide (NaHS) on cyclophosphamide-induced hemorrhagic cystitis and testicular dysfunction were investigated in rats. Material and methods Rats were divided into 5 groups (n = 8): control, CP, NaHS25 μmol / kg, NaHS50 μmol / kg, NaHS100 μmol / kg. After treatment for 7 days intraperitoneally (ip), a single ip dose of CP 200 mg / kg was given on the 8th day. Then, treatment was continued for 7 days. In bladder and testicular tissues, IL 6, IL 10, cGMP, NO, H2S, FSH, LH and Testosterone levels were measured by ELISA. Histopathological examination with H & E staining as well as immunohistochemical staining for acrolein in bladder and Caspase-3 and APAF-1 in testis were performed. Results NaHS prevented the increased IL 6 and IL 10 values induced by CP as well as prevented the decrease in cGMP values associated with CP. There was no significant change in FSH values, but the LH value, which increased with CP, decreased with 25, 50 and 100 µmol 2 / kg NaHS. In contrast, testosterone decreased in the CP group and increased in the treatment groups. NaHS was effective in many biochemical and histopathological parameters at 25 and 50 µmol / kg doses, and this effect decreased at 100 µmol / kg dose. Conclusion H2S has a protective and therapeutic effect on hemorrhagic cystitis and testicular dysfunction induced by cyclophosphamide. It can be suggested that H2S is a promising molecule in facilitating cancer treatment.

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“…It was reported that CYP treatment exhibited a marked reduction in Ach-induced cholinergic contraction (Barut et al, 2019). A study of Al-Yahya and his colleagues reported that the responsiveness of the isolated urinary bladder rings from animals treated with CYP to Ach was markedly decreased when compared to the control group (Al-Yahya et al, 2009).…”

Section: Effects Of Cypmentioning

confidence: 99%

Uroprotective mechanisms of natural products against cyclophosphamide-induced urinary bladder toxicity: A comprehensive review

Sherif

2020

Acta Sci Pol Technol Aliment

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One of the widely used anticancer drugs for the treatment of various neoplasms is cyclophosphamide (CYP). The inactive prodrug CYP is metabolized by cytochrome P450 enzyme into active metabolites, phosphoramide mustard and acrolein. The accumulation of acrolein metabolite inside the urothelium results in hemorrhagic cystitis (HC) which is a urotoxic adverse effect associated with the use of CYP. To counteract the occurrence of HC induced by CYP, Mesna is usually used, with allergic reactions reported in some cases. Therefore, several natural products have drawn much attention as alternative safe therapies to reduce the urotoxicity produced from the use of CYP. This review will focus on certain uroprotective mechanisms related to some medicinal plants that are used to ameliorate the CYP-induced urotoxicity in experimental models. The mechanisms involving oxidative stress, inflammation, immune system, apoptosis, DNA fragmentation, uroplakins, purinergic signaling and muscarinic receptors, and CytoP450 metabolism are discussed.

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Uroprotective effect of ambroxol in cyclophosphamide-induced cystitis in mice

Cited by 28 publications

References 39 publications

Prostate‐to‐bladder cross‐sensitization in a model of zymosan‐induced chronic pelvic pain syndrome in rats

Prostate‐to‐bladder cross‐sensitization in a model of zymosan‐induced chronic pelvic pain syndrome in rats

Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide

Uroprotective mechanisms of natural products against cyclophosphamide-induced urinary bladder toxicity: A comprehensive review

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