Uronate Peaks and Urinary Hyaluronic Acid Levels Correlate With Interstitial Cystitis Severity

Lokeshwar, Vinata B.; Selzer, Marie G.; Unwala, Darius J.; Estrella, Verónica; Gómez, María Fernanda; Golshani, Roozbeh; Kester, Robert R.; Klumpp, David J.; Gousse, Angelo E.

doi:10.1016/j.juro.2006.04.012

Cited by 24 publications

(16 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Urinary GAG content in PBS/IC patients has been shown to be decreased (25,26), similar (27,28) and increased (4,13,18). Our results showed a significant decrease in urinary excretion of sulfated GAG of patients with PBS/IC compared to the controls.…”

Section: Discussionmentioning

confidence: 98%

“…Urine and tissue levels of GAG have been studied in PBS/IC and in animal models but their results are not uniform (2,(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). Such variation suggests that there is no complete understanding of how and why GAG is produced and degraded in PBS/IC, if GAG expression is the cause or the reaction for PBS/IC.…”

Section: Introductionmentioning

confidence: 99%

“…Or both cause and reaction. The mechanisms of changes in GAG levels that can occur during the course of the syndrome are not precisely described although some have correlated disease severity to symptoms (4). We investigated GAG behavior in an attempt to understand its metabolism and the chemical reactions related to the expression of those polisaccharides in tissue and urine.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of the metabolism of glycosaminoglycans in patients with interstitial cystis

et al. 2014

View full text Add to dashboard Cite

ARTICLE INFO ______________________________________________________________ ______________________Introduction: Painful bladder syndrome/interstitial cystitis (PBS/IC) pathogenesis is not fully known, but evidence shows that glycosaminoglycans (GAG) of bladder urothelium can participate in its genesis. The loss of these compounds facilitates the contact of urine compounds with deeper portions of bladder wall triggering an inflammatory process. We investigated GAG in urine and tissue of PBS/IC and pure stress urinary incontinence (SUI) patients to better understand its metabolism. Materials and Methods: Tissue and urine of 11 patients with PBS/IC according to NIDDK criteria were compared to 11 SUI patients. Tissue samples were analyzed by histological, immunohistochemistry and immunofluorescence methods. Statistical analysis were performed using t Student test and Anova, considering significant when p < 0.05. Results: PBS/IC patients had lower concentration of GAG in urine when compared to SUI (respectively 0.45 ± 0.11 x 0.62 ± 0.13 mg/mg creatinine, p < 0.05). However, there was no reduction of the content of GAG in the urothelium of both groups. Immunofluorescence showed that PBS/IC patients had a stronger staining of TGF-beta, decorin (a proteoglycan of chondroitin/dermatan sulfate), fibronectin and hyaluronic acid. Conclusion: the results suggest that GAG may be related to the ongoing process of inflammation and remodeling of the dysfunctional urothelium that is present in the PBS/IC.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of the metabolism of glycosaminoglycans in patients with interstitial cystis

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Among the most relevant of which are adherence inhibition of immune complexes to polymorphonuclear cells [8], marked inhibition of leokocyte migration and aggregation depending on viscosity [9], regulation of fibroblast and endothelial cell proliferation [10], enhancement of connective tissue healing [11], creation of a barrier over membranes, thus regulating movement of solutes [12], and scavenging of reactive oxygen species present in abundance in synovial fluid of patients with rheumatoid arthritis [13]. A recent study by Lokeshwar et al [14] showed that urinary HA levels were related to the severity of the IC and may be useful for monitoring the disease. A flaw in this pilot study is that the patient decided the interval and the number of treatments, not basing it on any objective criteria.…”

Section: Discussionmentioning

confidence: 99%

Sequential hydrodistension and intravesical instillation of hyaluronic acid under general anaesthesia for treatment of refractory interstitial cystitis: a pilot study

2007

View full text Add to dashboard Cite

Pilot study looking at the combination of general anaesthetic hydrodistension and intravesical hyaluronic acid for treatment of refractory interstitial cystitis. Twenty-three treatment refractory patients were recruited with an average age 53.4 years. All underwent general anaesthetic cystoscopy, hydrodistension and instillation of hyaluronic acid (40 mg/50 ml). The bladder was then subsequently drained with the patient awake. Two initial treatments were carried out a month apart and duration between treatments increased depending upon symptom response. In the responders, the average number of treatments was 6.6 (median 4.5), duration between treatments was 3.1 months (median 2.6) and follow-up 15.8 months (median 16). Seventeen patients (74%) responded with immediate improvement in symptoms. In all responders, healing of ulceration and resolution of inflammation occurred. Average anaesthetic bladder capacity increased in the responder group from an average of 492 ml (median 500 ml) to an average of 776 ml (median 700 ml). Our pilot data suggests sequential hydrodistension and hyaluronic acid treatment under general anaesthesia may be considered for resistant cases of interstitial cystitis, especially those that cannot tolerate the instillation procedure under local anaesthesia. Further prospective trials are required.

show abstract

“…In general, sensitivities were much lower when only patients under surveillance were taken into account than when the patient cohorts included patients with primary disease or patients with highgrade tumours. The sensitivities (percentages in parentheses) of the markers for patients under surveillance in increasing order were: cytology (35), telomerase (39), Hb dipstick (40), BTA (48), FDP (54), Quanticyt (58), BTA stat (58), cytometry (60), UBC (60), TPS (65), Immunocyt (67), BTA trak (71), NMP22 (71), Lewis X (75), FISH (UroVysion, 79), microsatellite analysis (MA, 82), CK20 (85) and CYFRA 21-1 (85).…”

Section: Introductionmentioning

confidence: 99%

Detection of tumours of the urinary tract in voided urine

Zwarthoff

2008

Scandinavian Journal of Urology and Nephrology

View full text Add to dashboard Cite

Patients with non-muscle-invasive bladder cancer are treated by transurethral resection. About 60-70% of these patients will develop recurrences and in 11% of these cases progression to a muscle-invasive tumour occurs. Surveillance of patients by cystoscopy is therefore carried out every 3-4 months in the first 2 years and yearly thereafter. Several biomarkers have been developed that potentially can detect recurrent bladder cancer in voided urine samples and may present an alternative for the invasive cystoscopy procedure. Recently, van Rhijn reviewed the performance of several of these biomarkers regarding detection of recurrent disease in patients under surveillance. In general, sensitivities were much lower when only patients under surveillance were taken into account than when the patient cohorts included patients with primary disease or patients with high-grade tumours. In this article recent new data on those markers that displayed a sensitivity and specificity of at least 70% as mentioned in the review by van Rhijn are reviewed. The literature selected was limited to those papers in which the performance of makers was assayed only on urine samples of patients under surveillance. The markers with sensitivity and specificity over 70% that were selected from the previous study are Lewis X, NMP22, microsatellite analysis (MA), CYFRA 21.1, cytokeratin 20 and the UroVysion fluorescence in situ hybridization (FISH) test. Recent new developments such as the use of FGFR3 mutation analysis and methylation detection are also discussed. In conclusion, tests such as the UroVysion FISH test and MA are able to detect most concomitant recurrences and to predict recurrent disease. In general, lesions that are missed are pTa and low grade. With MA several upper tract recurrences were identified that were missed by cystoscopy. The value of the most promising urine tests needs to be established in longitudinal studies and exclusively on patients under surveillance for recurrent disease. A longitudinal setting allows subsequent urine samples to be tested and this increases sensitivity because a negative test outcome sometimes occurs between positive ones. Stratification of patients according to the genetic status of their primary tumours and smoking habits should be investigated. Decision models should be developed that recommend at which points in time cystoscopy or urine testing should be performed.

show abstract

Uronate Peaks and Urinary Hyaluronic Acid Levels Correlate With Interstitial Cystitis Severity

Abstract: Urinary glycosaminoglycan profile, uronate content and hyaluronic acid levels are potentially useful markers for monitoring interstitial cystitis severity, and are likely to be involved in interstitial cystitis pathophysiology.

Cited by 24 publications

References 19 publications

Evaluation of the metabolism of glycosaminoglycans in patients with interstitial cystis

Evaluation of the metabolism of glycosaminoglycans in patients with interstitial cystis

Sequential hydrodistension and intravesical instillation of hyaluronic acid under general anaesthesia for treatment of refractory interstitial cystitis: a pilot study

Detection of tumours of the urinary tract in voided urine

Contact Info

Product

Resources

About